[Effect of electroacupuncture on expression of proliferating cell nuclear antigen and glial fibrillary acidic protein in subventricular zone of Parkinson's disease rats].
Abstract
OBJECTIVE
To observe the effect of electroacupuncture (EA) on the expression of proliferating cell nuclear antigen (PCNA) and glial fibrillary acidic protein (GFAP) in the subventricular zone (SVZ) of Parkinson's disease (PD) rats, so as to explore its mechanisms underlying improvement of PD.METHODS
Eighteen SD rats were randomized into normal control, model and EA groups (6 rats/group). PD model was duplicated by microinjection of 6-hydroxydopamine (2.5 microg/microL, 6 microL) containing 0.02% antiscorbic acid into the right medial forebrain bundle region (P: -4. 0, L: 0.8, H: 8.0). On the 7th day after modeling, EA (100 Hz) was applied to bilateral "Hegu" (LI 4) and "Taichong" (LR 3) for 30 min, once a day for 21 days. The expression levels of tyrosine hydroxylase (TH), GFAP and PCNA in the right SVZ tissue were detected by immunohistochemistry.RESULTS
TH expression was strong in the normal control group, but no TH expression was found in the model and EA groups. In comparison with the normal control group, the expression levels of PCNA and GFAP proteins and the number of PCNA-IR-positive cells in the right SVZ were decreased significantly in the model group (P < 0.01); while compared with the model group, the expression levels of PCNA and GFAP proteins and the number of PCNA-IR-positive cells in the right SVZ were up-regulated considerably in the EA group (P < 0.01, P < 0.05).CONCLUSION
EA can up-regulate the expression of PCNA and GFAP proteins in the SVZ in PD rats without changes of TH expression, which may contribute to its effect in improving clinical symptoms of PD.Authors+Show Affiliations
,Wannan Medical College, Wuhu 241002, China.
,Source
Zhen ci yan jiu = Acupuncture research 37:4 2012 Aug pg 286-90
MeSH
AnimalsBrain
Electroacupuncture
Glial Fibrillary Acidic Protein
Humans
Male
Parkinson Disease
Proliferating Cell Nuclear Antigen
Rats
Rats, Sprague-Dawley
Pub Type(s)
English AbstractJournal Article
Research Support, Non-U.S. Gov't
Language
chi
PubMed ID
23140049
Citation
Ding, Yan-Xia, et al. "[Effect of Electroacupuncture On Expression of Proliferating Cell Nuclear Antigen and Glial Fibrillary Acidic Protein in Subventricular Zone of Parkinson's Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 4, 2012, pp. 286-90.
Ding YX, Hou LQ, Xiong KR. [Effect of electroacupuncture on expression of proliferating cell nuclear antigen and glial fibrillary acidic protein in subventricular zone of Parkinson's disease rats]. Zhen Ci Yan Jiu. 2012;37(4):286-90.
Ding, Y. X., Hou, L. Q., & Xiong, K. R. (2012). [Effect of electroacupuncture on expression of proliferating cell nuclear antigen and glial fibrillary acidic protein in subventricular zone of Parkinson's disease rats]. Zhen Ci Yan Jiu = Acupuncture Research, 37(4), pp. 286-90.
Ding YX, Hou LQ, Xiong KR. [Effect of Electroacupuncture On Expression of Proliferating Cell Nuclear Antigen and Glial Fibrillary Acidic Protein in Subventricular Zone of Parkinson's Disease Rats]. Zhen Ci Yan Jiu. 2012;37(4):286-90. PubMed PMID: 23140049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - [Effect of electroacupuncture on expression of proliferating cell nuclear antigen and glial fibrillary acidic protein in subventricular zone of Parkinson's disease rats].
AU - Ding,Yan-Xia,
AU - Hou,Liang-Qin,
AU - Xiong,Ke-Ren,
PY - 2012/11/13/entrez
PY - 2012/11/13/pubmed
PY - 2013/1/9/medline
SP - 286
EP - 90
JF - Zhen ci yan jiu = Acupuncture research
JO - Zhen Ci Yan Jiu
VL - 37
IS - 4
N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on the expression of proliferating cell nuclear antigen (PCNA) and glial fibrillary acidic protein (GFAP) in the subventricular zone (SVZ) of Parkinson's disease (PD) rats, so as to explore its mechanisms underlying improvement of PD. METHODS: Eighteen SD rats were randomized into normal control, model and EA groups (6 rats/group). PD model was duplicated by microinjection of 6-hydroxydopamine (2.5 microg/microL, 6 microL) containing 0.02% antiscorbic acid into the right medial forebrain bundle region (P: -4. 0, L: 0.8, H: 8.0). On the 7th day after modeling, EA (100 Hz) was applied to bilateral "Hegu" (LI 4) and "Taichong" (LR 3) for 30 min, once a day for 21 days. The expression levels of tyrosine hydroxylase (TH), GFAP and PCNA in the right SVZ tissue were detected by immunohistochemistry. RESULTS: TH expression was strong in the normal control group, but no TH expression was found in the model and EA groups. In comparison with the normal control group, the expression levels of PCNA and GFAP proteins and the number of PCNA-IR-positive cells in the right SVZ were decreased significantly in the model group (P < 0.01); while compared with the model group, the expression levels of PCNA and GFAP proteins and the number of PCNA-IR-positive cells in the right SVZ were up-regulated considerably in the EA group (P < 0.01, P < 0.05). CONCLUSION: EA can up-regulate the expression of PCNA and GFAP proteins in the SVZ in PD rats without changes of TH expression, which may contribute to its effect in improving clinical symptoms of PD.
SN - 1000-0607
UR - https://www.unboundmedicine.com/medline/citation/23140049/
L2 - https://medlineplus.gov/parkinsonsdisease.html
DB - PRIME
DP - Unbound Medicine
ER -
