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In situ enzymatic ascorbic acid production as electron donor for CdS quantum dots equipped TiO2 nanotubes: a general and efficient approach for new photoelectrochemical immunoassay.
Anal Chem. 2012 Dec 18; 84(24):10518-21.AC

Abstract

In this work, a novel photoelectrochemical (PEC) immunoanalysis format was developed for sensitive and specific detection of prostate-specific antigen (PSA) based on an in situ electron donor producing approach. Thioglycolic acid-capped CdS quantum dots (QDs) equipped TiO(2) nanotubes (NTs) were fabricated via a facile electrostatic adsorption method. The coupling of CdS QDs and TiO(2) NTs results in an enhanced excitation and photo-to-electric conversion efficiency. Using alkaline phosphatase catalytic chemistry to in situ generate ascorbic acid for electron donating, an exquisite immunosandwich protocol was successfully constructed for the PSA assay due to the dependence of the photocurrent signal on the concentration of electron donor. This work opens a different perspective for transducer design in PEC detection and provides a general format for future development of PEC immunoanalysis.

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23198754

Citation

Zhao, Wei-Wei, et al. "In Situ Enzymatic Ascorbic Acid Production as Electron Donor for CdS Quantum Dots Equipped TiO2 Nanotubes: a General and Efficient Approach for New Photoelectrochemical Immunoassay." Analytical Chemistry, vol. 84, no. 24, 2012, pp. 10518-21.
Zhao WW, Ma ZY, Yan DY, et al. In situ enzymatic ascorbic acid production as electron donor for CdS quantum dots equipped TiO2 nanotubes: a general and efficient approach for new photoelectrochemical immunoassay. Anal Chem. 2012;84(24):10518-21.
Zhao, W. W., Ma, Z. Y., Yan, D. Y., Xu, J. J., & Chen, H. Y. (2012). In situ enzymatic ascorbic acid production as electron donor for CdS quantum dots equipped TiO2 nanotubes: a general and efficient approach for new photoelectrochemical immunoassay. Analytical Chemistry, 84(24), 10518-21. https://doi.org/10.1021/ac3028799
Zhao WW, et al. In Situ Enzymatic Ascorbic Acid Production as Electron Donor for CdS Quantum Dots Equipped TiO2 Nanotubes: a General and Efficient Approach for New Photoelectrochemical Immunoassay. Anal Chem. 2012 Dec 18;84(24):10518-21. PubMed PMID: 23198754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ enzymatic ascorbic acid production as electron donor for CdS quantum dots equipped TiO2 nanotubes: a general and efficient approach for new photoelectrochemical immunoassay. AU - Zhao,Wei-Wei, AU - Ma,Zheng-Yuan, AU - Yan,Dong-Yang, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2012/12/04/ PY - 2012/12/4/entrez PY - 2012/12/4/pubmed PY - 2014/4/23/medline SP - 10518 EP - 21 JF - Analytical chemistry JO - Anal Chem VL - 84 IS - 24 N2 - In this work, a novel photoelectrochemical (PEC) immunoanalysis format was developed for sensitive and specific detection of prostate-specific antigen (PSA) based on an in situ electron donor producing approach. Thioglycolic acid-capped CdS quantum dots (QDs) equipped TiO(2) nanotubes (NTs) were fabricated via a facile electrostatic adsorption method. The coupling of CdS QDs and TiO(2) NTs results in an enhanced excitation and photo-to-electric conversion efficiency. Using alkaline phosphatase catalytic chemistry to in situ generate ascorbic acid for electron donating, an exquisite immunosandwich protocol was successfully constructed for the PSA assay due to the dependence of the photocurrent signal on the concentration of electron donor. This work opens a different perspective for transducer design in PEC detection and provides a general format for future development of PEC immunoanalysis. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/23198754/In_situ_enzymatic_ascorbic_acid_production_as_electron_donor_for_CdS_quantum_dots_equipped_TiO2_nanotubes:_a_general_and_efficient_approach_for_new_photoelectrochemical_immunoassay_ L2 - https://doi.org/10.1021/ac3028799 DB - PRIME DP - Unbound Medicine ER -