Tags

Type your tag names separated by a space and hit enter

Hepatoprotective and cytoprotective properties of Hyptis suaveolens against oxidative stress-induced damage by CCl(4) and H(2)O(2).
Asian Pac J Trop Med. 2012 Nov; 5(11):868-74.AP

Abstract

OBJECTIVE

To investigate capacity of Hyptis suaveolens (H. suaveolens) methanol extract as an antioxidant to protect against carbon tetrachloride (CCl(4))-induced oxidative stress, hepatotoxicity in Albino Wistar rats and cytoprotective effect of hydrogen peroxide (H(2)O(2)) induced cell death in HepG(2) cell line.

METHODS

Two different doses of methanol extract of H. suaveolens were evaluated for the hepatoprotective activity against carbon tetrachloride (CCl(4)) induced hepatotoxicity in rats. Animals in Group I: served as control, group II: H. suaveolens (100 mL/kg b.w), group III: H. suaveolens (50 mL/kg b.w) + CCl(4) (1 mg/kg), group IV: H. suaveolens (100 mL/kg b.w) + CCl(4) (1 mL/kg) and group V: CCl(4) (1 mL/kg). Histopathologic changes of liver were also evaluated. Cytotoxicity was also determined by 3, (4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay.

RESULTS

Oral sigle dose treatment of CCl(4) produced a marked elevation in the serum levels of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH). Histopathological analysis of the liver of CCl(4)-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD, GSH, GST, GR and LPO. Treatment with H(2)O(2) significantly induced death of HepG(2) cell. Pretreatment with H. suaveolens methanol extract inhibited or attenuated H(2)O(2) induced cytotoxicity.

CONCLUSIONS

This study shows that H. suaveolens methanol extract can be proposed to protect the liver against CCl(4)-induced oxidative damage in rats and protect the cells against H(2)O(2)-induced oxidative damage in HepG(2) cells. The hepatoprotective and cytoprotective effects might be correlated with its antioxidant and free radical scavenger effects.

Links

FREE Publisher Full Text

Authors+Show Affiliations

Ghaffari H
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
Ghassam BJ
No affiliation info available
Prakash HS
No affiliation info available

MeSH

AnimalsAntioxidantsCarbon TetrachlorideCell LineCell SurvivalChemical and Drug Induced Liver InjuryDisease Models, AnimalHepatocytesHistocytochemistryHumansHydrogen PeroxideHyptisLiverMaleOxidative StressPlant ExtractsRatsRats, WistarStaining and LabelingTetrazolium SaltsThiazoles

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23146800

Citation

Ghaffari, Hadi, et al. "Hepatoprotective and Cytoprotective Properties of Hyptis Suaveolens Against Oxidative Stress-induced Damage By CCl(4) and H(2)O(2)." Asian Pacific Journal of Tropical Medicine, vol. 5, no. 11, 2012, pp. 868-74.
Ghaffari H, Ghassam BJ, Prakash HS. Hepatoprotective and cytoprotective properties of Hyptis suaveolens against oxidative stress-induced damage by CCl(4) and H(2)O(2). Asian Pac J Trop Med. 2012;5(11):868-74.
Ghaffari, H., Ghassam, B. J., & Prakash, H. S. (2012). Hepatoprotective and cytoprotective properties of Hyptis suaveolens against oxidative stress-induced damage by CCl(4) and H(2)O(2). Asian Pacific Journal of Tropical Medicine, 5(11), 868-74. https://doi.org/10.1016/S1995-7645(12)60162-X
Ghaffari H, Ghassam BJ, Prakash HS. Hepatoprotective and Cytoprotective Properties of Hyptis Suaveolens Against Oxidative Stress-induced Damage By CCl(4) and H(2)O(2). Asian Pac J Trop Med. 2012;5(11):868-74. PubMed PMID: 23146800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective and cytoprotective properties of Hyptis suaveolens against oxidative stress-induced damage by CCl(4) and H(2)O(2). AU - Ghaffari,Hadi, AU - Ghassam,Behrouz Jalali, AU - Prakash,H S, PY - 2012/02/24/received PY - 2012/03/31/revised PY - 2012/04/05/accepted PY - 2012/11/14/entrez PY - 2012/11/14/pubmed PY - 2013/4/16/medline SP - 868 EP - 74 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 5 IS - 11 N2 - OBJECTIVE: To investigate capacity of Hyptis suaveolens (H. suaveolens) methanol extract as an antioxidant to protect against carbon tetrachloride (CCl(4))-induced oxidative stress, hepatotoxicity in Albino Wistar rats and cytoprotective effect of hydrogen peroxide (H(2)O(2)) induced cell death in HepG(2) cell line. METHODS: Two different doses of methanol extract of H. suaveolens were evaluated for the hepatoprotective activity against carbon tetrachloride (CCl(4)) induced hepatotoxicity in rats. Animals in Group I: served as control, group II: H. suaveolens (100 mL/kg b.w), group III: H. suaveolens (50 mL/kg b.w) + CCl(4) (1 mg/kg), group IV: H. suaveolens (100 mL/kg b.w) + CCl(4) (1 mL/kg) and group V: CCl(4) (1 mL/kg). Histopathologic changes of liver were also evaluated. Cytotoxicity was also determined by 3, (4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. RESULTS: Oral sigle dose treatment of CCl(4) produced a marked elevation in the serum levels of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH). Histopathological analysis of the liver of CCl(4)-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD, GSH, GST, GR and LPO. Treatment with H(2)O(2) significantly induced death of HepG(2) cell. Pretreatment with H. suaveolens methanol extract inhibited or attenuated H(2)O(2) induced cytotoxicity. CONCLUSIONS: This study shows that H. suaveolens methanol extract can be proposed to protect the liver against CCl(4)-induced oxidative damage in rats and protect the cells against H(2)O(2)-induced oxidative damage in HepG(2) cells. The hepatoprotective and cytoprotective effects might be correlated with its antioxidant and free radical scavenger effects. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/23146800/Hepatoprotective_and_cytoprotective_properties_of_Hyptis_suaveolens_against_oxidative_stress_induced_damage_by_CCl_4__and_H_2_O_2__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(12)60162-X DB - PRIME DP - Unbound Medicine ER -