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Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus.
Chemosphere. 2013 Feb; 90(6):1960-5.C

Abstract

Microbe-assisted phytoremediation has been considered as a promising measure for the remediation of heavy metal-polluted soils. In this study, a metal-tolerance and plant growth-promoting endophytic bacterium JN6 was firstly isolated from roots of Mn-hyperaccumulator Polygonum pubescens grown in metal-contaminated soil and identified as Rahnella sp. based on 16S rDNA gene sequence analysis. Strain JN6 showed very high Cd, Pb and Zn tolerance and effectively solubilized CdCO(3), PbCO(3) and Zn(3)(PO(4))(2) in culture solution. The isolate produced plant growth-promoting substances such as indole-3-acetic acid, siderophore, 1-aminocyclopropane-1-carboxylic deaminase, and also solubilized inorganic phosphate. Based upon its ability in metal tolerance and solubilization, the isolate JN6 was further studied for its effects on the growth and accumulation of Cd, Pb and Zn in Brassica napus (rape) by pot experiments. Rape plants inoculated with the isolate JN6 had significantly higher dry weights, concentrations and uptake of Cd, Pb and Zn in both above-ground and root tissues than those without inoculation grown in soils amended with Cd (25 mg kg(-1)), Pb (200 mg kg(-1)) or Zn (200 mg kg(-1)). The isolate also showed a high level of colonization in tissue interior of rapes. The present results demonstrated that Rahnella sp. JN6 is a valuable microorganism, which can cost-effectively improve the efficiency of phytoremediation in soils contaminated by Cd, Pb and Zn.

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Authors+Show Affiliations

He H
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
Ye Z
No affiliation info available
Yang D
No affiliation info available
Yan J
No affiliation info available
Xiao L
No affiliation info available
Zhong T
No affiliation info available
Yuan M
No affiliation info available
Cai X
No affiliation info available
Fang Z
No affiliation info available
Jing Y
No affiliation info available

MeSH

Base SequenceBiodegradation, EnvironmentalBrassicaCadmiumLeadMetals, HeavyMolecular Sequence DataPolygonumRahnellaSoilSoil PollutantsZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23177711

Citation

He, Huaidong, et al. "Characterization of Endophytic Rahnella Sp. JN6 From Polygonum Pubescens and Its Potential in Promoting Growth and Cd, Pb, Zn Uptake By Brassica Napus." Chemosphere, vol. 90, no. 6, 2013, pp. 1960-5.
He H, Ye Z, Yang D, et al. Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus. Chemosphere. 2013;90(6):1960-5.
He, H., Ye, Z., Yang, D., Yan, J., Xiao, L., Zhong, T., Yuan, M., Cai, X., Fang, Z., & Jing, Y. (2013). Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus. Chemosphere, 90(6), 1960-5. https://doi.org/10.1016/j.chemosphere.2012.10.057
He H, et al. Characterization of Endophytic Rahnella Sp. JN6 From Polygonum Pubescens and Its Potential in Promoting Growth and Cd, Pb, Zn Uptake By Brassica Napus. Chemosphere. 2013;90(6):1960-5. PubMed PMID: 23177711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus. AU - He,Huaidong, AU - Ye,Zhihong, AU - Yang,Danjing, AU - Yan,Junlan, AU - Xiao,Li, AU - Zhong,Ting, AU - Yuan,Ming, AU - Cai,Xinde, AU - Fang,Zhanqiang, AU - Jing,Yuanxiao, Y1 - 2012/11/21/ PY - 2012/06/19/received PY - 2012/10/13/revised PY - 2012/10/21/accepted PY - 2012/11/27/entrez PY - 2012/11/28/pubmed PY - 2013/5/15/medline SP - 1960 EP - 5 JF - Chemosphere JO - Chemosphere VL - 90 IS - 6 N2 - Microbe-assisted phytoremediation has been considered as a promising measure for the remediation of heavy metal-polluted soils. In this study, a metal-tolerance and plant growth-promoting endophytic bacterium JN6 was firstly isolated from roots of Mn-hyperaccumulator Polygonum pubescens grown in metal-contaminated soil and identified as Rahnella sp. based on 16S rDNA gene sequence analysis. Strain JN6 showed very high Cd, Pb and Zn tolerance and effectively solubilized CdCO(3), PbCO(3) and Zn(3)(PO(4))(2) in culture solution. The isolate produced plant growth-promoting substances such as indole-3-acetic acid, siderophore, 1-aminocyclopropane-1-carboxylic deaminase, and also solubilized inorganic phosphate. Based upon its ability in metal tolerance and solubilization, the isolate JN6 was further studied for its effects on the growth and accumulation of Cd, Pb and Zn in Brassica napus (rape) by pot experiments. Rape plants inoculated with the isolate JN6 had significantly higher dry weights, concentrations and uptake of Cd, Pb and Zn in both above-ground and root tissues than those without inoculation grown in soils amended with Cd (25 mg kg(-1)), Pb (200 mg kg(-1)) or Zn (200 mg kg(-1)). The isolate also showed a high level of colonization in tissue interior of rapes. The present results demonstrated that Rahnella sp. JN6 is a valuable microorganism, which can cost-effectively improve the efficiency of phytoremediation in soils contaminated by Cd, Pb and Zn. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/23177711/Characterization_of_endophytic_Rahnella_sp__JN6_from_Polygonum_pubescens_and_its_potential_in_promoting_growth_and_Cd_Pb_Zn_uptake_by_Brassica_napus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(12)01296-9 DB - PRIME DP - Unbound Medicine ER -