Tags

Type your tag names separated by a space and hit enter

Neuroprotective and antioxidant potential of terpenoid fraction from Hygrophila auriculata against transient global cerebral ischemia in rats.
Pharm Biol. 2013 Feb; 51(2):181-9.PB

Abstract

CONTEXT

The plant Hygrophila auriculata (K. Schum) Heine. (Acanthaceae) is widely used in the Indian System of Medicine as "Rasayana" for treating brain and liver diseases.

OBJECTIVES

The present study evaluated the in vivo antioxidant and neuroprotective effect of aterpenoid rich fraction (TF) from Hygrophila auriculata in a rat model of transient global cerebral ischemia (tGCI).

MATERIALS AND METHODS

Male Wistar rats were grouped as sham control, tGCI control, vitamin E (500 mg/kg) and TF (100 & 200 mg/kg) treated groups. Following 7 days of drug administration, animals were subjected to tGCI by permanent occlusion of both vertebral and transient occlusion of carotid arteries for 10 min followed by reperfusion. The neuroprotective effect was assessed by tGCI induced neurological, sensory motor deficit in rats. Brain antioxidants such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were investigated. Further, a histopathological examination was done in CA1 hippocampus.

RESULTS

tGCI induction resulted in an increase in beam balance score (5.1), number of entries in open field (131) and a decrease in time spent in rotorod (47 s). In contrast, TF treatment resulted in a significant decrease in (p < 0.01) beam balance score (2.9), number of entries (67) and increased time spent in rotorod (63.25 s). There was also a significant (p < 0.001) decrease in brain SOD and GSH with an increase in MDA. TF treatment resulted in restoration of antioxidants and protection of hippocampal CA1 neurons against tGCI insult.

CONCLUSION

It is concluded that TF from Hygrophila auriculata shows neuroprotective potential against tGCI induced oxidative stress.

Authors+Show Affiliations

Department of Pharmacology, Vels Institute of Science Technology and Advanced Studies, School of Pharmaceutical Sciences, Vels University, Chennai, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23153190

Citation

Kanhere, Rupesh, et al. "Neuroprotective and Antioxidant Potential of Terpenoid Fraction From Hygrophila Auriculata Against Transient Global Cerebral Ischemia in Rats." Pharmaceutical Biology, vol. 51, no. 2, 2013, pp. 181-9.
Kanhere R, Anjana A, Anbu J, et al. Neuroprotective and antioxidant potential of terpenoid fraction from Hygrophila auriculata against transient global cerebral ischemia in rats. Pharm Biol. 2013;51(2):181-9.
Kanhere, R., Anjana, A., Anbu, J., Sumithra, M., & K F H, N. A. (2013). Neuroprotective and antioxidant potential of terpenoid fraction from Hygrophila auriculata against transient global cerebral ischemia in rats. Pharmaceutical Biology, 51(2), 181-9. https://doi.org/10.3109/13880209.2012.716851
Kanhere R, et al. Neuroprotective and Antioxidant Potential of Terpenoid Fraction From Hygrophila Auriculata Against Transient Global Cerebral Ischemia in Rats. Pharm Biol. 2013;51(2):181-9. PubMed PMID: 23153190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective and antioxidant potential of terpenoid fraction from Hygrophila auriculata against transient global cerebral ischemia in rats. AU - Kanhere,Rupesh, AU - Anjana,Ashwini, AU - Anbu,Jayaraman, AU - Sumithra,Mohan, AU - K F H,Nazeer Ahamed, Y1 - 2012/11/16/ PY - 2012/11/17/entrez PY - 2012/11/17/pubmed PY - 2013/6/29/medline SP - 181 EP - 9 JF - Pharmaceutical biology JO - Pharm Biol VL - 51 IS - 2 N2 - CONTEXT: The plant Hygrophila auriculata (K. Schum) Heine. (Acanthaceae) is widely used in the Indian System of Medicine as "Rasayana" for treating brain and liver diseases. OBJECTIVES: The present study evaluated the in vivo antioxidant and neuroprotective effect of aterpenoid rich fraction (TF) from Hygrophila auriculata in a rat model of transient global cerebral ischemia (tGCI). MATERIALS AND METHODS: Male Wistar rats were grouped as sham control, tGCI control, vitamin E (500 mg/kg) and TF (100 & 200 mg/kg) treated groups. Following 7 days of drug administration, animals were subjected to tGCI by permanent occlusion of both vertebral and transient occlusion of carotid arteries for 10 min followed by reperfusion. The neuroprotective effect was assessed by tGCI induced neurological, sensory motor deficit in rats. Brain antioxidants such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were investigated. Further, a histopathological examination was done in CA1 hippocampus. RESULTS: tGCI induction resulted in an increase in beam balance score (5.1), number of entries in open field (131) and a decrease in time spent in rotorod (47 s). In contrast, TF treatment resulted in a significant decrease in (p < 0.01) beam balance score (2.9), number of entries (67) and increased time spent in rotorod (63.25 s). There was also a significant (p < 0.001) decrease in brain SOD and GSH with an increase in MDA. TF treatment resulted in restoration of antioxidants and protection of hippocampal CA1 neurons against tGCI insult. CONCLUSION: It is concluded that TF from Hygrophila auriculata shows neuroprotective potential against tGCI induced oxidative stress. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/23153190/Neuroprotective_and_antioxidant_potential_of_terpenoid_fraction_from_Hygrophila_auriculata_against_transient_global_cerebral_ischemia_in_rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2012.716851 DB - PRIME DP - Unbound Medicine ER -