Tags

Type your tag names separated by a space and hit enter

Usefulness of the organ culture system when villous height/crypt depth ratio, intraepithelial lymphocyte count, or serum antibody tests are not diagnostic for celiac disease.
Transl Res 2013; 161(3):172-80TR

Abstract

The existence of mild forms of celiac disease (CD) can make the histology-based diagnosis difficult to reach. Since anti-endomysium (EMA) and anti-tissue transglutaminase (anti-tTG) are detectable in culture supernatants of duodenal biopsies from CD patients, our aim was to assess if this system can support the histology in the diagnostic work-up. A total of 559 suspected CD patients underwent serum EMA/anti-tTG detection, upper endoscopy with duodenal biopsy sampling, histologic analysis, and organ culture to detect EMA/anti-tTG in supernatants. A subgroup of 30 patients with organ culture positive results were put on a gluten-free diet (GFD). Their gluten-dependency was evaluated by the psychological general well-being and beck depression inventory indexes. Statistical analysis was performed by Cohen k inter-test, Friedman test, and Dunn multiple comparison. Two hundred forty-one out of 559 (43.1%) patients showed intestinal villous atrophy, whereas serum and organ culture EMA/anti-tTG were positive in 293/559 (52.4%) and 334/559 (59.7%) patients, respectively. The strength of agreement resulted good for serology vs histology (k = 0.730), good for organ culture vs histology (k = 0.662), and very good for serology vs organ culture (k = 0.852). After 12 months of GFD, psychological general well-being index significantly increased, and beck depression inventory index significantly decreased (P < 0.001 for each one). Data highlight the organ culture system as a useful tool to assist the histology in diagnosing CD, mainly in cases without villous atrophy or in seronegative patients. The marked improvement in quality of life after a GFD further supports the reliability of this system in diagnosing CD.

Authors+Show Affiliations

Department of Internal Medicine and Medical Specialties, Sapienza University, 00161 Rome, Italy. antonio.picarelli@uniroma1.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23177794

Citation

Picarelli, Antonio, et al. "Usefulness of the Organ Culture System when Villous Height/crypt Depth Ratio, Intraepithelial Lymphocyte Count, or Serum Antibody Tests Are Not Diagnostic for Celiac Disease." Translational Research : the Journal of Laboratory and Clinical Medicine, vol. 161, no. 3, 2013, pp. 172-80.
Picarelli A, Di Tola M, Marino M, et al. Usefulness of the organ culture system when villous height/crypt depth ratio, intraepithelial lymphocyte count, or serum antibody tests are not diagnostic for celiac disease. Transl Res. 2013;161(3):172-80.
Picarelli, A., Di Tola, M., Marino, M., Libanori, V., Borghini, R., Salvi, E., ... Corazziari, E. (2013). Usefulness of the organ culture system when villous height/crypt depth ratio, intraepithelial lymphocyte count, or serum antibody tests are not diagnostic for celiac disease. Translational Research : the Journal of Laboratory and Clinical Medicine, 161(3), pp. 172-80. doi:10.1016/j.trsl.2012.10.010.
Picarelli A, et al. Usefulness of the Organ Culture System when Villous Height/crypt Depth Ratio, Intraepithelial Lymphocyte Count, or Serum Antibody Tests Are Not Diagnostic for Celiac Disease. Transl Res. 2013;161(3):172-80. PubMed PMID: 23177794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usefulness of the organ culture system when villous height/crypt depth ratio, intraepithelial lymphocyte count, or serum antibody tests are not diagnostic for celiac disease. AU - Picarelli,Antonio, AU - Di Tola,Marco, AU - Marino,Mariacatia, AU - Libanori,Valerio, AU - Borghini,Raffaele, AU - Salvi,Elisa, AU - Donato,Giuseppe, AU - Vitolo,Domenico, AU - Tiberti,Antonio, AU - Marcheggiano,Adriana, AU - Bassotti,Gabrio, AU - Corazziari,Enrico, Y1 - 2012/11/20/ PY - 2012/09/04/received PY - 2012/10/24/revised PY - 2012/10/25/accepted PY - 2012/11/27/entrez PY - 2012/11/28/pubmed PY - 2013/4/23/medline SP - 172 EP - 80 JF - Translational research : the journal of laboratory and clinical medicine JO - Transl Res VL - 161 IS - 3 N2 - The existence of mild forms of celiac disease (CD) can make the histology-based diagnosis difficult to reach. Since anti-endomysium (EMA) and anti-tissue transglutaminase (anti-tTG) are detectable in culture supernatants of duodenal biopsies from CD patients, our aim was to assess if this system can support the histology in the diagnostic work-up. A total of 559 suspected CD patients underwent serum EMA/anti-tTG detection, upper endoscopy with duodenal biopsy sampling, histologic analysis, and organ culture to detect EMA/anti-tTG in supernatants. A subgroup of 30 patients with organ culture positive results were put on a gluten-free diet (GFD). Their gluten-dependency was evaluated by the psychological general well-being and beck depression inventory indexes. Statistical analysis was performed by Cohen k inter-test, Friedman test, and Dunn multiple comparison. Two hundred forty-one out of 559 (43.1%) patients showed intestinal villous atrophy, whereas serum and organ culture EMA/anti-tTG were positive in 293/559 (52.4%) and 334/559 (59.7%) patients, respectively. The strength of agreement resulted good for serology vs histology (k = 0.730), good for organ culture vs histology (k = 0.662), and very good for serology vs organ culture (k = 0.852). After 12 months of GFD, psychological general well-being index significantly increased, and beck depression inventory index significantly decreased (P < 0.001 for each one). Data highlight the organ culture system as a useful tool to assist the histology in diagnosing CD, mainly in cases without villous atrophy or in seronegative patients. The marked improvement in quality of life after a GFD further supports the reliability of this system in diagnosing CD. SN - 1878-1810 UR - https://www.unboundmedicine.com/medline/citation/23177794/Usefulness_of_the_organ_culture_system_when_villous_height/crypt_depth_ratio_intraepithelial_lymphocyte_count_or_serum_antibody_tests_are_not_diagnostic_for_celiac_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1931-5244(12)00371-4 DB - PRIME DP - Unbound Medicine ER -