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A new low-cost procedure for detecting nucleic acids in low-incidence samples: a case study of detecting spores of Paenibacillus larvae from bee debris.
J Econ Entomol. 2012 Oct; 105(5):1487-91.JE

Abstract

American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less).

Authors+Show Affiliations

Department of Zoology, Faculty of Science, Charles University in Prague, Prague, Czech Republic. stepan.ryba@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23156141

Citation

Ryba, Stepan, et al. "A New Low-cost Procedure for Detecting Nucleic Acids in Low-incidence Samples: a Case Study of Detecting Spores of Paenibacillus Larvae From Bee Debris." Journal of Economic Entomology, vol. 105, no. 5, 2012, pp. 1487-91.
Ryba S, Kindlmann P, Titera D, et al. A new low-cost procedure for detecting nucleic acids in low-incidence samples: a case study of detecting spores of Paenibacillus larvae from bee debris. J Econ Entomol. 2012;105(5):1487-91.
Ryba, S., Kindlmann, P., Titera, D., Haklova, M., & Stopka, P. (2012). A new low-cost procedure for detecting nucleic acids in low-incidence samples: a case study of detecting spores of Paenibacillus larvae from bee debris. Journal of Economic Entomology, 105(5), 1487-91.
Ryba S, et al. A New Low-cost Procedure for Detecting Nucleic Acids in Low-incidence Samples: a Case Study of Detecting Spores of Paenibacillus Larvae From Bee Debris. J Econ Entomol. 2012;105(5):1487-91. PubMed PMID: 23156141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new low-cost procedure for detecting nucleic acids in low-incidence samples: a case study of detecting spores of Paenibacillus larvae from bee debris. AU - Ryba,Stepan, AU - Kindlmann,Pavel, AU - Titera,Dalibor, AU - Haklova,Marcela, AU - Stopka,Pavel, PY - 2012/11/20/entrez PY - 2012/11/20/pubmed PY - 2012/12/10/medline SP - 1487 EP - 91 JF - Journal of economic entomology JO - J. Econ. Entomol. VL - 105 IS - 5 N2 - American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use the new method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less). SN - 0022-0493 UR - https://www.unboundmedicine.com/medline/citation/23156141/A_new_low_cost_procedure_for_detecting_nucleic_acids_in_low_incidence_samples:_a_case_study_of_detecting_spores_of_Paenibacillus_larvae_from_bee_debris_ L2 - https://academic.oup.com/jee/article-lookup/doi/10.1603/ec12010 DB - PRIME DP - Unbound Medicine ER -