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The discovery of vitamin B(12).
Ann Nutr Metab 2012; 61(3):239-45AN

Abstract

The discovery of vitamin B(12), the elucidation of its role in metabolism, and the effects and treatment of its deficiency occurred in distinct phases over more than 100 years, and it was the subject of two separate Nobel Prizes. The valuable contribution of clinical reports and studies of patients with pernicious anemia throughout the 19th century resulted in enough clinical definition to allow Minot and Murphy to put together the first hallmark study on treatment of the condition, leading them to a Nobel Prize. These researchers were not the first to suggest that an inadequacy of nutrients was the cause of pernicious anemia, but their particular input was a carefully designed intervention in well-characterized pernicious anemia patients, of a special diet containing large amounts of liver. They found consistent improvement in the clinical and blood status of all subjects, most of whom remained on remission indefinitely. After the successful intervention studies, the next advance was made by Castle who discovered that a gastric component, which he called intrinsic factor, was missing in pernicious anemia. Many years later, intrinsic factor was found to be a glycoprotein that formed a complex with vitamin B(12), promoting its absorption through ileal receptors. The vitamin was isolated by two groups simultaneously and was crystallized and characterized in the laboratory of Dorothy Hodgkin, contributing to her Nobel Prize in 1964. Subsequently, the various biochemical roles of vitamin B(12) were elucidated, including its important interaction with folate and their common link with megaloblastic anemia. Many of the early clinical studies recognized that vitamin B(12) deficiency also caused a severe neuropathy leading to paralysis and death, while post mortem analysis demonstrated spinal cord demyelination. Vitamin B(12) is still the subject of intense research and, in particular, its role in preventing these irreversible neurological lesions remains unclear.

Authors+Show Affiliations

School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland. jscott @ tcd.ieNo affiliation info available

Pub Type(s)

Historical Article
Journal Article
Review

Language

eng

PubMed ID

23183296

Citation

Scott, John M., and Anne M. Molloy. "The Discovery of Vitamin B(12)." Annals of Nutrition & Metabolism, vol. 61, no. 3, 2012, pp. 239-45.
Scott JM, Molloy AM. The discovery of vitamin B(12). Ann Nutr Metab. 2012;61(3):239-45.
Scott, J. M., & Molloy, A. M. (2012). The discovery of vitamin B(12). Annals of Nutrition & Metabolism, 61(3), pp. 239-45. doi:10.1159/000343114.
Scott JM, Molloy AM. The Discovery of Vitamin B(12). Ann Nutr Metab. 2012;61(3):239-45. PubMed PMID: 23183296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The discovery of vitamin B(12). AU - Scott,John M, AU - Molloy,Anne M, Y1 - 2012/11/26/ PY - 2012/11/28/entrez PY - 2012/11/28/pubmed PY - 2013/4/30/medline SP - 239 EP - 45 JF - Annals of nutrition & metabolism JO - Ann. Nutr. Metab. VL - 61 IS - 3 N2 - The discovery of vitamin B(12), the elucidation of its role in metabolism, and the effects and treatment of its deficiency occurred in distinct phases over more than 100 years, and it was the subject of two separate Nobel Prizes. The valuable contribution of clinical reports and studies of patients with pernicious anemia throughout the 19th century resulted in enough clinical definition to allow Minot and Murphy to put together the first hallmark study on treatment of the condition, leading them to a Nobel Prize. These researchers were not the first to suggest that an inadequacy of nutrients was the cause of pernicious anemia, but their particular input was a carefully designed intervention in well-characterized pernicious anemia patients, of a special diet containing large amounts of liver. They found consistent improvement in the clinical and blood status of all subjects, most of whom remained on remission indefinitely. After the successful intervention studies, the next advance was made by Castle who discovered that a gastric component, which he called intrinsic factor, was missing in pernicious anemia. Many years later, intrinsic factor was found to be a glycoprotein that formed a complex with vitamin B(12), promoting its absorption through ileal receptors. The vitamin was isolated by two groups simultaneously and was crystallized and characterized in the laboratory of Dorothy Hodgkin, contributing to her Nobel Prize in 1964. Subsequently, the various biochemical roles of vitamin B(12) were elucidated, including its important interaction with folate and their common link with megaloblastic anemia. Many of the early clinical studies recognized that vitamin B(12) deficiency also caused a severe neuropathy leading to paralysis and death, while post mortem analysis demonstrated spinal cord demyelination. Vitamin B(12) is still the subject of intense research and, in particular, its role in preventing these irreversible neurological lesions remains unclear. SN - 1421-9697 UR - https://www.unboundmedicine.com/medline/citation/23183296/The_discovery_of_vitamin_B(12) L2 - https://www.karger.com?DOI=10.1159/000343114 DB - PRIME DP - Unbound Medicine ER -