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In vivo and in vitro evaluation of the use of a newly developed melatonin loaded emulsion combined with UV filters as a protective agent against skin irradiation.
J Dermatol Sci. 2013 Mar; 69(3):202-14.JD

Abstract

BACKGROUND

Melatonin has attracted attention because of their high antioxidant and anticarcinogenic activity. Otherwise, the use of sunscreens is recommended for patients after chemotherapy and radiotherapy treatments or to prevent UV radiation-induced skin damages that may result in pre-cancerous and cancerous skin lesions.

OBJECTIVE

To evaluate the beneficial influence of melatonin in topical sunscreen emulsions combined with three common ultraviolet filters.

METHODS

After the formulation characterization in terms of rheology, stability studies were performed. Release studies let us to evaluate its mechanism of delivery and ex vivo permeation study through human skin, the amount of melatonin retained. The antioxidant activity assay was also carried out, and finally the in vivo photoprotective effect in rats was tested as transepidermal water loss and erythema formation.

RESULTS

The rheological behaviour of formulations was pseudoplastic fluid, all emulsions had good physical stability. Release studies showed a trend of enhancement in melatonin release from emulsions incorporating UV filters and followed a Weibull model. Melatonin permeation was higher from the emulsion containing melatonin combined with a mixture of three ultraviolet filters (MMIX) formulation. Equally this formulation exhibited the highest radical scavenging activity. Finally the photoprotective assay showed that only skin areas treated with this formulation were statistically equivalent to the unirradiated control area.

CONCLUSION

MMIX formulation would be a promising formulation for preventing the undesirable adverse effects of UV skin irradiation because melatonin not only acts as a potent antioxidant itself, but also is capable of activating an endogenous enzymatic protective system against oxidative stress.

Authors+Show Affiliations

Pharmacy and Pharmaceutical Technology Department, Faculty of Pharmacy, University of Barcelona, Joan XXIII avenue, 08028 Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23159282

Citation

Sierra, Ana Flo, et al. "In Vivo and in Vitro Evaluation of the Use of a Newly Developed Melatonin Loaded Emulsion Combined With UV Filters as a Protective Agent Against Skin Irradiation." Journal of Dermatological Science, vol. 69, no. 3, 2013, pp. 202-14.
Sierra AF, Ramírez ML, Campmany AC, et al. In vivo and in vitro evaluation of the use of a newly developed melatonin loaded emulsion combined with UV filters as a protective agent against skin irradiation. J Dermatol Sci. 2013;69(3):202-14.
Sierra, A. F., Ramírez, M. L., Campmany, A. C., Martínez, A. R., & Naveros, B. C. (2013). In vivo and in vitro evaluation of the use of a newly developed melatonin loaded emulsion combined with UV filters as a protective agent against skin irradiation. Journal of Dermatological Science, 69(3), 202-14. https://doi.org/10.1016/j.jdermsci.2012.10.013
Sierra AF, et al. In Vivo and in Vitro Evaluation of the Use of a Newly Developed Melatonin Loaded Emulsion Combined With UV Filters as a Protective Agent Against Skin Irradiation. J Dermatol Sci. 2013;69(3):202-14. PubMed PMID: 23159282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo and in vitro evaluation of the use of a newly developed melatonin loaded emulsion combined with UV filters as a protective agent against skin irradiation. AU - Sierra,Ana Flo, AU - Ramírez,Maria L Garduño, AU - Campmany,Ana C Calpena, AU - Martínez,Adolfina Ruiz, AU - Naveros,Beatriz Clares, Y1 - 2012/10/30/ PY - 2012/08/14/received PY - 2012/10/18/revised PY - 2012/10/22/accepted PY - 2012/11/20/entrez PY - 2012/11/20/pubmed PY - 2013/9/24/medline SP - 202 EP - 14 JF - Journal of dermatological science JO - J Dermatol Sci VL - 69 IS - 3 N2 - BACKGROUND: Melatonin has attracted attention because of their high antioxidant and anticarcinogenic activity. Otherwise, the use of sunscreens is recommended for patients after chemotherapy and radiotherapy treatments or to prevent UV radiation-induced skin damages that may result in pre-cancerous and cancerous skin lesions. OBJECTIVE: To evaluate the beneficial influence of melatonin in topical sunscreen emulsions combined with three common ultraviolet filters. METHODS: After the formulation characterization in terms of rheology, stability studies were performed. Release studies let us to evaluate its mechanism of delivery and ex vivo permeation study through human skin, the amount of melatonin retained. The antioxidant activity assay was also carried out, and finally the in vivo photoprotective effect in rats was tested as transepidermal water loss and erythema formation. RESULTS: The rheological behaviour of formulations was pseudoplastic fluid, all emulsions had good physical stability. Release studies showed a trend of enhancement in melatonin release from emulsions incorporating UV filters and followed a Weibull model. Melatonin permeation was higher from the emulsion containing melatonin combined with a mixture of three ultraviolet filters (MMIX) formulation. Equally this formulation exhibited the highest radical scavenging activity. Finally the photoprotective assay showed that only skin areas treated with this formulation were statistically equivalent to the unirradiated control area. CONCLUSION: MMIX formulation would be a promising formulation for preventing the undesirable adverse effects of UV skin irradiation because melatonin not only acts as a potent antioxidant itself, but also is capable of activating an endogenous enzymatic protective system against oxidative stress. SN - 1873-569X UR - https://www.unboundmedicine.com/medline/citation/23159282/In_vivo_and_in_vitro_evaluation_of_the_use_of_a_newly_developed_melatonin_loaded_emulsion_combined_with_UV_filters_as_a_protective_agent_against_skin_irradiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-1811(12)00323-4 DB - PRIME DP - Unbound Medicine ER -