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Cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile in Arabidopsis.
Plant Cell 2012; 24(11):4621-34PC

Abstract

In Arabidopsis thaliana, DES1 is the only identified L-Cysteine desulfhydrase located in the cytosol, and it is involved in the degradation of cysteine and the concomitant production of H(2)S in this cell compartment. Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided insight into the role of sulfide metabolically generated in the cytosol as a signaling molecule. Mutations of L-CYS DESULFHYDRASE 1 (DES1) impede H(2)S generation in the Arabidopsis cytosol and strongly affect plant metabolism. Senescence-associated vacuoles are detected in mesophyll protoplasts of des1 mutants. Additionally, DES1 deficiency promotes the accumulation and lipidation of the ATG8 protein, which is associated with the process of autophagy. The transcriptional profile of the des1-1 mutant corresponds to its premature senescence and autophagy-induction phenotypes, and restoring H(2)S generation has been shown to eliminate the phenotypic defects of des1 mutants. Moreover, sulfide is able to reverse ATG8 accumulation and lipidation, even in wild-type plants when autophagy is induced by carbon starvation, suggesting a general effect of sulfide on autophagy regulation that is unrelated to sulfur or nitrogen limitation stress. Our results suggest that cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile of Arabidopsis.

Authors+Show Affiliations

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092 Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23144183

Citation

Álvarez, Consolación, et al. "Cysteine-generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in Arabidopsis." The Plant Cell, vol. 24, no. 11, 2012, pp. 4621-34.
Álvarez C, García I, Moreno I, et al. Cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile in Arabidopsis. Plant Cell. 2012;24(11):4621-34.
Álvarez, C., García, I., Moreno, I., Pérez-Pérez, M. E., Crespo, J. L., Romero, L. C., & Gotor, C. (2012). Cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile in Arabidopsis. The Plant Cell, 24(11), pp. 4621-34. doi:10.1105/tpc.112.105403.
Álvarez C, et al. Cysteine-generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in Arabidopsis. Plant Cell. 2012;24(11):4621-34. PubMed PMID: 23144183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile in Arabidopsis. AU - Álvarez,Consolación, AU - García,Irene, AU - Moreno,Inmaculada, AU - Pérez-Pérez,María Esther, AU - Crespo,José L, AU - Romero,Luis C, AU - Gotor,Cecilia, Y1 - 2012/11/09/ PY - 2012/11/13/entrez PY - 2012/11/13/pubmed PY - 2013/8/22/medline SP - 4621 EP - 34 JF - The Plant cell JO - Plant Cell VL - 24 IS - 11 N2 - In Arabidopsis thaliana, DES1 is the only identified L-Cysteine desulfhydrase located in the cytosol, and it is involved in the degradation of cysteine and the concomitant production of H(2)S in this cell compartment. Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided insight into the role of sulfide metabolically generated in the cytosol as a signaling molecule. Mutations of L-CYS DESULFHYDRASE 1 (DES1) impede H(2)S generation in the Arabidopsis cytosol and strongly affect plant metabolism. Senescence-associated vacuoles are detected in mesophyll protoplasts of des1 mutants. Additionally, DES1 deficiency promotes the accumulation and lipidation of the ATG8 protein, which is associated with the process of autophagy. The transcriptional profile of the des1-1 mutant corresponds to its premature senescence and autophagy-induction phenotypes, and restoring H(2)S generation has been shown to eliminate the phenotypic defects of des1 mutants. Moreover, sulfide is able to reverse ATG8 accumulation and lipidation, even in wild-type plants when autophagy is induced by carbon starvation, suggesting a general effect of sulfide on autophagy regulation that is unrelated to sulfur or nitrogen limitation stress. Our results suggest that cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile of Arabidopsis. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/23144183/Cysteine_generated_sulfide_in_the_cytosol_negatively_regulates_autophagy_and_modulates_the_transcriptional_profile_in_Arabidopsis_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=23144183 DB - PRIME DP - Unbound Medicine ER -