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Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats.
BMC Complement Altern Med. 2012 Dec 02; 12:241.BC

Abstract

BACKGROUND

Evidence is building steadily on the effectiveness of acupuncture therapy in pain relief and repeated acupuncture-induced pain relief is accompanied by improvement of hippocampal neural synaptic plasticity. To further test the cellular and molecular changes underlying analgesic effect of acupuncture, the global change of acupuncture associated protein profiles in the hippocampus under neuropathic pain condition was profiled.

METHODS

The chronic constrictive injury (CCI) model was established by ligature of the unilateral sciatic nerve in adult Wistar rats. Rats were randomized into normal control (NC) group, CCI group, and CCI with electroacupuncture (EA) stimulation group. EA was applied to bilateral Zusanli (ST36) and Yanglingquan (GB34) in the EA group. Differentially expressed proteins in the hippocampus in the three groups were identified by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The functional clustering of the identified proteins was analyzed by Mascot software.

RESULTS

After CCI, the thermal pain threshold of the affected hind footpad was decreased and was reversed gradually by 12 sessions of acupuncture treatment. Following EA, there were 19 hippocampal proteins identified with significant changes in expression (>2-fold), which are involved in metabolic, physiological, and cellular processes. The top three canonical pathways identified were "cysteine metabolism", "valine, leucine, and isoleucine degradation" and "mitogen-activated protein kinase (MAPK) signaling".

CONCLUSIONS

These data suggest that the analgesic effect of EA is mediated by regulation of hippocampal proteins related to amino acid metabolism and activation of the MAPK signaling pathway.

Authors+Show Affiliations

Department of Physiology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23198761

Citation

Gao, Yong-Hui, et al. "Differential Proteomics Analysis of the Analgesic Effect of Electroacupuncture Intervention in the Hippocampus Following Neuropathic Pain in Rats." BMC Complementary and Alternative Medicine, vol. 12, 2012, p. 241.
Gao YH, Chen SP, Wang JY, et al. Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats. BMC Complement Altern Med. 2012;12:241.
Gao, Y. H., Chen, S. P., Wang, J. Y., Qiao, L. N., Meng, F. Y., Xu, Q. L., & Liu, J. L. (2012). Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats. BMC Complementary and Alternative Medicine, 12, 241. https://doi.org/10.1186/1472-6882-12-241
Gao YH, et al. Differential Proteomics Analysis of the Analgesic Effect of Electroacupuncture Intervention in the Hippocampus Following Neuropathic Pain in Rats. BMC Complement Altern Med. 2012 Dec 2;12:241. PubMed PMID: 23198761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats. AU - Gao,Yong-Hui, AU - Chen,Shu-Ping, AU - Wang,Jun-Ying, AU - Qiao,Li-Na, AU - Meng,Fan-Ying, AU - Xu,Qiu-Ling, AU - Liu,Jun-Ling, Y1 - 2012/12/02/ PY - 2012/09/20/received PY - 2012/11/27/accepted PY - 2012/12/4/entrez PY - 2012/12/4/pubmed PY - 2013/5/29/medline SP - 241 EP - 241 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 12 N2 - BACKGROUND: Evidence is building steadily on the effectiveness of acupuncture therapy in pain relief and repeated acupuncture-induced pain relief is accompanied by improvement of hippocampal neural synaptic plasticity. To further test the cellular and molecular changes underlying analgesic effect of acupuncture, the global change of acupuncture associated protein profiles in the hippocampus under neuropathic pain condition was profiled. METHODS: The chronic constrictive injury (CCI) model was established by ligature of the unilateral sciatic nerve in adult Wistar rats. Rats were randomized into normal control (NC) group, CCI group, and CCI with electroacupuncture (EA) stimulation group. EA was applied to bilateral Zusanli (ST36) and Yanglingquan (GB34) in the EA group. Differentially expressed proteins in the hippocampus in the three groups were identified by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The functional clustering of the identified proteins was analyzed by Mascot software. RESULTS: After CCI, the thermal pain threshold of the affected hind footpad was decreased and was reversed gradually by 12 sessions of acupuncture treatment. Following EA, there were 19 hippocampal proteins identified with significant changes in expression (>2-fold), which are involved in metabolic, physiological, and cellular processes. The top three canonical pathways identified were "cysteine metabolism", "valine, leucine, and isoleucine degradation" and "mitogen-activated protein kinase (MAPK) signaling". CONCLUSIONS: These data suggest that the analgesic effect of EA is mediated by regulation of hippocampal proteins related to amino acid metabolism and activation of the MAPK signaling pathway. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/23198761/Differential_proteomics_analysis_of_the_analgesic_effect_of_electroacupuncture_intervention_in_the_hippocampus_following_neuropathic_pain_in_rats_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-12-241 DB - PRIME DP - Unbound Medicine ER -