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Molecular evidence for the occurrence of Japanese encephalitis virus genotype I and III infection associated with acute encephalitis in patients of West Bengal, India, 2010.
Virol J. 2012 Nov 15; 9:271.VJ

Abstract

BACKGROUND

Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is the sole etiologic agent of Japanese Encephalitis (JE); a neurotropic killer disease which is one of the major causes of viral encephalitis worldwide with prime public health concern. JE was first reported in the state of West Bengal, India in 1973. Since then it is being reported every year from different districts of the state, though the vaccination has already been done. Therefore, it indicates that there might be either partial coverage of the vaccine or the emergence of mutated/new strain of JEV. Considering this fact, to understand the JEV genotype distribution, we conducted a molecular epidemiological study on a total of 135 serum/cerebrospinal fluid (CSF) samples referred and/or collected from the clinically suspected patients with Acute encephalitis syndrome (AES), admitted in different district hospitals of West Bengal, India, 2010.

FINDINGS

JEV etiology was confirmed in 36/135 (26.6%) and 13/61 (21.3%) 2-15 days' febrile illness samples from AES cases by analyzing Mac-ELISA followed by RT-PCR test respectively. Phylogenetic analysis based on complete envelope gene sequences of 13 isolates showed the emergence of JEV genotype I (GI), co-circulating with genotype III (GIII).

CONCLUSION

This study represents the first report of JEV GI with GIII, co-circulating in West Bengal. The efficacy of the vaccine (derived from JEV GIII strain SA-14-14-2) to protect against emerging JEV GI needs careful evaluation. In future, JE outbreak is quite likely in the state, if this vaccine fails to protect sufficiently against GI of JEV.

Authors+Show Affiliations

ICMR virus unit, ID & BG Hospital, 57, Dr, S, C, Banerjee Road, Beliaghata, Kolkata-700010, West Bengal, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23153306

Citation

Sarkar, Arindam, et al. "Molecular Evidence for the Occurrence of Japanese Encephalitis Virus Genotype I and III Infection Associated With Acute Encephalitis in Patients of West Bengal, India, 2010." Virology Journal, vol. 9, 2012, p. 271.
Sarkar A, Taraphdar D, Mukhopadhyay SK, et al. Molecular evidence for the occurrence of Japanese encephalitis virus genotype I and III infection associated with acute encephalitis in patients of West Bengal, India, 2010. Virol J. 2012;9:271.
Sarkar, A., Taraphdar, D., Mukhopadhyay, S. K., Chakrabarti, S., & Chatterjee, S. (2012). Molecular evidence for the occurrence of Japanese encephalitis virus genotype I and III infection associated with acute encephalitis in patients of West Bengal, India, 2010. Virology Journal, 9, 271. https://doi.org/10.1186/1743-422X-9-271
Sarkar A, et al. Molecular Evidence for the Occurrence of Japanese Encephalitis Virus Genotype I and III Infection Associated With Acute Encephalitis in Patients of West Bengal, India, 2010. Virol J. 2012 Nov 15;9:271. PubMed PMID: 23153306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular evidence for the occurrence of Japanese encephalitis virus genotype I and III infection associated with acute encephalitis in patients of West Bengal, India, 2010. AU - Sarkar,Arindam, AU - Taraphdar,Debjani, AU - Mukhopadhyay,Subhra Kanti, AU - Chakrabarti,Sekhar, AU - Chatterjee,Shyamalendu, Y1 - 2012/11/15/ PY - 2012/02/03/received PY - 2012/10/23/accepted PY - 2012/11/17/entrez PY - 2012/11/17/pubmed PY - 2013/7/3/medline SP - 271 EP - 271 JF - Virology journal JO - Virol J VL - 9 N2 - BACKGROUND: Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is the sole etiologic agent of Japanese Encephalitis (JE); a neurotropic killer disease which is one of the major causes of viral encephalitis worldwide with prime public health concern. JE was first reported in the state of West Bengal, India in 1973. Since then it is being reported every year from different districts of the state, though the vaccination has already been done. Therefore, it indicates that there might be either partial coverage of the vaccine or the emergence of mutated/new strain of JEV. Considering this fact, to understand the JEV genotype distribution, we conducted a molecular epidemiological study on a total of 135 serum/cerebrospinal fluid (CSF) samples referred and/or collected from the clinically suspected patients with Acute encephalitis syndrome (AES), admitted in different district hospitals of West Bengal, India, 2010. FINDINGS: JEV etiology was confirmed in 36/135 (26.6%) and 13/61 (21.3%) 2-15 days' febrile illness samples from AES cases by analyzing Mac-ELISA followed by RT-PCR test respectively. Phylogenetic analysis based on complete envelope gene sequences of 13 isolates showed the emergence of JEV genotype I (GI), co-circulating with genotype III (GIII). CONCLUSION: This study represents the first report of JEV GI with GIII, co-circulating in West Bengal. The efficacy of the vaccine (derived from JEV GIII strain SA-14-14-2) to protect against emerging JEV GI needs careful evaluation. In future, JE outbreak is quite likely in the state, if this vaccine fails to protect sufficiently against GI of JEV. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/23153306/Molecular_evidence_for_the_occurrence_of_Japanese_encephalitis_virus_genotype_I_and_III_infection_associated_with_acute_encephalitis_in_patients_of_West_Bengal_India_2010_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-271 DB - PRIME DP - Unbound Medicine ER -