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A pathogenic role for CD4+ T cells during Chikungunya virus infection in mice.
J Immunol. 2013 Jan 01; 190(1):259-69.JI

Abstract

Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. Injury to the joint is believed to occur because of viral and host immune-mediated effects. However, the exact involvement of the different immune mediators in CHIKV-induced pathogenesis is unknown. In this study, we assessed the roles of T cells in primary CHIKV infection, virus replication and dissemination, and virus persistence, as well as in the mediation of disease severity in adult RAG2(-/-), CD4(-/-), CD8(-/-), and wild-type CHIKV C57BL/6J mice and in wild-type mice depleted of CD4(+) or CD8(+) T cells after Ab treatment. CHIKV-specific T cells in the spleen and footpad were investigated using IFN-γ ELISPOT. Interestingly, our results indicated that CHIKV-specific CD4(+), but not CD8(+), T cells are essential for the development of joint swelling without any effect on virus replication and dissemination. Infection in IFN-γ(-/-) mice demonstrated that pathogenic CD4(+) T cells do not mediate inflammation via an IFN-γ-mediated pathway. Taken together, these observations strongly indicate that mechanisms of joint pathology induced by CHIKV in mice resemble those in humans and differ from infections caused by other arthritogenic viruses, such as Ross River virus.

Authors+Show Affiliations

Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, Singapore 138648.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23209328

Citation

Teo, Teck-Hui, et al. "A Pathogenic Role for CD4+ T Cells During Chikungunya Virus Infection in Mice." Journal of Immunology (Baltimore, Md. : 1950), vol. 190, no. 1, 2013, pp. 259-69.
Teo TH, Lum FM, Claser C, et al. A pathogenic role for CD4+ T cells during Chikungunya virus infection in mice. J Immunol. 2013;190(1):259-69.
Teo, T. H., Lum, F. M., Claser, C., Lulla, V., Lulla, A., Merits, A., Rénia, L., & Ng, L. F. (2013). A pathogenic role for CD4+ T cells during Chikungunya virus infection in mice. Journal of Immunology (Baltimore, Md. : 1950), 190(1), 259-69. https://doi.org/10.4049/jimmunol.1202177
Teo TH, et al. A Pathogenic Role for CD4+ T Cells During Chikungunya Virus Infection in Mice. J Immunol. 2013 Jan 1;190(1):259-69. PubMed PMID: 23209328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A pathogenic role for CD4+ T cells during Chikungunya virus infection in mice. AU - Teo,Teck-Hui, AU - Lum,Fok-Moon, AU - Claser,Carla, AU - Lulla,Valeria, AU - Lulla,Aleksei, AU - Merits,Andres, AU - Rénia,Laurent, AU - Ng,Lisa F P, Y1 - 2012/12/03/ PY - 2012/12/5/entrez PY - 2012/12/5/pubmed PY - 2013/3/13/medline SP - 259 EP - 69 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 190 IS - 1 N2 - Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. Injury to the joint is believed to occur because of viral and host immune-mediated effects. However, the exact involvement of the different immune mediators in CHIKV-induced pathogenesis is unknown. In this study, we assessed the roles of T cells in primary CHIKV infection, virus replication and dissemination, and virus persistence, as well as in the mediation of disease severity in adult RAG2(-/-), CD4(-/-), CD8(-/-), and wild-type CHIKV C57BL/6J mice and in wild-type mice depleted of CD4(+) or CD8(+) T cells after Ab treatment. CHIKV-specific T cells in the spleen and footpad were investigated using IFN-γ ELISPOT. Interestingly, our results indicated that CHIKV-specific CD4(+), but not CD8(+), T cells are essential for the development of joint swelling without any effect on virus replication and dissemination. Infection in IFN-γ(-/-) mice demonstrated that pathogenic CD4(+) T cells do not mediate inflammation via an IFN-γ-mediated pathway. Taken together, these observations strongly indicate that mechanisms of joint pathology induced by CHIKV in mice resemble those in humans and differ from infections caused by other arthritogenic viruses, such as Ross River virus. SN - 1550-6606 UR - https://www.unboundmedicine.com/medline/citation/23209328/A_pathogenic_role_for_CD4+_T_cells_during_Chikungunya_virus_infection_in_mice_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=23209328 DB - PRIME DP - Unbound Medicine ER -