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ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis.
Diagn Microbiol Infect Dis. 2013 Feb; 75(2):135-8.DM

Abstract

Melioidosis is caused by the Gram-negative bacterium Burkholderia pseudomallei. The gold standard for diagnosis is culture, which requires at least 3-4 days to obtain a result, hindering successful treatment of acute disease. An indirect haemagglutination assay (IHA) is often used but lacks sensitivity. Approximately half of patients later confirmed culture positive are not detected by IHA at presentation and a subset of patients persistently continue to be IHA negative. More rapid and reliable serologic testing for melioidosis is essential and will improve diagnosis and patient outcome. We have developed an ELISA and a quantitative immuno-polymerase chain reaction assay capable of detecting melioidosis-specific antibodies and demonstrate their validity with IHA-negative sera from patients with melioidosis. These new sensitive assays are based upon a secreted antigenic fraction from B. pseudomallei and will be ideal for the diagnosis of melioidosis in patients in nonendemic regions returning from endemic tropical areas and for seroepidemiologic surveys.

Authors+Show Affiliations

School of Pharmacy and Molecular Sciences, James Cook University, Douglas, QLD 4811, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23177220

Citation

Cooper, Alanna, et al. "ELISA and Immuno-polymerase Chain Reaction Assays for the Sensitive Detection of Melioidosis." Diagnostic Microbiology and Infectious Disease, vol. 75, no. 2, 2013, pp. 135-8.
Cooper A, Williams NL, Morris JL, et al. ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis. Diagn Microbiol Infect Dis. 2013;75(2):135-8.
Cooper, A., Williams, N. L., Morris, J. L., Norton, R. E., Ketheesan, N., & Schaeffer, P. M. (2013). ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis. Diagnostic Microbiology and Infectious Disease, 75(2), 135-8. https://doi.org/10.1016/j.diagmicrobio.2012.10.011
Cooper A, et al. ELISA and Immuno-polymerase Chain Reaction Assays for the Sensitive Detection of Melioidosis. Diagn Microbiol Infect Dis. 2013;75(2):135-8. PubMed PMID: 23177220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis. AU - Cooper,Alanna, AU - Williams,Natasha L, AU - Morris,Jodie L, AU - Norton,Robert E, AU - Ketheesan,Natkunam, AU - Schaeffer,Patrick M, Y1 - 2012/11/21/ PY - 2012/09/05/received PY - 2012/10/17/revised PY - 2012/10/17/accepted PY - 2012/11/27/entrez PY - 2012/11/28/pubmed PY - 2013/9/27/medline SP - 135 EP - 8 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 75 IS - 2 N2 - Melioidosis is caused by the Gram-negative bacterium Burkholderia pseudomallei. The gold standard for diagnosis is culture, which requires at least 3-4 days to obtain a result, hindering successful treatment of acute disease. An indirect haemagglutination assay (IHA) is often used but lacks sensitivity. Approximately half of patients later confirmed culture positive are not detected by IHA at presentation and a subset of patients persistently continue to be IHA negative. More rapid and reliable serologic testing for melioidosis is essential and will improve diagnosis and patient outcome. We have developed an ELISA and a quantitative immuno-polymerase chain reaction assay capable of detecting melioidosis-specific antibodies and demonstrate their validity with IHA-negative sera from patients with melioidosis. These new sensitive assays are based upon a secreted antigenic fraction from B. pseudomallei and will be ideal for the diagnosis of melioidosis in patients in nonendemic regions returning from endemic tropical areas and for seroepidemiologic surveys. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/23177220/ELISA_and_immuno_polymerase_chain_reaction_assays_for_the_sensitive_detection_of_melioidosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(12)00435-X DB - PRIME DP - Unbound Medicine ER -