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Identification and characterization of an epi-allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice.
Plant Cell. 2012 Nov; 24(11):4407-21.PC

Abstract

DNA methylation and histone H3 Lys 9 dimethylation (H3K9me2) are important epigenetic repression marks for silencing transposons in heterochromatin and for regulating gene expression. However, the mechanistic relationship to other repressive marks, such as histone H3 Lys 27 trimethylation (H3K27me3) is unclear. FERTILIZATION-INDEPENDENT ENDOSPERM1 (FIE1) encodes an Esc-like core component of the Polycomb repressive complex 2, which is involved in H3K27me3-mediated gene repression. Here, we identify a gain-of-function epi-allele (Epi-df) of rice (Oryza sativa) FIE1; this allele causes a dwarf stature and various floral defects that are inherited in a dominant fashion. We found that Epi-df has no changes in nucleotide sequence but is hypomethylated in the 5' region of FIE1 and has reduced H3K9me2 and increased H3K4me3. In Epi-df, FIE1 was ectopically expressed and its imprinting was disrupted. FIE1 interacted with rice Enhancer of Zeste homologs, consistent with its role in H3K27me3 repression. Ectopic expression of FIE1 in Epi-df resulted in alteration of H3K27me3 levels in hundreds of genes. In summary, this work identifies an epi-allele involved in H3K27me3-mediated gene repression that itself is highly regulated by DNA methylation and histone H3K9me2, thereby shedding light on the link between DNA methylation and histone methylation, the two important epigenetic marks regulating rice development.

Authors+Show Affiliations

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23150632

Citation

Zhang, Liguo, et al. "Identification and Characterization of an Epi-allele of FIE1 Reveals a Regulatory Linkage Between Two Epigenetic Marks in Rice." The Plant Cell, vol. 24, no. 11, 2012, pp. 4407-21.
Zhang L, Cheng Z, Qin R, et al. Identification and characterization of an epi-allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice. Plant Cell. 2012;24(11):4407-21.
Zhang, L., Cheng, Z., Qin, R., Qiu, Y., Wang, J. L., Cui, X., Gu, L., Zhang, X., Guo, X., Wang, D., Jiang, L., Wu, C. Y., Wang, H., Cao, X., & Wan, J. (2012). Identification and characterization of an epi-allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice. The Plant Cell, 24(11), 4407-21. https://doi.org/10.1105/tpc.112.102269
Zhang L, et al. Identification and Characterization of an Epi-allele of FIE1 Reveals a Regulatory Linkage Between Two Epigenetic Marks in Rice. Plant Cell. 2012;24(11):4407-21. PubMed PMID: 23150632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of an epi-allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice. AU - Zhang,Liguo, AU - Cheng,Zhijun, AU - Qin,Ruizhen, AU - Qiu,Yang, AU - Wang,Jiu-Lin, AU - Cui,Xiekui, AU - Gu,Lianfeng, AU - Zhang,Xin, AU - Guo,Xiuping, AU - Wang,Dan, AU - Jiang,Ling, AU - Wu,Chuan-yin, AU - Wang,Haiyang, AU - Cao,Xiaofeng, AU - Wan,Jianmin, Y1 - 2012/11/13/ PY - 2012/11/15/entrez PY - 2012/11/15/pubmed PY - 2013/8/22/medline SP - 4407 EP - 21 JF - The Plant cell JO - Plant Cell VL - 24 IS - 11 N2 - DNA methylation and histone H3 Lys 9 dimethylation (H3K9me2) are important epigenetic repression marks for silencing transposons in heterochromatin and for regulating gene expression. However, the mechanistic relationship to other repressive marks, such as histone H3 Lys 27 trimethylation (H3K27me3) is unclear. FERTILIZATION-INDEPENDENT ENDOSPERM1 (FIE1) encodes an Esc-like core component of the Polycomb repressive complex 2, which is involved in H3K27me3-mediated gene repression. Here, we identify a gain-of-function epi-allele (Epi-df) of rice (Oryza sativa) FIE1; this allele causes a dwarf stature and various floral defects that are inherited in a dominant fashion. We found that Epi-df has no changes in nucleotide sequence but is hypomethylated in the 5' region of FIE1 and has reduced H3K9me2 and increased H3K4me3. In Epi-df, FIE1 was ectopically expressed and its imprinting was disrupted. FIE1 interacted with rice Enhancer of Zeste homologs, consistent with its role in H3K27me3 repression. Ectopic expression of FIE1 in Epi-df resulted in alteration of H3K27me3 levels in hundreds of genes. In summary, this work identifies an epi-allele involved in H3K27me3-mediated gene repression that itself is highly regulated by DNA methylation and histone H3K9me2, thereby shedding light on the link between DNA methylation and histone methylation, the two important epigenetic marks regulating rice development. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/23150632/Identification_and_characterization_of_an_epi_allele_of_FIE1_reveals_a_regulatory_linkage_between_two_epigenetic_marks_in_rice_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=23150632 DB - PRIME DP - Unbound Medicine ER -