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Susceptibility of the endocochlear potential to pH and osmolarity changes in the perilymph of the cochlea in the guinea pig.
Eur Arch Otorhinolaryngol. 1990; 247(2):97-9.EA

Abstract

The effects of artificial perilymph at various pH levels and osmolarities on the endocochlear potential (EP) were investigated in a guinea pig model. In 47 ears, the mean EP was 74.5 +/- 0.5 mV in the second turn of the cochlea before perilymphatic perfusion. The artificial perilymph with a pH ranging from 5 to 9 and tonicity less than 1.25 (osmolarity, 345 mosmol/l) did not alter the EP when testings were recorded for at least 1 h. However, after the start of a perfusion with a pH less than 4, the EP declined gradually but recovered to the pre-perfusion level rapidly when the perfusion was stopped. The artificial perilymph with a pH over 10 decreased the EP considerably but showed no recovery, even after cessation of the perfusion. Tonicity more than 1.35 (osmolarity, 372.6 mosmol/l) also decreased the EP, with this decrease paralleling further increases in the tonicity used. From these data, we have concluded that a perilymph perfusate with a pH from 5 to 9 and an osmolarity less than 350 mosmol/l can be used in animal testing without any noxious effects on the EP.

Authors+Show Affiliations

Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2317365

Citation

Wakizono, S, et al. "Susceptibility of the Endocochlear Potential to pH and Osmolarity Changes in the Perilymph of the Cochlea in the Guinea Pig." European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated With the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, vol. 247, no. 2, 1990, pp. 97-9.
Wakizono S, Komune S, Uemura T. Susceptibility of the endocochlear potential to pH and osmolarity changes in the perilymph of the cochlea in the guinea pig. Eur Arch Otorhinolaryngol. 1990;247(2):97-9.
Wakizono, S., Komune, S., & Uemura, T. (1990). Susceptibility of the endocochlear potential to pH and osmolarity changes in the perilymph of the cochlea in the guinea pig. European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated With the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 247(2), 97-9.
Wakizono S, Komune S, Uemura T. Susceptibility of the Endocochlear Potential to pH and Osmolarity Changes in the Perilymph of the Cochlea in the Guinea Pig. Eur Arch Otorhinolaryngol. 1990;247(2):97-9. PubMed PMID: 2317365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Susceptibility of the endocochlear potential to pH and osmolarity changes in the perilymph of the cochlea in the guinea pig. AU - Wakizono,S, AU - Komune,S, AU - Uemura,T, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 97 EP - 9 JF - European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery JO - Eur Arch Otorhinolaryngol VL - 247 IS - 2 N2 - The effects of artificial perilymph at various pH levels and osmolarities on the endocochlear potential (EP) were investigated in a guinea pig model. In 47 ears, the mean EP was 74.5 +/- 0.5 mV in the second turn of the cochlea before perilymphatic perfusion. The artificial perilymph with a pH ranging from 5 to 9 and tonicity less than 1.25 (osmolarity, 345 mosmol/l) did not alter the EP when testings were recorded for at least 1 h. However, after the start of a perfusion with a pH less than 4, the EP declined gradually but recovered to the pre-perfusion level rapidly when the perfusion was stopped. The artificial perilymph with a pH over 10 decreased the EP considerably but showed no recovery, even after cessation of the perfusion. Tonicity more than 1.35 (osmolarity, 372.6 mosmol/l) also decreased the EP, with this decrease paralleling further increases in the tonicity used. From these data, we have concluded that a perilymph perfusate with a pH from 5 to 9 and an osmolarity less than 350 mosmol/l can be used in animal testing without any noxious effects on the EP. SN - 0937-4477 UR - https://www.unboundmedicine.com/medline/citation/2317365/Susceptibility_of_the_endocochlear_potential_to_pH_and_osmolarity_changes_in_the_perilymph_of_the_cochlea_in_the_guinea_pig_ DB - PRIME DP - Unbound Medicine ER -