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[Effects of electroacupuncture and manual acupuncture interventions on contents of serum inflammatory cytokines in rats with chronic adjuvant arthritis].
Zhen Ci Yan Jiu 2012; 37(4):271-6ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) or manual acupuncture of unilateral or bilateral "Zusanli" (ST 36) on the contents of serum inflammatory factors interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), and anti-inflammatory factors interleukin 2 (IL-2), interleukin 4 (IL-4) and interleukin 10 (IL-10), so as to reveal their mechanism underlying improvement of chronic arthritis.

METHODS

Sixty SD rats were randomly divided into normal control, model, electroacupuncture (EA)-left (L) Zusanli (ST 36), EA-bilateral (Bi)-ST 36,and manual acupuncture (MA)-L-ST 36 groups (12 rats/group). Arthritis model was established by subcutaneous injection of complete Freund's adjuvant (0.1 mL)into the hindpaw. EA (2 Hz/100 Hz, 1-3 mA, pulse duration: 0.6 ms/0.2 ms) or manual needle stimulation was applied to the left or bilateral "Zusanli" (ST 36) for 30 min, twice every week for 4 weeks. Enzyme linked immunosorbent assay (ELISA) was used to detect the contents of serum IL-1beta, TNF-alpha, IL-2, IL-4 and IL-10, respectively.

RESULTS

Compared with the normal control group, the contents of serum inflammatory factors IL-1beta and TNF-alpha, anti-inflammatory factors IL-4 and IL-10 were increased significantly in the model group (P < 0.001). In comparison with the model group, serum IL-1beta, TNF-alpha, IL-4 and IL-10 contents were down-regulated considerably in the EA-L-ST 36, EA-Bi-ST 36 and MA-L-ST 36 groups (P < 0.001, P < 0.005). Serum IL-2 content had no apparent changes after modeling in comparison with the normal group (P > 0.05). While serum IL-2 contents were markedly higher in the EA-L-ST 36, EA-Bi-ST 36 and MA-L-ST 36 groups than in the model group (P < 0.05). No significant differences were found among the EA-L-ST 36, EA-Bi-ST 36 and MA-L-ST 36 groups in serum IL-1beta, TNF-alpha, IL-2, IL-4 and IL-10 contents (P > 0.05).

CONCLUSION

EA or manual acupuncture stimulation of single or bilateral ST 36 can effectively down-regulate serum inflammatory factors IL-1beta and TNF-alpha levels, and upregulate anti-inflammatory factor IL-2 level in chronic arthritis rats, which may contribute to their effects in relieving chronic arthritis.

Authors+Show Affiliations

School of Acu-moxibustion and Massage, Beijing University of Chinese Medicine, Beijing 100029, China. agoodfriend@126.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23140046

Citation

Li, Wen-Xun, et al. "[Effects of Electroacupuncture and Manual Acupuncture Interventions On Contents of Serum Inflammatory Cytokines in Rats With Chronic Adjuvant Arthritis]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 4, 2012, pp. 271-6.
Li WX, Huang YR, Lei LD, et al. [Effects of electroacupuncture and manual acupuncture interventions on contents of serum inflammatory cytokines in rats with chronic adjuvant arthritis]. Zhen Ci Yan Jiu. 2012;37(4):271-6.
Li, W. X., Huang, Y. R., Lei, L. D., & Li, N. (2012). [Effects of electroacupuncture and manual acupuncture interventions on contents of serum inflammatory cytokines in rats with chronic adjuvant arthritis]. Zhen Ci Yan Jiu = Acupuncture Research, 37(4), pp. 271-6.
Li WX, et al. [Effects of Electroacupuncture and Manual Acupuncture Interventions On Contents of Serum Inflammatory Cytokines in Rats With Chronic Adjuvant Arthritis]. Zhen Ci Yan Jiu. 2012;37(4):271-6. PubMed PMID: 23140046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture and manual acupuncture interventions on contents of serum inflammatory cytokines in rats with chronic adjuvant arthritis]. AU - Li,Wen-Xun, AU - Huang,Yi-Ran, AU - Lei,Lin-Dan, AU - Li,Na, PY - 2012/11/13/entrez PY - 2012/11/13/pubmed PY - 2013/1/9/medline SP - 271 EP - 6 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 4 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) or manual acupuncture of unilateral or bilateral "Zusanli" (ST 36) on the contents of serum inflammatory factors interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), and anti-inflammatory factors interleukin 2 (IL-2), interleukin 4 (IL-4) and interleukin 10 (IL-10), so as to reveal their mechanism underlying improvement of chronic arthritis. METHODS: Sixty SD rats were randomly divided into normal control, model, electroacupuncture (EA)-left (L) Zusanli (ST 36), EA-bilateral (Bi)-ST 36,and manual acupuncture (MA)-L-ST 36 groups (12 rats/group). Arthritis model was established by subcutaneous injection of complete Freund's adjuvant (0.1 mL)into the hindpaw. EA (2 Hz/100 Hz, 1-3 mA, pulse duration: 0.6 ms/0.2 ms) or manual needle stimulation was applied to the left or bilateral "Zusanli" (ST 36) for 30 min, twice every week for 4 weeks. Enzyme linked immunosorbent assay (ELISA) was used to detect the contents of serum IL-1beta, TNF-alpha, IL-2, IL-4 and IL-10, respectively. RESULTS: Compared with the normal control group, the contents of serum inflammatory factors IL-1beta and TNF-alpha, anti-inflammatory factors IL-4 and IL-10 were increased significantly in the model group (P < 0.001). In comparison with the model group, serum IL-1beta, TNF-alpha, IL-4 and IL-10 contents were down-regulated considerably in the EA-L-ST 36, EA-Bi-ST 36 and MA-L-ST 36 groups (P < 0.001, P < 0.005). Serum IL-2 content had no apparent changes after modeling in comparison with the normal group (P > 0.05). While serum IL-2 contents were markedly higher in the EA-L-ST 36, EA-Bi-ST 36 and MA-L-ST 36 groups than in the model group (P < 0.05). No significant differences were found among the EA-L-ST 36, EA-Bi-ST 36 and MA-L-ST 36 groups in serum IL-1beta, TNF-alpha, IL-2, IL-4 and IL-10 contents (P > 0.05). CONCLUSION: EA or manual acupuncture stimulation of single or bilateral ST 36 can effectively down-regulate serum inflammatory factors IL-1beta and TNF-alpha levels, and upregulate anti-inflammatory factor IL-2 level in chronic arthritis rats, which may contribute to their effects in relieving chronic arthritis. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/23140046/[Effects_of_electroacupuncture_and_manual_acupuncture_interventions_on_contents_of_serum_inflammatory_cytokines_in_rats_with_chronic_adjuvant_arthritis]_ L2 - http://www.diseaseinfosearch.org/result/592 DB - PRIME DP - Unbound Medicine ER -