TY - JOUR T1 - Physiological and enzymatic comparison between Pichia stipitis and recombinant Saccharomyces cerevisiae on xylose fermentation. AU - Guo,Changying, AU - Jiang,Ning, Y1 - 2012/11/20/ PY - 2012/03/06/received PY - 2012/11/03/accepted PY - 2012/11/28/entrez PY - 2012/11/28/pubmed PY - 2013/7/19/medline SP - 541 EP - 7 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 29 IS - 3 N2 - In order to better understand the differences in xylose metabolism between natural xylose-utilizing Pichia stipitis and metabolically engineered Saccharomyces cerevisiae, we constructed a series of recombinant S. cerevisiae strains with different xylose reductase/xylitol dehydrogenase/xylulokinase activity ratios by integrating xylitol dehydrogenase gene (XYL2) into the chromosome with variable copies and heterogeneously expressing xylose reductase gene (XYL1) and endogenous xylulokinase gene (XKS1). The strain with the highest specific xylose uptake rate and ethanol productivity on pure xylose fermentation was selected to compare to P. stipitis under oxygen-limited condition. Physiological and enzymatic comparison showed that they have different patterns of xylose metabolism and NADPH generation. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/23180545/Physiological_and_enzymatic_comparison_between_Pichia_stipitis_and_recombinant_Saccharomyces_cerevisiae_on_xylose_fermentation_ L2 - https://dx.doi.org/10.1007/s11274-012-1208-x DB - PRIME DP - Unbound Medicine ER -