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Epirubicin loaded with propylene glycol liposomes significantly overcomes multidrug resistance in breast cancer.
Cancer Lett. 2013 Mar 01; 330(1):74-83.CL

Abstract

Multidrug resistance (MDR) is one of the major reasons for the failure of cancer chemotherapy. A newly reported liposome carrier, propylene glycol liposomes (EPI-PG-liposomes) were made to load epirubicin (EPI) which enhanced EPI absorption in MDR tumor cells to overcome the drug resistance. MDA-MB 435 and their mutant resistant (MDA-MB 435/ADR) cells were used to examine the cellular uptake and P-gp function in vitro for EPI-PG-liposomes by fluorescence microscopy and FCM, respectively. Mammary tumor model was also established to investigate the tumor growth inhibition and pharmacodynamics of EPI-PG-liposomes in vivo. Morphology evaluation showed that EPI-PG-liposomes had a homogeneous spherical shape with an average diameter of 182 nm. Based on cell viability assay, fluorescent microscopy examination, and EPI uptake assay, EPI-PG-liposomes exhibited an effective growth inhibition not only in MDA-MB-435 cells, but also in MDA-MB 435/ADR cells. EPI-PG-liposomes have high permeability not only on tumor cell membrane, but also on cell nucleus membrane. P-gp function assay showed that the anticancer action of EPI-PG-liposomes was not related to P-gp efflux pump, suggesting that PG-liposomes would not affect the normal physiological functions of membrane proteins. EPI-PG-liposomes also showed a better antitumor efficacy compared to EPI solution alone. With high entrapment efficiency, spherical morphology and effective inhibition on MDR cancer cells, EPI-PG-liposomes may represent a better chemotherapeutic vectors for cancer targeted therapy.

Authors+Show Affiliations

Wenzhou Medical College, Wenzhou City, Zhejiang Province 325035, China. zhaoyz@mzmc.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23186833

Citation

Zhao, Ying-Zheng, et al. "Epirubicin Loaded With Propylene Glycol Liposomes Significantly Overcomes Multidrug Resistance in Breast Cancer." Cancer Letters, vol. 330, no. 1, 2013, pp. 74-83.
Zhao YZ, Dai DD, Lu CT, et al. Epirubicin loaded with propylene glycol liposomes significantly overcomes multidrug resistance in breast cancer. Cancer Lett. 2013;330(1):74-83.
Zhao, Y. Z., Dai, D. D., Lu, C. T., Chen, L. J., Lin, M., Shen, X. T., Li, X. K., Zhang, M., Jiang, X., Jin, R. R., Li, X., Lv, H. F., Cai, L., & Huang, P. T. (2013). Epirubicin loaded with propylene glycol liposomes significantly overcomes multidrug resistance in breast cancer. Cancer Letters, 330(1), 74-83. https://doi.org/10.1016/j.canlet.2012.11.031
Zhao YZ, et al. Epirubicin Loaded With Propylene Glycol Liposomes Significantly Overcomes Multidrug Resistance in Breast Cancer. Cancer Lett. 2013 Mar 1;330(1):74-83. PubMed PMID: 23186833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epirubicin loaded with propylene glycol liposomes significantly overcomes multidrug resistance in breast cancer. AU - Zhao,Ying-Zheng, AU - Dai,Dan-Dan, AU - Lu,Cui-Tao, AU - Chen,Li-Juan, AU - Lin,Min, AU - Shen,Xiao-Tong, AU - Li,Xiao-Kun, AU - Zhang,Ming, AU - Jiang,Xi, AU - Jin,Rong-Rong, AU - Li,Xing, AU - Lv,Hai-Feng, AU - Cai,Lu, AU - Huang,Pin-Tong, Y1 - 2012/11/24/ PY - 2012/10/31/received PY - 2012/11/15/revised PY - 2012/11/16/accepted PY - 2012/11/29/entrez PY - 2012/11/29/pubmed PY - 2013/5/10/medline SP - 74 EP - 83 JF - Cancer letters JO - Cancer Lett VL - 330 IS - 1 N2 - Multidrug resistance (MDR) is one of the major reasons for the failure of cancer chemotherapy. A newly reported liposome carrier, propylene glycol liposomes (EPI-PG-liposomes) were made to load epirubicin (EPI) which enhanced EPI absorption in MDR tumor cells to overcome the drug resistance. MDA-MB 435 and their mutant resistant (MDA-MB 435/ADR) cells were used to examine the cellular uptake and P-gp function in vitro for EPI-PG-liposomes by fluorescence microscopy and FCM, respectively. Mammary tumor model was also established to investigate the tumor growth inhibition and pharmacodynamics of EPI-PG-liposomes in vivo. Morphology evaluation showed that EPI-PG-liposomes had a homogeneous spherical shape with an average diameter of 182 nm. Based on cell viability assay, fluorescent microscopy examination, and EPI uptake assay, EPI-PG-liposomes exhibited an effective growth inhibition not only in MDA-MB-435 cells, but also in MDA-MB 435/ADR cells. EPI-PG-liposomes have high permeability not only on tumor cell membrane, but also on cell nucleus membrane. P-gp function assay showed that the anticancer action of EPI-PG-liposomes was not related to P-gp efflux pump, suggesting that PG-liposomes would not affect the normal physiological functions of membrane proteins. EPI-PG-liposomes also showed a better antitumor efficacy compared to EPI solution alone. With high entrapment efficiency, spherical morphology and effective inhibition on MDR cancer cells, EPI-PG-liposomes may represent a better chemotherapeutic vectors for cancer targeted therapy. SN - 1872-7980 UR - https://www.unboundmedicine.com/medline/citation/23186833/Epirubicin_loaded_with_propylene_glycol_liposomes_significantly_overcomes_multidrug_resistance_in_breast_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3835(12)00689-1 DB - PRIME DP - Unbound Medicine ER -