Tags

Type your tag names separated by a space and hit enter

Filamentous fungi from Plantago lanceolata L. leaves: contribution to the pattern and stability of bioactive metabolites.
Phytochemistry. 2013 Feb; 86:127-36.P

Abstract

The aim of this study was to test contribution of plant-associated microorganism (PAMs) to metabolite stability/instability in a medicinal plant matrix. Therefore, PAM strains were isolated and identified based on relevant DNA sequences from Plantago lanceolata leaves. Sterile water extracts of P. lanceolata were incubated with the isolated strains and antioxidants (ascorbic acid (AA), and EDTA) for 15 days, and changes in the concentrations of chief bioactive constituents (aucubin, catalpol, acteoside (=verbascoside)) were quantified by capillary electrophoresis. Phenolic breakdown-products were identified by GC-MS. PAMs were identified from the genera Epicoccum, Bipolaris, Cladosporium, Leptosphaerulina, Aspergillus, Eurotium and Penicillium (pathongens, endophytes, and other species). Some fungi caused significant decomposition of the chief constituents (p<0.001). Surprisingly, some strains inhibited breakdown of acteoside (p<0.001). Meanwhile, concentration of several phenolic acids increased in fungi-infested extracts (p<0.001). Gentisic acid, 4-hydroxyphenyl acetic acid, 4-hydroxybenzoic acid and hydroxytyrosol were only present when the extract was infested with a PAM. The products are powerful antioxidants and chelators. Concentrations of phenolic acids influenced acteoside stability significantly (p<0.01), as shown by basic data-mining techniques. AA and EDTA also significantly inhibited acteoside breakdown in sterile model solutions (p<0.05). Our results suggest that the phenolic acid mixture (produced during the fungal proliferation) protected acteoside from breakdown, possibly via its antioxidant activity and metal complexing ability. It was shown that PAMs can increase or decrease the stability of chief metabolites in herbal matrices, and can significantly alter the chemical pattern of the plant matrix.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Gonda S
University of Debrecen, Department of Botany, Division of Pharmacognosy, H-4010 Debrecen, Hungary. gondasandor@gmail.com
Kiss A
No affiliation info available
Emri T
No affiliation info available
Batta G
No affiliation info available
Vasas G
No affiliation info available

MeSH

GlucosidesIridoid GlucosidesMolecular StructurePhenolsPlantago

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23168247

Citation

Gonda, Sándor, et al. "Filamentous Fungi From Plantago Lanceolata L. Leaves: Contribution to the Pattern and Stability of Bioactive Metabolites." Phytochemistry, vol. 86, 2013, pp. 127-36.
Gonda S, Kiss A, Emri T, et al. Filamentous fungi from Plantago lanceolata L. leaves: contribution to the pattern and stability of bioactive metabolites. Phytochemistry. 2013;86:127-36.
Gonda, S., Kiss, A., Emri, T., Batta, G., & Vasas, G. (2013). Filamentous fungi from Plantago lanceolata L. leaves: contribution to the pattern and stability of bioactive metabolites. Phytochemistry, 86, 127-36. https://doi.org/10.1016/j.phytochem.2012.10.017
Gonda S, et al. Filamentous Fungi From Plantago Lanceolata L. Leaves: Contribution to the Pattern and Stability of Bioactive Metabolites. Phytochemistry. 2013;86:127-36. PubMed PMID: 23168247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Filamentous fungi from Plantago lanceolata L. leaves: contribution to the pattern and stability of bioactive metabolites. AU - Gonda,Sándor, AU - Kiss,Attila, AU - Emri,Tamás, AU - Batta,Gyula, AU - Vasas,Gábor, Y1 - 2012/11/17/ PY - 2012/09/25/received PY - 2012/10/18/revised PY - 2012/10/22/accepted PY - 2012/11/22/entrez PY - 2012/11/22/pubmed PY - 2013/6/1/medline SP - 127 EP - 36 JF - Phytochemistry JO - Phytochemistry VL - 86 N2 - The aim of this study was to test contribution of plant-associated microorganism (PAMs) to metabolite stability/instability in a medicinal plant matrix. Therefore, PAM strains were isolated and identified based on relevant DNA sequences from Plantago lanceolata leaves. Sterile water extracts of P. lanceolata were incubated with the isolated strains and antioxidants (ascorbic acid (AA), and EDTA) for 15 days, and changes in the concentrations of chief bioactive constituents (aucubin, catalpol, acteoside (=verbascoside)) were quantified by capillary electrophoresis. Phenolic breakdown-products were identified by GC-MS. PAMs were identified from the genera Epicoccum, Bipolaris, Cladosporium, Leptosphaerulina, Aspergillus, Eurotium and Penicillium (pathongens, endophytes, and other species). Some fungi caused significant decomposition of the chief constituents (p<0.001). Surprisingly, some strains inhibited breakdown of acteoside (p<0.001). Meanwhile, concentration of several phenolic acids increased in fungi-infested extracts (p<0.001). Gentisic acid, 4-hydroxyphenyl acetic acid, 4-hydroxybenzoic acid and hydroxytyrosol were only present when the extract was infested with a PAM. The products are powerful antioxidants and chelators. Concentrations of phenolic acids influenced acteoside stability significantly (p<0.01), as shown by basic data-mining techniques. AA and EDTA also significantly inhibited acteoside breakdown in sterile model solutions (p<0.05). Our results suggest that the phenolic acid mixture (produced during the fungal proliferation) protected acteoside from breakdown, possibly via its antioxidant activity and metal complexing ability. It was shown that PAMs can increase or decrease the stability of chief metabolites in herbal matrices, and can significantly alter the chemical pattern of the plant matrix. SN - 1873-3700 UR - https://www.unboundmedicine.com/medline/citation/23168247/Filamentous_fungi_from_Plantago_lanceolata_L__leaves:_contribution_to_the_pattern_and_stability_of_bioactive_metabolites_ DB - PRIME DP - Unbound Medicine ER -