TY - JOUR T1 - Development and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines. AU - Paula Barros,Elisabete, AU - Moreira,Nathalie, AU - Elias Pereira,Giuliano, AU - Leite,Selma Gomes Ferreira, AU - Moraes Rezende,Claudia, AU - Guedes de Pinho,Paula, Y1 - 2012/09/08/ PY - 2012/04/12/received PY - 2012/08/21/revised PY - 2012/08/22/accepted PY - 2012/11/20/entrez PY - 2012/11/20/pubmed PY - 2013/5/2/medline SP - 177 EP - 86 JF - Talanta JO - Talanta VL - 101 N2 - An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, norisoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time, and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5 ml of wine with 2g NaCl at 45 °C during 5 min, and subsequent extraction of 30 min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile composition of different white wines. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/23158309/Development_and_validation_of_automatic_HS_SPME_with_a_gas_chromatography_ion_trap/mass_spectrometry_method_for_analysis_of_volatiles_in_wines_ DB - PRIME DP - Unbound Medicine ER -