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Neurotrophin serum concentrations and polymorphisms of neurotrophins and their receptors in children with asthma.
Respir Med. 2013 Jan; 107(1):30-6.RM

Abstract

BACKGROUND

In the recent years numerous studies have analysed the effects of neurotrophins on allergic inflammation in airway diseases reporting increased neurotrophin levels locally in the airways as well as in serum of asthmatic patients. We aimed to investigate if levels of neurotrophins in serum of asthmatic children are influenced by the genotype of functional variants within genes encoding analysed neurotrophins and their specific receptors.

METHODS

In the study we included 98 children diagnosed with asthma. Genotyping of 9 polymorphisms located in neurotrophins genes and their receptors genes was done with use of TaqMan SNP genotyping assays or PCR-RFLP. The serum levels of four neurotrophins (BDNF, NGF, NTF3, NTF4) were analysed during exacerbation of asthma symptoms with use of DuoSet ELISA Development Kit (R&D).

RESULTS

The two patients with the genetic variant A/A of NTRK1 (rs6334) showed significantly higher NGF serum concentrations (113.4 and 218.1 pg/mL) as compared to the mean NGF serum concentrations in the total group of patients (34.8 pg/mL). We also observed a significant epistatic interactions between variants of NGF rs6330 and NTRK1 rs6334 that influenced NGF serum level (P = 0.0004). Analysis of four neurotrophins serum levels in relation to different genotypes of analysed neurotrophins genes showed no significant differences among analysed asthmatic children.

CONCLUSIONS

Our results suggest that, among analysed neurotrophins, NGF serum levels may be influenced by the genotype of NTRK1 gene individually as well as in the interaction with NGF functional genetic variant suggesting their involvement in allergic inflammation in asthma. Serum levels of the other neurotrophins do not seem to be affected by the variants in the analysed genes.

Authors+Show Affiliations

Laboratory of Molecular and Cell Biology, Department of Pediatric Pulmonology, Allergy and Clinical Immunology, IIIrd Department of Pediatrics, Poznan University of Medical Sciences, Poznan, Poland. alszczep@amp.edu.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23195334

Citation

Szczepankiewicz, Aleksandra, et al. "Neurotrophin Serum Concentrations and Polymorphisms of Neurotrophins and Their Receptors in Children With Asthma." Respiratory Medicine, vol. 107, no. 1, 2013, pp. 30-6.
Szczepankiewicz A, Rachel M, Sobkowiak P, et al. Neurotrophin serum concentrations and polymorphisms of neurotrophins and their receptors in children with asthma. Respir Med. 2013;107(1):30-6.
Szczepankiewicz, A., Rachel, M., Sobkowiak, P., Kycler, Z., Wojsyk-Banaszak, I., Schöneich, N., Szczawińska-Popłonyk, A., & Bręborowicz, A. (2013). Neurotrophin serum concentrations and polymorphisms of neurotrophins and their receptors in children with asthma. Respiratory Medicine, 107(1), 30-6. https://doi.org/10.1016/j.rmed.2012.09.024
Szczepankiewicz A, et al. Neurotrophin Serum Concentrations and Polymorphisms of Neurotrophins and Their Receptors in Children With Asthma. Respir Med. 2013;107(1):30-6. PubMed PMID: 23195334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurotrophin serum concentrations and polymorphisms of neurotrophins and their receptors in children with asthma. AU - Szczepankiewicz,Aleksandra, AU - Rachel,Marta, AU - Sobkowiak,Paulina, AU - Kycler,Zdzisława, AU - Wojsyk-Banaszak,Irena, AU - Schöneich,Natalia, AU - Szczawińska-Popłonyk,Aleksandra, AU - Bręborowicz,Anna, Y1 - 2012/11/26/ PY - 2012/02/09/received PY - 2012/09/23/revised PY - 2012/09/26/accepted PY - 2012/12/1/entrez PY - 2012/12/1/pubmed PY - 2013/6/5/medline SP - 30 EP - 6 JF - Respiratory medicine JO - Respir Med VL - 107 IS - 1 N2 - BACKGROUND: In the recent years numerous studies have analysed the effects of neurotrophins on allergic inflammation in airway diseases reporting increased neurotrophin levels locally in the airways as well as in serum of asthmatic patients. We aimed to investigate if levels of neurotrophins in serum of asthmatic children are influenced by the genotype of functional variants within genes encoding analysed neurotrophins and their specific receptors. METHODS: In the study we included 98 children diagnosed with asthma. Genotyping of 9 polymorphisms located in neurotrophins genes and their receptors genes was done with use of TaqMan SNP genotyping assays or PCR-RFLP. The serum levels of four neurotrophins (BDNF, NGF, NTF3, NTF4) were analysed during exacerbation of asthma symptoms with use of DuoSet ELISA Development Kit (R&D). RESULTS: The two patients with the genetic variant A/A of NTRK1 (rs6334) showed significantly higher NGF serum concentrations (113.4 and 218.1 pg/mL) as compared to the mean NGF serum concentrations in the total group of patients (34.8 pg/mL). We also observed a significant epistatic interactions between variants of NGF rs6330 and NTRK1 rs6334 that influenced NGF serum level (P = 0.0004). Analysis of four neurotrophins serum levels in relation to different genotypes of analysed neurotrophins genes showed no significant differences among analysed asthmatic children. CONCLUSIONS: Our results suggest that, among analysed neurotrophins, NGF serum levels may be influenced by the genotype of NTRK1 gene individually as well as in the interaction with NGF functional genetic variant suggesting their involvement in allergic inflammation in asthma. Serum levels of the other neurotrophins do not seem to be affected by the variants in the analysed genes. SN - 1532-3064 UR - https://www.unboundmedicine.com/medline/citation/23195334/Neurotrophin_serum_concentrations_and_polymorphisms_of_neurotrophins_and_their_receptors_in_children_with_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(12)00406-4 DB - PRIME DP - Unbound Medicine ER -