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Simultaneous determination of 4-substituted cathinones (4-MMC, 4-MEC and 4-FMC) in human urine by HPLC-DAD.
J Chromatogr Sci. 2013 Oct; 51(9):861-6.JC

Abstract

This study developed a selective and rapid high-performance liquid chromatography-diode array detection method for the confirmation of different cathinone derivates in human urine. Samples were prepared by solid-phase extraction (SPE) using procaine hydrochloride as the internal standard. The chromatographic separation was performed on a Kinetex PFP column using isocratic elution. The mobile phase was composed of a mixture of acetonitrile (33%, v/v) and 0.005M ammonium trifluoroacetate buffer (67%, v/v; pH 4.93 ± 0.03) with a flow rate of 0.350 mL/min. The diode array detection was performed at 262 nm. The method was linear over the concentration range of 25-2,400 ng/mL. Intra-day and inter-day precision values for cathinones were less than 1.26% (relative standard deviation). The limit of detection for any compounds extracted from human urine by the optimized SPE method was 40 ng/mL and the limit of quantification was 100 ng/mL in the urine. The recovery rate of SPE was between 71 and 82% with a lower relative standard deviation than 2.35%.

Authors+Show Affiliations

1Institute of Forensic Medicine, University of Pécs, H-7624 Pécs, Szigeti str. 12., Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23192736

Citation

Mayer, Mátyás, et al. "Simultaneous Determination of 4-substituted Cathinones (4-MMC, 4-MEC and 4-FMC) in Human Urine By HPLC-DAD." Journal of Chromatographic Science, vol. 51, no. 9, 2013, pp. 861-6.
Mayer M, Benko A, Huszár A, et al. Simultaneous determination of 4-substituted cathinones (4-MMC, 4-MEC and 4-FMC) in human urine by HPLC-DAD. J Chromatogr Sci. 2013;51(9):861-6.
Mayer, M., Benko, A., Huszár, A., Sipos, K., Lajtai, A., Lakatos, A., & Porpáczy, Z. (2013). Simultaneous determination of 4-substituted cathinones (4-MMC, 4-MEC and 4-FMC) in human urine by HPLC-DAD. Journal of Chromatographic Science, 51(9), 861-6. https://doi.org/10.1093/chromsci/bms183
Mayer M, et al. Simultaneous Determination of 4-substituted Cathinones (4-MMC, 4-MEC and 4-FMC) in Human Urine By HPLC-DAD. J Chromatogr Sci. 2013;51(9):861-6. PubMed PMID: 23192736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of 4-substituted cathinones (4-MMC, 4-MEC and 4-FMC) in human urine by HPLC-DAD. AU - Mayer,Mátyás, AU - Benko,András, AU - Huszár,András, AU - Sipos,Katalin, AU - Lajtai,Anikó, AU - Lakatos,Agnes, AU - Porpáczy,Zoltán, Y1 - 2012/11/28/ PY - 2012/11/30/entrez PY - 2012/11/30/pubmed PY - 2014/4/15/medline SP - 861 EP - 6 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 51 IS - 9 N2 - This study developed a selective and rapid high-performance liquid chromatography-diode array detection method for the confirmation of different cathinone derivates in human urine. Samples were prepared by solid-phase extraction (SPE) using procaine hydrochloride as the internal standard. The chromatographic separation was performed on a Kinetex PFP column using isocratic elution. The mobile phase was composed of a mixture of acetonitrile (33%, v/v) and 0.005M ammonium trifluoroacetate buffer (67%, v/v; pH 4.93 ± 0.03) with a flow rate of 0.350 mL/min. The diode array detection was performed at 262 nm. The method was linear over the concentration range of 25-2,400 ng/mL. Intra-day and inter-day precision values for cathinones were less than 1.26% (relative standard deviation). The limit of detection for any compounds extracted from human urine by the optimized SPE method was 40 ng/mL and the limit of quantification was 100 ng/mL in the urine. The recovery rate of SPE was between 71 and 82% with a lower relative standard deviation than 2.35%. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/23192736/Simultaneous_determination_of_4_substituted_cathinones__4_MMC_4_MEC_and_4_FMC__in_human_urine_by_HPLC_DAD_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bms183 DB - PRIME DP - Unbound Medicine ER -