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[Effect of electroacupuncture on spleen lymphocyte apoptosis-related gene expression in aging rats with yang deficiency].
Zhen Ci Yan Jiu. 2012 Aug; 37(4):266-70.ZC

Abstract

OBJECTIVE

To observe effects of electroacupuncture (EA) of "Guanyuan" (GV 4)-"Housanli" (ST 36)-"Baihui" (GV 20,"Shuanggu Yitong" acupoint prescription) on the expression of splenic lymphocyte apoptosis-related genes Fas, Bax, and Bcl-2 in aging rats with yang defiency.

METHODS

Thirty Sprague-Dawley aged female rats were randomly and e-ic qually divided into normal control, yang deficiency model (model) and EA groups. The yang deficiency model was established by subcutaneous injection of D-galactose (125 mg/kg) and hydrocortisone(1.5 mg/100 g). EA (2 Hz, 1 mA) was applied to "Guanyuan"(GV 4), "Housanli" (ST 36) and "Baihui" (GV 20) for 15 min, once daily, 6 times per week for 4 weeks. The expression of pro-apoptotic genes Fas, Bax and anti-apoptosis gene Bcl-2 in the spleen tissues were detected by real-time quantitative RT-PCR.

RESULTS

In comparison with the normal control group, splenic Fas mRNA and Bax mRNA expression levels were upregulated significantly, and Bcl-2 mRNA expression level was down-regulated considerably in the model group (P < 0. 01). After EA intervention, splenic Fas mRNA and Bax mRNA expression levels were down-regulated markedly, while Bcl-2 mRNA expression up-regulated remarkably in the EA group (P < 0.05, P < 0.01).

CONCLUSION

EA of acupoint prescription ["Guanyuan" (GV 4), "Housanli" (ST 36) and "Baihui" (GV 20)] can down-regulate spleen Fas mRNA and Bax mRNA expression, and up-regulate Bcl-2 mRNA expression in aging rats with yang deficiency, which may contribute to its effect in improving yang deficiency in clinic.

Authors+Show Affiliations

Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061, China. Hwang.TCM@china.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23140045

Citation

Wang, Hua, et al. "[Effect of Electroacupuncture On Spleen Lymphocyte Apoptosis-related Gene Expression in Aging Rats With Yang Deficiency]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 4, 2012, pp. 266-70.
Wang H, Mao HF, Liu JM, et al. [Effect of electroacupuncture on spleen lymphocyte apoptosis-related gene expression in aging rats with yang deficiency]. Zhen Ci Yan Jiu. 2012;37(4):266-70.
Wang, H., Mao, H. F., Liu, J. M., Hao, Q., Tang, H. T., & Li, J. (2012). [Effect of electroacupuncture on spleen lymphocyte apoptosis-related gene expression in aging rats with yang deficiency]. Zhen Ci Yan Jiu = Acupuncture Research, 37(4), 266-70.
Wang H, et al. [Effect of Electroacupuncture On Spleen Lymphocyte Apoptosis-related Gene Expression in Aging Rats With Yang Deficiency]. Zhen Ci Yan Jiu. 2012;37(4):266-70. PubMed PMID: 23140045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on spleen lymphocyte apoptosis-related gene expression in aging rats with yang deficiency]. AU - Wang,Hua, AU - Mao,Hui-Fang, AU - Liu,Jian-Min, AU - Hao,Qing, AU - Tang,Hong-Tu, AU - Li,Jia, PY - 2012/11/13/entrez PY - 2012/11/13/pubmed PY - 2013/1/9/medline SP - 266 EP - 70 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 4 N2 - OBJECTIVE: To observe effects of electroacupuncture (EA) of "Guanyuan" (GV 4)-"Housanli" (ST 36)-"Baihui" (GV 20,"Shuanggu Yitong" acupoint prescription) on the expression of splenic lymphocyte apoptosis-related genes Fas, Bax, and Bcl-2 in aging rats with yang defiency. METHODS: Thirty Sprague-Dawley aged female rats were randomly and e-ic qually divided into normal control, yang deficiency model (model) and EA groups. The yang deficiency model was established by subcutaneous injection of D-galactose (125 mg/kg) and hydrocortisone(1.5 mg/100 g). EA (2 Hz, 1 mA) was applied to "Guanyuan"(GV 4), "Housanli" (ST 36) and "Baihui" (GV 20) for 15 min, once daily, 6 times per week for 4 weeks. The expression of pro-apoptotic genes Fas, Bax and anti-apoptosis gene Bcl-2 in the spleen tissues were detected by real-time quantitative RT-PCR. RESULTS: In comparison with the normal control group, splenic Fas mRNA and Bax mRNA expression levels were upregulated significantly, and Bcl-2 mRNA expression level was down-regulated considerably in the model group (P < 0. 01). After EA intervention, splenic Fas mRNA and Bax mRNA expression levels were down-regulated markedly, while Bcl-2 mRNA expression up-regulated remarkably in the EA group (P < 0.05, P < 0.01). CONCLUSION: EA of acupoint prescription ["Guanyuan" (GV 4), "Housanli" (ST 36) and "Baihui" (GV 20)] can down-regulate spleen Fas mRNA and Bax mRNA expression, and up-regulate Bcl-2 mRNA expression in aging rats with yang deficiency, which may contribute to its effect in improving yang deficiency in clinic. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/23140045/[Effect_of_electroacupuncture_on_spleen_lymphocyte_apoptosis_related_gene_expression_in_aging_rats_with_yang_deficiency]_ L2 - https://medlineplus.gov/olderadulthealth.html DB - PRIME DP - Unbound Medicine ER -