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Effects of dietary cholesterol on antioxidant capacity, non-specific immune response, and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets.
Fish Shellfish Immunol. 2013 Jan; 34(1):324-31.FS

Abstract

This study evaluated the effects of dietary cholesterol on antioxidant capacity, non-specific immune response and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets. Fish were fed diets supplemented with graded cholesterol levels (0 [control], 0.3, 0.6, 0.9, 1.2, and 1.5%) for nine weeks. The fish were then challenged by A. hydrophila and their survival rate recorded for the next week. Dietary cholesterol supplementation generally increased the serum and hepatic superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (TAC) activities, but decreased the serum and hepatic malondialdehyde (MDA) contents. Further, the hepatic CAT and serum SOD, CAT, and TAC activities were significantly higher in fish fed diets supplemented with 0.9 or 1.2% cholesterol compared to those fed the control diet, whereas the serum and hepatic MDA contents were significantly lower. The respiratory burst activity, alternative complement activity, and hepatic lysozyme activity increased steadily when the supplemental cholesterol was increased by up to 1.2% and then declined with further addition. The serum lysozyme activity and phagocytic activity increased steadily with increasing dietary supplemental cholesterol level up to 0.9% and then declined with further addition. Dietary cholesterol supplementation generally enhanced the protection against A. hydrophila infection, and fish fed diets supplemented with 0.9 or 1.2% cholesterol exhibited the highest post-challenge survival rate. The results indicated that cholesterol may be under-supplied in rainbow trout fed soybean meal-based diets, and dietary cholesterol supplementation (0.9-1.2%) contributed to improved immune response and disease resistance of rainbow trout against A. hydrophila.

Authors+Show Affiliations

College of Animal Science and Technology, Yunnan Agricultural University, Panlong District, Kunming 650201, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23207478

Citation

Deng, Junming, et al. "Effects of Dietary Cholesterol On Antioxidant Capacity, Non-specific Immune Response, and Resistance to Aeromonas Hydrophila in Rainbow Trout (Oncorhynchus Mykiss) Fed Soybean Meal-based Diets." Fish & Shellfish Immunology, vol. 34, no. 1, 2013, pp. 324-31.
Deng J, Kang B, Tao L, et al. Effects of dietary cholesterol on antioxidant capacity, non-specific immune response, and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets. Fish Shellfish Immunol. 2013;34(1):324-31.
Deng, J., Kang, B., Tao, L., Rong, H., & Zhang, X. (2013). Effects of dietary cholesterol on antioxidant capacity, non-specific immune response, and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets. Fish & Shellfish Immunology, 34(1), 324-31. https://doi.org/10.1016/j.fsi.2012.11.008
Deng J, et al. Effects of Dietary Cholesterol On Antioxidant Capacity, Non-specific Immune Response, and Resistance to Aeromonas Hydrophila in Rainbow Trout (Oncorhynchus Mykiss) Fed Soybean Meal-based Diets. Fish Shellfish Immunol. 2013;34(1):324-31. PubMed PMID: 23207478.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary cholesterol on antioxidant capacity, non-specific immune response, and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets. AU - Deng,Junming, AU - Kang,Bin, AU - Tao,Linli, AU - Rong,Hua, AU - Zhang,Xi, Y1 - 2012/12/01/ PY - 2012/08/04/received PY - 2012/10/18/revised PY - 2012/11/03/accepted PY - 2012/12/5/entrez PY - 2012/12/5/pubmed PY - 2013/6/19/medline SP - 324 EP - 31 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 34 IS - 1 N2 - This study evaluated the effects of dietary cholesterol on antioxidant capacity, non-specific immune response and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets. Fish were fed diets supplemented with graded cholesterol levels (0 [control], 0.3, 0.6, 0.9, 1.2, and 1.5%) for nine weeks. The fish were then challenged by A. hydrophila and their survival rate recorded for the next week. Dietary cholesterol supplementation generally increased the serum and hepatic superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (TAC) activities, but decreased the serum and hepatic malondialdehyde (MDA) contents. Further, the hepatic CAT and serum SOD, CAT, and TAC activities were significantly higher in fish fed diets supplemented with 0.9 or 1.2% cholesterol compared to those fed the control diet, whereas the serum and hepatic MDA contents were significantly lower. The respiratory burst activity, alternative complement activity, and hepatic lysozyme activity increased steadily when the supplemental cholesterol was increased by up to 1.2% and then declined with further addition. The serum lysozyme activity and phagocytic activity increased steadily with increasing dietary supplemental cholesterol level up to 0.9% and then declined with further addition. Dietary cholesterol supplementation generally enhanced the protection against A. hydrophila infection, and fish fed diets supplemented with 0.9 or 1.2% cholesterol exhibited the highest post-challenge survival rate. The results indicated that cholesterol may be under-supplied in rainbow trout fed soybean meal-based diets, and dietary cholesterol supplementation (0.9-1.2%) contributed to improved immune response and disease resistance of rainbow trout against A. hydrophila. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/23207478/Effects_of_dietary_cholesterol_on_antioxidant_capacity_non_specific_immune_response_and_resistance_to_Aeromonas_hydrophila_in_rainbow_trout__Oncorhynchus_mykiss__fed_soybean_meal_based_diets_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(12)00410-X DB - PRIME DP - Unbound Medicine ER -