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Follistatin is induced by IL-1β and TNF-α in stromal cells from endometrioma.
Reprod Sci. 2013 Jun; 20(6):675-9.RS

Abstract

The aim of this study is to examine the regulation of follistatin, an activin-binding protein, in endometriosis. Endometrioma stromal cells (EoSCs) were obtained from 9 patients undergoing laparoscopy of the ovarian endometrioma. In cultured EoSCs, interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α), which could induce activin-A, also induced follistatin messenger RNA (mRNA) and protein. The cystic fluid of endometrioma from 8 patients was obtained to measure the concentration of activin-A and follistatin by enzyme-linked immunosorbent assay (ELISA). Also, activin activity in the fluid was examined by erythroid differentiation assay using mouse erythroleukemia F5-5.fl cells. In the cystic fluid of endometrioma, the mean values of activin-A and follistatin concentration were 36.8 ng/mL and 4.0 ng/mL, respectively. In a bioassay, all 8 samples exhibited activin activity, which was equivalent to recombinant activin-A activity of 12.8 ± 1.4 ng/mL. Although follistatin was present in the cystic fluid of endometrioma, the activity of activin, which is an exacerbation factor of endometriosis, was predominant in vivo.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23171678

Citation

Akiyama, Ikumi, et al. "Follistatin Is Induced By IL-1β and TNF-α in Stromal Cells From Endometrioma." Reproductive Sciences (Thousand Oaks, Calif.), vol. 20, no. 6, 2013, pp. 675-9.
Akiyama I, Yoshino O, Osuga Y, et al. Follistatin is induced by IL-1β and TNF-α in stromal cells from endometrioma. Reprod Sci. 2013;20(6):675-9.
Akiyama, I., Yoshino, O., Osuga, Y., Izumi, G., Urata, Y., Hirota, Y., Hirata, T., Harada, M., Koga, K., Ogawa, K., & Kozuma, S. (2013). Follistatin is induced by IL-1β and TNF-α in stromal cells from endometrioma. Reproductive Sciences (Thousand Oaks, Calif.), 20(6), 675-9. https://doi.org/10.1177/1933719112463253
Akiyama I, et al. Follistatin Is Induced By IL-1β and TNF-α in Stromal Cells From Endometrioma. Reprod Sci. 2013;20(6):675-9. PubMed PMID: 23171678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Follistatin is induced by IL-1β and TNF-α in stromal cells from endometrioma. AU - Akiyama,Ikumi, AU - Yoshino,Osamu, AU - Osuga,Yutaka, AU - Izumi,Gentaro, AU - Urata,Yoko, AU - Hirota,Yasushi, AU - Hirata,Tetsuya, AU - Harada,Miyuki, AU - Koga,Kaori, AU - Ogawa,Kenji, AU - Kozuma,Shiro, Y1 - 2012/11/19/ PY - 2012/11/23/entrez PY - 2012/11/23/pubmed PY - 2013/12/16/medline KW - activin KW - endometriosis KW - follistatin KW - inflammatory cytokine SP - 675 EP - 9 JF - Reproductive sciences (Thousand Oaks, Calif.) JO - Reprod Sci VL - 20 IS - 6 N2 - The aim of this study is to examine the regulation of follistatin, an activin-binding protein, in endometriosis. Endometrioma stromal cells (EoSCs) were obtained from 9 patients undergoing laparoscopy of the ovarian endometrioma. In cultured EoSCs, interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α), which could induce activin-A, also induced follistatin messenger RNA (mRNA) and protein. The cystic fluid of endometrioma from 8 patients was obtained to measure the concentration of activin-A and follistatin by enzyme-linked immunosorbent assay (ELISA). Also, activin activity in the fluid was examined by erythroid differentiation assay using mouse erythroleukemia F5-5.fl cells. In the cystic fluid of endometrioma, the mean values of activin-A and follistatin concentration were 36.8 ng/mL and 4.0 ng/mL, respectively. In a bioassay, all 8 samples exhibited activin activity, which was equivalent to recombinant activin-A activity of 12.8 ± 1.4 ng/mL. Although follistatin was present in the cystic fluid of endometrioma, the activity of activin, which is an exacerbation factor of endometriosis, was predominant in vivo. SN - 1933-7205 UR - https://www.unboundmedicine.com/medline/citation/23171678/Follistatin_is_induced_by_IL_1β_and_TNF_α_in_stromal_cells_from_endometrioma_ L2 - http://journals.sagepub.com/doi/full/10.1177/1933719112463253?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -