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Enrichment of two isoflavone aglycones in black soymilk by immobilized β-glucosidase on solid carriers.
J Agric Food Chem. 2012 Dec 26; 60(51):12540-6.JA

Abstract

A catalytic system for deglycosylation of isoflavone in black soybean milk was established. β-Glucosidase which was covalently immobilized onto the glass microspheres exhibited a significant efficiency for the conversion of pNPG to p-nitrophenol over other carriers. The optimum temperature for pNPG hydrolysis was 40 °C, and complete reaction can be reached in 30 min. Operational reusability was confirmed for more than 40 batch reactions. Moreover, the storage stability verification demonstrated that the glass microsphere catalytic system was capable of sustaining its highest catalytic activity for 40 days. The kinetic parameters, including rate constant (K) at which isoflavone glycosides deglycosylation were determined, the time (τ(50)) in which 50% of isoflavone glycosides deglycosylation was reached, and the time (τ(complete)) required to achieve complete isoflavone glycosides deglycosylation, were 0.35 ± 0.04 min(-1), 2.04 ± 0.25 min, and 30 min (for daidzin) and 0.65 ± 0.03 min(-1), 1.19 ± 0.08 min, and 20 min (for genistin), respectively. HPLC results revealed that this enzyme system took only 30 min to reach complete isoflavone deglycosylation and the aglycone content in the total isoflavones in black soymilk was enriched by 51.42 ± 0.17% under a 30 min treatment by the glass microsphere enzymatic system.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chen KI
Graduate Institute of Food Science & Technology, National Taiwan University , No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
Lo YC
No affiliation info available
Su NW
No affiliation info available
Chou CC
No affiliation info available
Cheng KC
No affiliation info available

MeSH

Enzyme StabilityEnzymes, ImmobilizedFood, FortifiedGlassGlycosidesGlycosylationHydrolysisIsoflavonesKineticsMicrospheresNitrophenolsSoy Milkbeta-Glucosidase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23190054

Citation

Chen, Kuan-I, et al. "Enrichment of Two Isoflavone Aglycones in Black Soymilk By Immobilized Β-glucosidase On Solid Carriers." Journal of Agricultural and Food Chemistry, vol. 60, no. 51, 2012, pp. 12540-6.
Chen KI, Lo YC, Su NW, et al. Enrichment of two isoflavone aglycones in black soymilk by immobilized β-glucosidase on solid carriers. J Agric Food Chem. 2012;60(51):12540-6.
Chen, K. I., Lo, Y. C., Su, N. W., Chou, C. C., & Cheng, K. C. (2012). Enrichment of two isoflavone aglycones in black soymilk by immobilized β-glucosidase on solid carriers. Journal of Agricultural and Food Chemistry, 60(51), 12540-6. https://doi.org/10.1021/jf304405t
Chen KI, et al. Enrichment of Two Isoflavone Aglycones in Black Soymilk By Immobilized Β-glucosidase On Solid Carriers. J Agric Food Chem. 2012 Dec 26;60(51):12540-6. PubMed PMID: 23190054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enrichment of two isoflavone aglycones in black soymilk by immobilized β-glucosidase on solid carriers. AU - Chen,Kuan-I, AU - Lo,Yi-Chen, AU - Su,Nan-Wei, AU - Chou,Cheng-Chun, AU - Cheng,Kuan-Chen, Y1 - 2012/12/13/ PY - 2012/11/30/entrez PY - 2012/11/30/pubmed PY - 2013/3/13/medline SP - 12540 EP - 6 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 51 N2 - A catalytic system for deglycosylation of isoflavone in black soybean milk was established. β-Glucosidase which was covalently immobilized onto the glass microspheres exhibited a significant efficiency for the conversion of pNPG to p-nitrophenol over other carriers. The optimum temperature for pNPG hydrolysis was 40 °C, and complete reaction can be reached in 30 min. Operational reusability was confirmed for more than 40 batch reactions. Moreover, the storage stability verification demonstrated that the glass microsphere catalytic system was capable of sustaining its highest catalytic activity for 40 days. The kinetic parameters, including rate constant (K) at which isoflavone glycosides deglycosylation were determined, the time (τ(50)) in which 50% of isoflavone glycosides deglycosylation was reached, and the time (τ(complete)) required to achieve complete isoflavone glycosides deglycosylation, were 0.35 ± 0.04 min(-1), 2.04 ± 0.25 min, and 30 min (for daidzin) and 0.65 ± 0.03 min(-1), 1.19 ± 0.08 min, and 20 min (for genistin), respectively. HPLC results revealed that this enzyme system took only 30 min to reach complete isoflavone deglycosylation and the aglycone content in the total isoflavones in black soymilk was enriched by 51.42 ± 0.17% under a 30 min treatment by the glass microsphere enzymatic system. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/23190054/Enrichment_of_two_isoflavone_aglycones_in_black_soymilk_by_immobilized_β_glucosidase_on_solid_carriers_ L2 - https://doi.org/10.1021/jf304405t DB - PRIME DP - Unbound Medicine ER -