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Detection of the plasmid-encoded fosfomycin resistance gene fosA3 in Escherichia coli of food-animal origin.
J Antimicrob Chemother. 2013 Apr; 68(4):766-70.JA

Abstract

OBJECTIVES

To investigate the occurrence of plasmid-mediated fosfomycin resistance genes among Escherichia coli from food animals in China.

METHODS

A total of 892 E. coli isolates collected from individual pigs (n=368), chickens (n=196), ducks (n=261), geese (n=35), pigeons (n=20) and partridges (n=12) in Guangdong Province during 2002-08 were screened for the presence of fosA3, fosA and fosC2 by PCR amplification and sequencing. The clonal relationship of fosA3-positive isolates, plasmid content and other associated resistance genes were also characterized.

RESULTS

Twelve (1.3%) E. coli isolates showed resistance to fosfomycin and 10 (1.1%) isolates (4 from pigs, 2 from chickens, 2 from ducks, 1 from a goose and 1 from a pigeon) were positive for fosA3. None of the E. coli isolates was positive for fosA or fosC2. All of the isolates carrying fosA3 were CTX-M producers, and three of them carried rmtB. Most of the fosA3-harbouring isolates were found to be clonally unrelated. The fosA3 genes were flanked by IS26. Two fosA3 genes co-localized with rmtB and blaCTX-M-65 on indistinguishable F33:A-:B- plasmids that carried three addiction systems (pemI/pemK, hok/mok/sok and srnB). Four, one and one fosA3 genes were found to be associated with IncN (ST8 type), IncI1 and F2:A-:B- plasmids, respectively.

CONCLUSIONS

We discovered that fosA3 is always associated with blaCTX-M, which facilitates its quick dispersal. The emergence of fosA3 in food animals could impact on human medicine by the potential transfer of resistance through the food chain.

Authors+Show Affiliations

College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University (SCAU), Guangzhou, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23190765

Citation

Hou, Jianxia, et al. "Detection of the Plasmid-encoded Fosfomycin Resistance Gene fosA3 in Escherichia Coli of Food-animal Origin." The Journal of Antimicrobial Chemotherapy, vol. 68, no. 4, 2013, pp. 766-70.
Hou J, Yang X, Zeng Z, et al. Detection of the plasmid-encoded fosfomycin resistance gene fosA3 in Escherichia coli of food-animal origin. J Antimicrob Chemother. 2013;68(4):766-70.
Hou, J., Yang, X., Zeng, Z., Lv, L., Yang, T., Lin, D., & Liu, J. H. (2013). Detection of the plasmid-encoded fosfomycin resistance gene fosA3 in Escherichia coli of food-animal origin. The Journal of Antimicrobial Chemotherapy, 68(4), 766-70. https://doi.org/10.1093/jac/dks465
Hou J, et al. Detection of the Plasmid-encoded Fosfomycin Resistance Gene fosA3 in Escherichia Coli of Food-animal Origin. J Antimicrob Chemother. 2013;68(4):766-70. PubMed PMID: 23190765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of the plasmid-encoded fosfomycin resistance gene fosA3 in Escherichia coli of food-animal origin. AU - Hou,Jianxia, AU - Yang,Xiaoyun, AU - Zeng,Zhenling, AU - Lv,Luchao, AU - Yang,Tong, AU - Lin,Dachuan, AU - Liu,Jian-Hua, Y1 - 2012/11/28/ PY - 2012/11/30/entrez PY - 2012/11/30/pubmed PY - 2013/8/27/medline SP - 766 EP - 70 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 68 IS - 4 N2 - OBJECTIVES: To investigate the occurrence of plasmid-mediated fosfomycin resistance genes among Escherichia coli from food animals in China. METHODS: A total of 892 E. coli isolates collected from individual pigs (n=368), chickens (n=196), ducks (n=261), geese (n=35), pigeons (n=20) and partridges (n=12) in Guangdong Province during 2002-08 were screened for the presence of fosA3, fosA and fosC2 by PCR amplification and sequencing. The clonal relationship of fosA3-positive isolates, plasmid content and other associated resistance genes were also characterized. RESULTS: Twelve (1.3%) E. coli isolates showed resistance to fosfomycin and 10 (1.1%) isolates (4 from pigs, 2 from chickens, 2 from ducks, 1 from a goose and 1 from a pigeon) were positive for fosA3. None of the E. coli isolates was positive for fosA or fosC2. All of the isolates carrying fosA3 were CTX-M producers, and three of them carried rmtB. Most of the fosA3-harbouring isolates were found to be clonally unrelated. The fosA3 genes were flanked by IS26. Two fosA3 genes co-localized with rmtB and blaCTX-M-65 on indistinguishable F33:A-:B- plasmids that carried three addiction systems (pemI/pemK, hok/mok/sok and srnB). Four, one and one fosA3 genes were found to be associated with IncN (ST8 type), IncI1 and F2:A-:B- plasmids, respectively. CONCLUSIONS: We discovered that fosA3 is always associated with blaCTX-M, which facilitates its quick dispersal. The emergence of fosA3 in food animals could impact on human medicine by the potential transfer of resistance through the food chain. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/23190765/Detection_of_the_plasmid_encoded_fosfomycin_resistance_gene_fosA3_in_Escherichia_coli_of_food_animal_origin_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dks465 DB - PRIME DP - Unbound Medicine ER -