Tags

Type your tag names separated by a space and hit enter

Isolation and phylogenetic analysis of orf virus from the sheep herd outbreak in northeast China.
BMC Vet Res 2012; 8:229BV

Abstract

BACKGROUND

Orf is a zoonotic and epitheliotrophic contagious disease that mainly affects sheep, goats, wild ruminants, and humans with a worldwide distribution. To date, there is little information on the characterization of ORFV strains that are endemic in Mainland China. In addition, the relationship between the severity of disease and the molecular profile of ORFV strains has not been fully elucidated.

RESULTS

From the recent outbreak of a sheep herd in Nongan, northeast of China, the novel orf virus (ORFV) strain NA1/11 was successfully isolated. Western blot analysis indicated that the NA1/11 strain cross reacts with monoclonal antibody A3 and infected sheep ORFV antiserum. The purified virions revealed the typical ovoid shape when observed by atomic force microscopy. To determine the genetic characteristics of the NA1/11 strain, the sequences of ORFV011 (B2L), ORFV059 (F1L), ORFV109, ORFV110 and ORFv132 (VEGF) genes were amplified and compared with reference parapoxvirus strains. Non-metric multidimensional scaling (nMDS) was performed to analyze the nucleotide similarities between different ORFV strains.

CONCLUSIONS

Phylogenetic analysis based on ORFV 011 nucleotide sequences showed that the NA1/11strain was closely related to Xinjiang and Gansu strains. ORFV110 and ORFV132 genes are highly variable. The results revealed that precise phylogenetic analysis might provide evidence for genetic variation and movement of circulating ORFV strains in Northeast China. In addition, nMDS analysis showed that geographic isolation and animal host are likely major factors resulting in genetic differences between ORFV strains.

Authors+Show Affiliations

Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, N, Guangzhou Avenue 1838, Guangzhou 510515, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23174032

Citation

Li, Wei, et al. "Isolation and Phylogenetic Analysis of Orf Virus From the Sheep Herd Outbreak in Northeast China." BMC Veterinary Research, vol. 8, 2012, p. 229.
Li W, Ning Z, Hao W, et al. Isolation and phylogenetic analysis of orf virus from the sheep herd outbreak in northeast China. BMC Vet Res. 2012;8:229.
Li, W., Ning, Z., Hao, W., Song, D., Gao, F., Zhao, K., ... Luo, S. (2012). Isolation and phylogenetic analysis of orf virus from the sheep herd outbreak in northeast China. BMC Veterinary Research, 8, p. 229. doi:10.1186/1746-6148-8-229.
Li W, et al. Isolation and Phylogenetic Analysis of Orf Virus From the Sheep Herd Outbreak in Northeast China. BMC Vet Res. 2012 Nov 23;8:229. PubMed PMID: 23174032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and phylogenetic analysis of orf virus from the sheep herd outbreak in northeast China. AU - Li,Wei, AU - Ning,Zhangyong, AU - Hao,Wenbo, AU - Song,Deguang, AU - Gao,Feng, AU - Zhao,Kui, AU - Liao,Xiaoqing, AU - Li,Ming, AU - Rock,Daniel L, AU - Luo,Shuhong, Y1 - 2012/11/23/ PY - 2012/05/21/received PY - 2012/10/16/accepted PY - 2012/11/24/entrez PY - 2012/11/24/pubmed PY - 2013/8/14/medline SP - 229 EP - 229 JF - BMC veterinary research JO - BMC Vet. Res. VL - 8 N2 - BACKGROUND: Orf is a zoonotic and epitheliotrophic contagious disease that mainly affects sheep, goats, wild ruminants, and humans with a worldwide distribution. To date, there is little information on the characterization of ORFV strains that are endemic in Mainland China. In addition, the relationship between the severity of disease and the molecular profile of ORFV strains has not been fully elucidated. RESULTS: From the recent outbreak of a sheep herd in Nongan, northeast of China, the novel orf virus (ORFV) strain NA1/11 was successfully isolated. Western blot analysis indicated that the NA1/11 strain cross reacts with monoclonal antibody A3 and infected sheep ORFV antiserum. The purified virions revealed the typical ovoid shape when observed by atomic force microscopy. To determine the genetic characteristics of the NA1/11 strain, the sequences of ORFV011 (B2L), ORFV059 (F1L), ORFV109, ORFV110 and ORFv132 (VEGF) genes were amplified and compared with reference parapoxvirus strains. Non-metric multidimensional scaling (nMDS) was performed to analyze the nucleotide similarities between different ORFV strains. CONCLUSIONS: Phylogenetic analysis based on ORFV 011 nucleotide sequences showed that the NA1/11strain was closely related to Xinjiang and Gansu strains. ORFV110 and ORFV132 genes are highly variable. The results revealed that precise phylogenetic analysis might provide evidence for genetic variation and movement of circulating ORFV strains in Northeast China. In addition, nMDS analysis showed that geographic isolation and animal host are likely major factors resulting in genetic differences between ORFV strains. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/23174032/Isolation_and_phylogenetic_analysis_of_orf_virus_from_the_sheep_herd_outbreak_in_northeast_China_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-229 DB - PRIME DP - Unbound Medicine ER -