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FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe.
Plant Cell. 2012 Nov; 24(11):4539-54.PC

Abstract

An actin fringe structure in the subapex plays an important role in pollen tube tip growth. However, the precise mechanism by which the actin fringe is generated and maintained remains largely unknown. Here, we cloned a 2606-bp full-length cDNA encoding a deduced 77-kD fimbrin-like protein from lily (Lilium longiflorum), named FIMBRIN1 (FIM1). Ll-FIM1 was preferentially expressed in pollen and concentrated at actin fringe in the subapical region, as well as in longitudinal actin-filament bundles in the shank of pollen tubes. Microinjection of Ll-FIM1 antibody into lily pollen tubes inhibited tip growth and disrupted the actin fringe. Furthermore, we verified the function of Ll-FIM1 in the fim5 mutant of its closest relative, Arabidopsis thaliana. Pollen tubes of fim5 mutants grew with a larger diameter in early stages but could recover into normal forms in later stages, despite significantly slower growth rates. The actin fringe of the fim5 mutants, however, was impaired during both early and late stages. Impressively, stable expression of fim5pro:GFP:Ll-FIM1 rescued the actin fringe and the growth rate of Arabidopsis fim5 pollen tubes. In vitro biochemical analysis showed that Ll-FIM1 could bundle actin filaments. Thus, our study has identified a fimbrin that may stabilize the actin fringe by cross-linking actin filaments into bundles, which is important for proper tip growth of lily pollen tubes.

Authors+Show Affiliations

Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23150633

Citation

Su, Hui, et al. "FIMBRIN1 Is Involved in Lily Pollen Tube Growth By Stabilizing the Actin Fringe." The Plant Cell, vol. 24, no. 11, 2012, pp. 4539-54.
Su H, Zhu J, Cai C, et al. FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe. Plant Cell. 2012;24(11):4539-54.
Su, H., Zhu, J., Cai, C., Pei, W., Wang, J., Dong, H., & Ren, H. (2012). FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe. The Plant Cell, 24(11), 4539-54. https://doi.org/10.1105/tpc.112.099358
Su H, et al. FIMBRIN1 Is Involved in Lily Pollen Tube Growth By Stabilizing the Actin Fringe. Plant Cell. 2012;24(11):4539-54. PubMed PMID: 23150633.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe. AU - Su,Hui, AU - Zhu,Jinsheng, AU - Cai,Chao, AU - Pei,Weike, AU - Wang,Jiaojiao, AU - Dong,Huaijian, AU - Ren,Haiyun, Y1 - 2012/11/13/ PY - 2012/11/15/entrez PY - 2012/11/15/pubmed PY - 2013/8/22/medline SP - 4539 EP - 54 JF - The Plant cell JO - Plant Cell VL - 24 IS - 11 N2 - An actin fringe structure in the subapex plays an important role in pollen tube tip growth. However, the precise mechanism by which the actin fringe is generated and maintained remains largely unknown. Here, we cloned a 2606-bp full-length cDNA encoding a deduced 77-kD fimbrin-like protein from lily (Lilium longiflorum), named FIMBRIN1 (FIM1). Ll-FIM1 was preferentially expressed in pollen and concentrated at actin fringe in the subapical region, as well as in longitudinal actin-filament bundles in the shank of pollen tubes. Microinjection of Ll-FIM1 antibody into lily pollen tubes inhibited tip growth and disrupted the actin fringe. Furthermore, we verified the function of Ll-FIM1 in the fim5 mutant of its closest relative, Arabidopsis thaliana. Pollen tubes of fim5 mutants grew with a larger diameter in early stages but could recover into normal forms in later stages, despite significantly slower growth rates. The actin fringe of the fim5 mutants, however, was impaired during both early and late stages. Impressively, stable expression of fim5pro:GFP:Ll-FIM1 rescued the actin fringe and the growth rate of Arabidopsis fim5 pollen tubes. In vitro biochemical analysis showed that Ll-FIM1 could bundle actin filaments. Thus, our study has identified a fimbrin that may stabilize the actin fringe by cross-linking actin filaments into bundles, which is important for proper tip growth of lily pollen tubes. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/23150633/FIMBRIN1_is_involved_in_lily_pollen_tube_growth_by_stabilizing_the_actin_fringe_ L2 - https://academic.oup.com/plcell/article-lookup/doi/10.1105/tpc.112.099358 DB - PRIME DP - Unbound Medicine ER -