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Identification of differentially expressed genes and pathways between primary osteoarthritis and endemic osteoarthritis (Kashin-Beck disease).
Scand J Rheumatol. 2013; 42(1):71-9.SJ

Abstract

OBJECTIVES

Primary osteoarthritis (OA) and Kashin-Beck disease (KBD) exhibit similar clinical manifestations and common articular cartilage lesions. Revealing the pathogenetic differences between OA and KBD is helpful for differential diagnosis and may provide new insights into the pathogenesis of OA and KBD. In this study, we compared the genome-wide gene ontology (GO) and pathway expression patterns of articular cartilage derived from both OA and KBD patients.

METHODS

Total RNA was isolated, amplified, labelled, and hybridized using Agilent whole genome microarray analysis. Gene set enrichment analysis (GSEA) was used to identify differentially expressed genes and pathways between OA and KBD. Nine differentially expressed GO categories and 85 differentially expressed pathways were identified by this study.

RESULTS

The reactive oxygen species (ROS)-related HOUSTIS_ROS pathway and the vascular endothelial growth factor (VEGF)-related ABE_VEGFA_TARGETS_2HR pathway were significantly up-regulated in OA compared to KBD. Higher expression levels of the collagen-related COLLAGEN GO, EXTRACELLULAR_MATRIX_PART GO, and nitric oxide (NO)-related BIOCARTA_NO1_PATHWAY pathways were detected in KBD than in OA.

CONCLUSIONS

ROS-induced cartilage lesions seem to be more involved in the pathogenesis of OA whereas NO-mediated chondrocyte apoptosis contributes more to the development of KBD.

Authors+Show Affiliations

Key Laboratory of Environment and Gene Related Diseases, Ministry of Education, Faculty of Public Health, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P R China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23157206

Citation

Zhang, F, et al. "Identification of Differentially Expressed Genes and Pathways Between Primary Osteoarthritis and Endemic Osteoarthritis (Kashin-Beck Disease)." Scandinavian Journal of Rheumatology, vol. 42, no. 1, 2013, pp. 71-9.
Zhang F, Guo X, Duan C, et al. Identification of differentially expressed genes and pathways between primary osteoarthritis and endemic osteoarthritis (Kashin-Beck disease). Scand J Rheumatol. 2013;42(1):71-9.
Zhang, F., Guo, X., Duan, C., Wu, S., Yu, H., & Lammi, M. (2013). Identification of differentially expressed genes and pathways between primary osteoarthritis and endemic osteoarthritis (Kashin-Beck disease). Scandinavian Journal of Rheumatology, 42(1), 71-9. https://doi.org/10.3109/03009742.2012.698303
Zhang F, et al. Identification of Differentially Expressed Genes and Pathways Between Primary Osteoarthritis and Endemic Osteoarthritis (Kashin-Beck Disease). Scand J Rheumatol. 2013;42(1):71-9. PubMed PMID: 23157206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of differentially expressed genes and pathways between primary osteoarthritis and endemic osteoarthritis (Kashin-Beck disease). AU - Zhang,F, AU - Guo,X, AU - Duan,C, AU - Wu,S, AU - Yu,H, AU - Lammi,M, Y1 - 2012/11/17/ PY - 2012/11/20/entrez PY - 2012/11/20/pubmed PY - 2013/3/21/medline SP - 71 EP - 9 JF - Scandinavian journal of rheumatology JO - Scand. J. Rheumatol. VL - 42 IS - 1 N2 - OBJECTIVES: Primary osteoarthritis (OA) and Kashin-Beck disease (KBD) exhibit similar clinical manifestations and common articular cartilage lesions. Revealing the pathogenetic differences between OA and KBD is helpful for differential diagnosis and may provide new insights into the pathogenesis of OA and KBD. In this study, we compared the genome-wide gene ontology (GO) and pathway expression patterns of articular cartilage derived from both OA and KBD patients. METHODS: Total RNA was isolated, amplified, labelled, and hybridized using Agilent whole genome microarray analysis. Gene set enrichment analysis (GSEA) was used to identify differentially expressed genes and pathways between OA and KBD. Nine differentially expressed GO categories and 85 differentially expressed pathways were identified by this study. RESULTS: The reactive oxygen species (ROS)-related HOUSTIS_ROS pathway and the vascular endothelial growth factor (VEGF)-related ABE_VEGFA_TARGETS_2HR pathway were significantly up-regulated in OA compared to KBD. Higher expression levels of the collagen-related COLLAGEN GO, EXTRACELLULAR_MATRIX_PART GO, and nitric oxide (NO)-related BIOCARTA_NO1_PATHWAY pathways were detected in KBD than in OA. CONCLUSIONS: ROS-induced cartilage lesions seem to be more involved in the pathogenesis of OA whereas NO-mediated chondrocyte apoptosis contributes more to the development of KBD. SN - 1502-7732 UR - https://www.unboundmedicine.com/medline/citation/23157206/Identification_of_differentially_expressed_genes_and_pathways_between_primary_osteoarthritis_and_endemic_osteoarthritis__Kashin_Beck_disease__ L2 - http://www.tandfonline.com/doi/full/10.3109/03009742.2012.698303 DB - PRIME DP - Unbound Medicine ER -