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Human amniotic fluid stem cell-derived muscle progenitor cell therapy for stress urinary incontinence.
J Korean Med Sci. 2012 Nov; 27(11):1300-7.JK

Abstract

The most promising treatment for stress urinary incontinence can be a cell therapy. We suggest human amniotic fluid stem cells (hAFSCs) as an alternative cell source. We established the optimum in vitro protocol for the differentiation from hAFSCs into muscle progenitors. These progenitors were transplanted into the injured urethral sphincter and their therapeutic effect was analyzed. For the development of an efficient differentiation system in vitro, we examined a commercial medium, co-culture and conditioned medium (CM) systems. After being treated with CM, hAFSCs were effectively developed into a muscle lineage. The progenitors were integrated into the host urethral sphincter and the host cell differentiation was stimulated in vivo. Urodynamic analysis showed significant increase of leak point pressure and closing pressure. Immunohistochemistry revealed the regeneration of circular muscle mass with normal appearance. Molecular analysis observed the expression of a larger number of target markers. In the immunogenicity analysis, the progenitor group had a scant CD8 lymphocyte. In tumorigenicity, the progenitors showed no teratoma formation. These results suggest that hAFSCs can effectively be differentiated into muscle progenitors in CM and that the hAFSC-derived muscle progenitors are an accessible cell source for the regeneration of injured urethral sphincter.

Authors+Show Affiliations

Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23166409

Citation

Chun, So Young, et al. "Human Amniotic Fluid Stem Cell-derived Muscle Progenitor Cell Therapy for Stress Urinary Incontinence." Journal of Korean Medical Science, vol. 27, no. 11, 2012, pp. 1300-7.
Chun SY, Cho DH, Chae SY, et al. Human amniotic fluid stem cell-derived muscle progenitor cell therapy for stress urinary incontinence. J Korean Med Sci. 2012;27(11):1300-7.
Chun, S. Y., Cho, D. H., Chae, S. Y., Choi, K. H., Lim, H. J., Yoon, G. S., Kim, B. S., Kim, B. W., Yoo, J. J., & Kwon, T. G. (2012). Human amniotic fluid stem cell-derived muscle progenitor cell therapy for stress urinary incontinence. Journal of Korean Medical Science, 27(11), 1300-7. https://doi.org/10.3346/jkms.2012.27.11.1300
Chun SY, et al. Human Amniotic Fluid Stem Cell-derived Muscle Progenitor Cell Therapy for Stress Urinary Incontinence. J Korean Med Sci. 2012;27(11):1300-7. PubMed PMID: 23166409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human amniotic fluid stem cell-derived muscle progenitor cell therapy for stress urinary incontinence. AU - Chun,So Young, AU - Cho,Deok Hyun, AU - Chae,Seon Yeong, AU - Choi,Kyung Hee, AU - Lim,Hyun Ju, AU - Yoon,Ghil Suk, AU - Kim,Bum Soo, AU - Kim,Bup Wan, AU - Yoo,James J, AU - Kwon,Tae Gyun, Y1 - 2012/10/30/ PY - 2012/04/24/received PY - 2012/08/10/accepted PY - 2012/11/21/entrez PY - 2012/11/21/pubmed PY - 2013/4/27/medline KW - Amniotic Fluid KW - Cell Differentiation KW - Regeneration KW - Stem Cells KW - Urinary Incontinence KW - Urinary Sphincter SP - 1300 EP - 7 JF - Journal of Korean medical science JO - J. Korean Med. Sci. VL - 27 IS - 11 N2 - The most promising treatment for stress urinary incontinence can be a cell therapy. We suggest human amniotic fluid stem cells (hAFSCs) as an alternative cell source. We established the optimum in vitro protocol for the differentiation from hAFSCs into muscle progenitors. These progenitors were transplanted into the injured urethral sphincter and their therapeutic effect was analyzed. For the development of an efficient differentiation system in vitro, we examined a commercial medium, co-culture and conditioned medium (CM) systems. After being treated with CM, hAFSCs were effectively developed into a muscle lineage. The progenitors were integrated into the host urethral sphincter and the host cell differentiation was stimulated in vivo. Urodynamic analysis showed significant increase of leak point pressure and closing pressure. Immunohistochemistry revealed the regeneration of circular muscle mass with normal appearance. Molecular analysis observed the expression of a larger number of target markers. In the immunogenicity analysis, the progenitor group had a scant CD8 lymphocyte. In tumorigenicity, the progenitors showed no teratoma formation. These results suggest that hAFSCs can effectively be differentiated into muscle progenitors in CM and that the hAFSC-derived muscle progenitors are an accessible cell source for the regeneration of injured urethral sphincter. SN - 1598-6357 UR - https://www.unboundmedicine.com/medline/citation/23166409/Human_amniotic_fluid_stem_cell_derived_muscle_progenitor_cell_therapy_for_stress_urinary_incontinence_ L2 - https://jkms.org/DOIx.php?id=10.3346/jkms.2012.27.11.1300 DB - PRIME DP - Unbound Medicine ER -