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Flow sorting and molecular cytogenetic identification of individual chromosomes of Dasypyrum villosum L. (H. villosa) by a single DNA probe.
PLoS One. 2012; 7(11):e50151.Plos

Abstract

Dasypyrum villosum (L.) Candargy (sin. Haynaldia villosa) is an annual wild diploid grass species (2n = 2x = 14; genome VV) belonging to the Poaceae family, which is considered to be an important source of biotic and abiotic stress resistance genes for wheat breeding. Enhanced characterization of D. villosum chromosomes can facilitate exploitation of its gene pool and its use in wheat breeding programs. Here we present the cytogenetic identification of D. villosum chromosomes on slide by fluorescent in situ hybridization (FISH), with the GAA simple sequence repeat (SSR) as a probe. We also describe the isolation and the flow cytometric analysis of D. villosum chromosomes in suspension, resulting in a distinguished flow karyotype. Chromosomes were flow sorted into three fractions, according their DNA content, one of which was composed of a single type of chromosome, namely 6 V, sorted with over 85% purity. Chromosome 6 V is known to carry genes to code for important resistance and seed storage characteristics, and its isolation represents a new source of genetic traits and specific markers useful for wheat improvement.

Authors+Show Affiliations

ENEA, CASACCIA Research Center, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23185561

Citation

Grosso, Valentina, et al. "Flow Sorting and Molecular Cytogenetic Identification of Individual Chromosomes of Dasypyrum Villosum L. (H. Villosa) By a Single DNA Probe." PloS One, vol. 7, no. 11, 2012, pp. e50151.
Grosso V, Farina A, Gennaro A, et al. Flow sorting and molecular cytogenetic identification of individual chromosomes of Dasypyrum villosum L. (H. villosa) by a single DNA probe. PLoS One. 2012;7(11):e50151.
Grosso, V., Farina, A., Gennaro, A., Giorgi, D., & Lucretti, S. (2012). Flow sorting and molecular cytogenetic identification of individual chromosomes of Dasypyrum villosum L. (H. villosa) by a single DNA probe. PloS One, 7(11), e50151. https://doi.org/10.1371/journal.pone.0050151
Grosso V, et al. Flow Sorting and Molecular Cytogenetic Identification of Individual Chromosomes of Dasypyrum Villosum L. (H. Villosa) By a Single DNA Probe. PLoS One. 2012;7(11):e50151. PubMed PMID: 23185561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow sorting and molecular cytogenetic identification of individual chromosomes of Dasypyrum villosum L. (H. villosa) by a single DNA probe. AU - Grosso,Valentina, AU - Farina,Anna, AU - Gennaro,Andrea, AU - Giorgi,Debora, AU - Lucretti,Sergio, Y1 - 2012/11/20/ PY - 2012/05/07/received PY - 2012/10/19/accepted PY - 2012/11/28/entrez PY - 2012/11/28/pubmed PY - 2013/5/15/medline SP - e50151 EP - e50151 JF - PloS one JO - PLoS One VL - 7 IS - 11 N2 - Dasypyrum villosum (L.) Candargy (sin. Haynaldia villosa) is an annual wild diploid grass species (2n = 2x = 14; genome VV) belonging to the Poaceae family, which is considered to be an important source of biotic and abiotic stress resistance genes for wheat breeding. Enhanced characterization of D. villosum chromosomes can facilitate exploitation of its gene pool and its use in wheat breeding programs. Here we present the cytogenetic identification of D. villosum chromosomes on slide by fluorescent in situ hybridization (FISH), with the GAA simple sequence repeat (SSR) as a probe. We also describe the isolation and the flow cytometric analysis of D. villosum chromosomes in suspension, resulting in a distinguished flow karyotype. Chromosomes were flow sorted into three fractions, according their DNA content, one of which was composed of a single type of chromosome, namely 6 V, sorted with over 85% purity. Chromosome 6 V is known to carry genes to code for important resistance and seed storage characteristics, and its isolation represents a new source of genetic traits and specific markers useful for wheat improvement. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23185561/Flow_sorting_and_molecular_cytogenetic_identification_of_individual_chromosomes_of_Dasypyrum_villosum_L___H__villosa__by_a_single_DNA_probe_ L2 - https://dx.plos.org/10.1371/journal.pone.0050151 DB - PRIME DP - Unbound Medicine ER -