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OsRab11 and OsGAP1 are essential for the vesicle trafficking of the vacuolar H(+)-ATPase OsVHA-a1 under high salinity conditions.
Plant Sci. 2013 Jan; 198:58-71.PS

Abstract

To understand the molecular mechanism of the plant vacuolar H(+)-ATPase in endocytic trafficking and adaptation to high salinity, yeast two-hybrid assay, IP-western hybridization, trafficking assay, RT- and qRT-PCR analyses and growth assay were performed here. To confirm the interaction between OsVHA-a1 and OsGAP1, pull-down assay and Co-IP were performed in vitro and in vivo, respectively. qRT-PCR analysis revealed that the transcription of OsVHA-a1, OsGAP1 and OsRab11 was induced under high salinity. Through the protoplast-based trafficking assay, OsVHA-a1 localized predominantly from the TGN to the PVC under stressed conditions. In addition, both OsGAP1 (R385A) and OsRab11 (S28N) mutants did not interact with OsVHA-a1, and blocked the vesicular trafficking of OsVHA-a1 to the PVC. In a seedling growth assay using the dominant negative OsRab11 (S28N), this mutant was much more sensitive to high salinity than the wild-type. Furthermore, the trafficking assay using isolated vacuoles demonstrated directly that OsGAP1 targeted to the tonoplast of the central vacuole under high salinity. Taken together, it is suggested that OsGAP1 and OsRab11 are essential for the vesicle trafficking of OsVHA-a1 to the PVC and/or the central vacuole under high salinity.

Authors+Show Affiliations

Division of Applied Life Sciences (BK21), Graduate School of Gyeongsang National University, Jinju 660-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23199687

Citation

Son, Young Sim, et al. "OsRab11 and OsGAP1 Are Essential for the Vesicle Trafficking of the Vacuolar H(+)-ATPase OsVHA-a1 Under High Salinity Conditions." Plant Science : an International Journal of Experimental Plant Biology, vol. 198, 2013, pp. 58-71.
Son YS, Im CH, Kim DW, et al. OsRab11 and OsGAP1 are essential for the vesicle trafficking of the vacuolar H(+)-ATPase OsVHA-a1 under high salinity conditions. Plant Sci. 2013;198:58-71.
Son, Y. S., Im, C. H., Kim, D. W., & Bahk, J. D. (2013). OsRab11 and OsGAP1 are essential for the vesicle trafficking of the vacuolar H(+)-ATPase OsVHA-a1 under high salinity conditions. Plant Science : an International Journal of Experimental Plant Biology, 198, 58-71. https://doi.org/10.1016/j.plantsci.2012.09.003
Son YS, et al. OsRab11 and OsGAP1 Are Essential for the Vesicle Trafficking of the Vacuolar H(+)-ATPase OsVHA-a1 Under High Salinity Conditions. Plant Sci. 2013;198:58-71. PubMed PMID: 23199687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - OsRab11 and OsGAP1 are essential for the vesicle trafficking of the vacuolar H(+)-ATPase OsVHA-a1 under high salinity conditions. AU - Son,Young Sim, AU - Im,Chak Han, AU - Kim,Dae Won, AU - Bahk,Jeong Dong, Y1 - 2012/09/18/ PY - 2012/07/10/received PY - 2012/09/03/revised PY - 2012/09/12/accepted PY - 2012/12/4/entrez PY - 2012/12/4/pubmed PY - 2013/5/25/medline SP - 58 EP - 71 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 198 N2 - To understand the molecular mechanism of the plant vacuolar H(+)-ATPase in endocytic trafficking and adaptation to high salinity, yeast two-hybrid assay, IP-western hybridization, trafficking assay, RT- and qRT-PCR analyses and growth assay were performed here. To confirm the interaction between OsVHA-a1 and OsGAP1, pull-down assay and Co-IP were performed in vitro and in vivo, respectively. qRT-PCR analysis revealed that the transcription of OsVHA-a1, OsGAP1 and OsRab11 was induced under high salinity. Through the protoplast-based trafficking assay, OsVHA-a1 localized predominantly from the TGN to the PVC under stressed conditions. In addition, both OsGAP1 (R385A) and OsRab11 (S28N) mutants did not interact with OsVHA-a1, and blocked the vesicular trafficking of OsVHA-a1 to the PVC. In a seedling growth assay using the dominant negative OsRab11 (S28N), this mutant was much more sensitive to high salinity than the wild-type. Furthermore, the trafficking assay using isolated vacuoles demonstrated directly that OsGAP1 targeted to the tonoplast of the central vacuole under high salinity. Taken together, it is suggested that OsGAP1 and OsRab11 are essential for the vesicle trafficking of OsVHA-a1 to the PVC and/or the central vacuole under high salinity. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/23199687/OsRab11_and_OsGAP1_are_essential_for_the_vesicle_trafficking_of_the_vacuolar_H_+__ATPase_OsVHA_a1_under_high_salinity_conditions_ DB - PRIME DP - Unbound Medicine ER -