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The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice.
Plant Cell. 2012 Nov; 24(11):4748-62.PC

Abstract

Although the functions of a few effector proteins produced by bacterial and oomycete plant pathogens have been elucidated in recent years, information for the vast majority of pathogen effectors is still lacking, particularly for those of plant-pathogenic fungi. Here, we show that the avirulence effector AvrPiz-t from the rice blast fungus Magnaporthe oryzae preferentially accumulates in the specialized structure called the biotrophic interfacial complex and is then translocated into rice (Oryza sativa) cells. Ectopic expression of AvrPiz-t in transgenic rice suppresses the flg22- and chitin-induced generation of reactive oxygen species (ROS) and enhances susceptibility to M. oryzae, indicating that AvrPiz-t functions to suppress pathogen-associated molecular pattern (PAMP)-triggered immunity in rice. Interaction assays show that AvrPiz-t suppresses the ubiquitin ligase activity of the rice RING E3 ubiquitin ligase APIP6 and that, in return, APIP6 ubiquitinates AvrPiz-t in vitro. Interestingly, agroinfection assays reveal that AvrPiz-t and AvrPiz-t Interacting Protein 6 (APIP6) are both degraded when coexpressed in Nicotiana benthamiana. Silencing of APIP6 in transgenic rice leads to a significant reduction of flg22-induced ROS generation, suppression of defense-related gene expression, and enhanced susceptibility of rice plants to M. oryzae. Taken together, our results reveal a mechanism in which a fungal effector targets the host ubiquitin proteasome system for the suppression of PAMP-triggered immunity in plants.

Authors+Show Affiliations

Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23204406

Citation

Park, Chan-Ho, et al. "The Magnaporthe Oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-associated Molecular Pattern-triggered Immunity in Rice." The Plant Cell, vol. 24, no. 11, 2012, pp. 4748-62.
Park CH, Chen S, Shirsekar G, et al. The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice. Plant Cell. 2012;24(11):4748-62.
Park, C. H., Chen, S., Shirsekar, G., Zhou, B., Khang, C. H., Songkumarn, P., Afzal, A. J., Ning, Y., Wang, R., Bellizzi, M., Valent, B., & Wang, G. L. (2012). The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice. The Plant Cell, 24(11), 4748-62. https://doi.org/10.1105/tpc.112.105429
Park CH, et al. The Magnaporthe Oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-associated Molecular Pattern-triggered Immunity in Rice. Plant Cell. 2012;24(11):4748-62. PubMed PMID: 23204406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice. AU - Park,Chan-Ho, AU - Chen,Songbiao, AU - Shirsekar,Gautam, AU - Zhou,Bo, AU - Khang,Chang Hyun, AU - Songkumarn,Pattavipha, AU - Afzal,Ahmed J, AU - Ning,Yuese, AU - Wang,Ruyi, AU - Bellizzi,Maria, AU - Valent,Barbara, AU - Wang,Guo-Liang, Y1 - 2012/11/30/ PY - 2012/12/4/entrez PY - 2012/12/4/pubmed PY - 2013/8/22/medline SP - 4748 EP - 62 JF - The Plant cell JO - Plant Cell VL - 24 IS - 11 N2 - Although the functions of a few effector proteins produced by bacterial and oomycete plant pathogens have been elucidated in recent years, information for the vast majority of pathogen effectors is still lacking, particularly for those of plant-pathogenic fungi. Here, we show that the avirulence effector AvrPiz-t from the rice blast fungus Magnaporthe oryzae preferentially accumulates in the specialized structure called the biotrophic interfacial complex and is then translocated into rice (Oryza sativa) cells. Ectopic expression of AvrPiz-t in transgenic rice suppresses the flg22- and chitin-induced generation of reactive oxygen species (ROS) and enhances susceptibility to M. oryzae, indicating that AvrPiz-t functions to suppress pathogen-associated molecular pattern (PAMP)-triggered immunity in rice. Interaction assays show that AvrPiz-t suppresses the ubiquitin ligase activity of the rice RING E3 ubiquitin ligase APIP6 and that, in return, APIP6 ubiquitinates AvrPiz-t in vitro. Interestingly, agroinfection assays reveal that AvrPiz-t and AvrPiz-t Interacting Protein 6 (APIP6) are both degraded when coexpressed in Nicotiana benthamiana. Silencing of APIP6 in transgenic rice leads to a significant reduction of flg22-induced ROS generation, suppression of defense-related gene expression, and enhanced susceptibility of rice plants to M. oryzae. Taken together, our results reveal a mechanism in which a fungal effector targets the host ubiquitin proteasome system for the suppression of PAMP-triggered immunity in plants. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/23204406/The_Magnaporthe_oryzae_effector_AvrPiz_t_targets_the_RING_E3_ubiquitin_ligase_APIP6_to_suppress_pathogen_associated_molecular_pattern_triggered_immunity_in_rice_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=23204406 DB - PRIME DP - Unbound Medicine ER -