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Genotoxicity of polyvinylpyrrolidone-coated silver nanoparticles in BEAS 2B cells.
Toxicology. 2013 Nov 08; 313(1):38-48.T

Abstract

Silver nanoparticles (AgNPs) are widely utilized in various consumer products and medical devices, especially due to their antimicrobial properties. However, several studies have associated these particles with toxic effects, such as inflammation and oxidative stress in vivo and cytotoxic and genotoxic effects in vitro. Here, we assessed the genotoxic effects of AgNPs coated with polyvinylpyrrolidone (PVP) (average diameter 42.5±14.5 nm) on human bronchial epithelial BEAS 2B cells in vitro. AgNPs were dispersed in bronchial epithelial growth medium (BEGM) with 0.6 mg/ml bovine serum albumin (BSA). The AgNP were partially well-dispersed in the medium and only limited amounts (ca. 0.02 μg Ag(+) ion/l) could be dissolved after 24h. The zeta-potential of the AgNPs was found to be highly negative in pure water but was at least partially neutralized in BEGM with 0.6 mg BSA/ml. Cytotoxicity was measured by cell number count utilizing Trypan Blue exclusion and by an ATP-based luminescence cell viability assay. Genotoxicity was assessed by the alkaline single cell gel electrophoresis (comet) assay, the cytokinesis-block micronucleus (MN) assay, and the chromosomal aberration (CA) assay. The cells were exposed to various doses (0.5-48 μg/cm(2) corresponding to 2.5-240 μg/ml) of AgNPs for 4 and 24 h in the comet assay, for 48 h in the MN assay, and for 24 and 48 h in the CA assay. DNA damage measured by the percent of DNA in comet tail was induced in a dose-dependent manner after both the 4-h and the 24-h exposures to AgNPs, with a statistically significant increase starting at 16 μg/cm(2) (corresponding to 60.8 μg/ml) and doubling of the percentage of DNA in tail at 48 μg/cm(2). However, no induction of MN or CAs was observed at any of the doses or time points. The lack of induction of chromosome damage by the PVP-coated AgNPs is possibly due to the coating which may protect the cells from direct interaction with the AgNPs, either by reducing ion leaching from the particles or by causing extensive agglomeration of the nanoparticles, with a possible reduction of the cellular uptake.

Authors+Show Affiliations

Nanosafety Research Center and Safe New Technologies, Work Environment Development, Finnish Institute of Occupational Health, Helsinki, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23142790

Citation

Nymark, Penny, et al. "Genotoxicity of Polyvinylpyrrolidone-coated Silver Nanoparticles in BEAS 2B Cells." Toxicology, vol. 313, no. 1, 2013, pp. 38-48.
Nymark P, Catalán J, Suhonen S, et al. Genotoxicity of polyvinylpyrrolidone-coated silver nanoparticles in BEAS 2B cells. Toxicology. 2013;313(1):38-48.
Nymark, P., Catalán, J., Suhonen, S., Järventaus, H., Birkedal, R., Clausen, P. A., Jensen, K. A., Vippola, M., Savolainen, K., & Norppa, H. (2013). Genotoxicity of polyvinylpyrrolidone-coated silver nanoparticles in BEAS 2B cells. Toxicology, 313(1), 38-48. https://doi.org/10.1016/j.tox.2012.09.014
Nymark P, et al. Genotoxicity of Polyvinylpyrrolidone-coated Silver Nanoparticles in BEAS 2B Cells. Toxicology. 2013 Nov 8;313(1):38-48. PubMed PMID: 23142790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity of polyvinylpyrrolidone-coated silver nanoparticles in BEAS 2B cells. AU - Nymark,Penny, AU - Catalán,Julia, AU - Suhonen,Satu, AU - Järventaus,Hilkka, AU - Birkedal,Renie, AU - Clausen,Per Axel, AU - Jensen,Keld Alstrup, AU - Vippola,Minnamari, AU - Savolainen,Kai, AU - Norppa,Hannu, Y1 - 2012/11/08/ PY - 2012/06/06/received PY - 2012/09/28/accepted PY - 2012/11/13/entrez PY - 2012/11/13/pubmed PY - 2013/12/16/medline KW - Chromosomal damage KW - DNA damage KW - Human bronchial epithelial cells KW - Micronuclei KW - Silver nanoparticles SP - 38 EP - 48 JF - Toxicology JO - Toxicology VL - 313 IS - 1 N2 - Silver nanoparticles (AgNPs) are widely utilized in various consumer products and medical devices, especially due to their antimicrobial properties. However, several studies have associated these particles with toxic effects, such as inflammation and oxidative stress in vivo and cytotoxic and genotoxic effects in vitro. Here, we assessed the genotoxic effects of AgNPs coated with polyvinylpyrrolidone (PVP) (average diameter 42.5±14.5 nm) on human bronchial epithelial BEAS 2B cells in vitro. AgNPs were dispersed in bronchial epithelial growth medium (BEGM) with 0.6 mg/ml bovine serum albumin (BSA). The AgNP were partially well-dispersed in the medium and only limited amounts (ca. 0.02 μg Ag(+) ion/l) could be dissolved after 24h. The zeta-potential of the AgNPs was found to be highly negative in pure water but was at least partially neutralized in BEGM with 0.6 mg BSA/ml. Cytotoxicity was measured by cell number count utilizing Trypan Blue exclusion and by an ATP-based luminescence cell viability assay. Genotoxicity was assessed by the alkaline single cell gel electrophoresis (comet) assay, the cytokinesis-block micronucleus (MN) assay, and the chromosomal aberration (CA) assay. The cells were exposed to various doses (0.5-48 μg/cm(2) corresponding to 2.5-240 μg/ml) of AgNPs for 4 and 24 h in the comet assay, for 48 h in the MN assay, and for 24 and 48 h in the CA assay. DNA damage measured by the percent of DNA in comet tail was induced in a dose-dependent manner after both the 4-h and the 24-h exposures to AgNPs, with a statistically significant increase starting at 16 μg/cm(2) (corresponding to 60.8 μg/ml) and doubling of the percentage of DNA in tail at 48 μg/cm(2). However, no induction of MN or CAs was observed at any of the doses or time points. The lack of induction of chromosome damage by the PVP-coated AgNPs is possibly due to the coating which may protect the cells from direct interaction with the AgNPs, either by reducing ion leaching from the particles or by causing extensive agglomeration of the nanoparticles, with a possible reduction of the cellular uptake. SN - 1879-3185 UR - https://www.unboundmedicine.com/medline/citation/23142790/Genotoxicity_of_polyvinylpyrrolidone_coated_silver_nanoparticles_in_BEAS_2B_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(12)00369-1 DB - PRIME DP - Unbound Medicine ER -