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Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice.
PLoS One. 2012; 7(11):e50089.Plos

Abstract

The allene oxide synthase (AOS) and hydroperoxide lyase (HPL) branches of the oxylipin pathway, which underlie the production of jasmonates and aldehydes, respectively, function in plant responses to a range of stresses. Regulatory crosstalk has been proposed to exist between these two signaling branches; however, there is no direct evidence of this. Here, we identified and characterized a jasmonic acid (JA) overproduction mutant, cea62, by screening a rice T-DNA insertion mutant library for lineages that constitutively express the AOS gene. Map-based cloning was used to identify the underlying gene as hydroperoxide lyase OsHPL3. HPL3 expression and the enzyme activity of its product, (E)-2-hexenal, were depleted in the cea62 mutant, which resulted in the dramatic overproduction of JA, the activation of JA signaling, and the emergence of the lesion mimic phenotype. A time-course analysis of lesion formation and of the induction of defense responsive genes in the cea62 mutant revealed that the activation of JA biosynthesis and signaling in cea62 was regulated in a developmental manner, as was OsHPL3 activity in the wild-type plant. Microarray analysis showed that the JA-governed defense response was greatly activated in cea62 and this plant exhibited enhanced resistance to the T1 strain of the bacterial blight pathogen Xanthomonasoryzaepvoryzae (Xoo). The wounding response was attenuated in cea62 plants during the early stages of development, but partially recovered when JA levels were elevated during the later stages. In contrast, the wounding response was not altered during the different developmental stages of wild-type plants. These findings suggest that these two branches of the oxylipin pathway exhibit crosstalk with regards to biosynthesis and signaling and cooperate with each other to function in diverse stress responses.

Authors+Show Affiliations

State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23209649

Citation

Liu, Xiaoqiang, et al. "Activation of the Jasmonic Acid Pathway By Depletion of the Hydroperoxide Lyase OsHPL3 Reveals Crosstalk Between the HPL and AOS Branches of the Oxylipin Pathway in Rice." PloS One, vol. 7, no. 11, 2012, pp. e50089.
Liu X, Li F, Tang J, et al. Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice. PLoS One. 2012;7(11):e50089.
Liu, X., Li, F., Tang, J., Wang, W., Zhang, F., Wang, G., Chu, J., Yan, C., Wang, T., Chu, C., & Li, C. (2012). Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice. PloS One, 7(11), e50089. https://doi.org/10.1371/journal.pone.0050089
Liu X, et al. Activation of the Jasmonic Acid Pathway By Depletion of the Hydroperoxide Lyase OsHPL3 Reveals Crosstalk Between the HPL and AOS Branches of the Oxylipin Pathway in Rice. PLoS One. 2012;7(11):e50089. PubMed PMID: 23209649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the jasmonic acid pathway by depletion of the hydroperoxide lyase OsHPL3 reveals crosstalk between the HPL and AOS branches of the oxylipin pathway in rice. AU - Liu,Xiaoqiang, AU - Li,Feng, AU - Tang,Jiuyou, AU - Wang,Weihong, AU - Zhang,Fengxia, AU - Wang,Guodong, AU - Chu,Jinfang, AU - Yan,Cunyu, AU - Wang,Taoqing, AU - Chu,Chengcai, AU - Li,Chuanyou, Y1 - 2012/11/29/ PY - 2012/09/03/received PY - 2012/10/15/accepted PY - 2012/12/5/entrez PY - 2012/12/5/pubmed PY - 2013/6/26/medline SP - e50089 EP - e50089 JF - PloS one JO - PLoS One VL - 7 IS - 11 N2 - The allene oxide synthase (AOS) and hydroperoxide lyase (HPL) branches of the oxylipin pathway, which underlie the production of jasmonates and aldehydes, respectively, function in plant responses to a range of stresses. Regulatory crosstalk has been proposed to exist between these two signaling branches; however, there is no direct evidence of this. Here, we identified and characterized a jasmonic acid (JA) overproduction mutant, cea62, by screening a rice T-DNA insertion mutant library for lineages that constitutively express the AOS gene. Map-based cloning was used to identify the underlying gene as hydroperoxide lyase OsHPL3. HPL3 expression and the enzyme activity of its product, (E)-2-hexenal, were depleted in the cea62 mutant, which resulted in the dramatic overproduction of JA, the activation of JA signaling, and the emergence of the lesion mimic phenotype. A time-course analysis of lesion formation and of the induction of defense responsive genes in the cea62 mutant revealed that the activation of JA biosynthesis and signaling in cea62 was regulated in a developmental manner, as was OsHPL3 activity in the wild-type plant. Microarray analysis showed that the JA-governed defense response was greatly activated in cea62 and this plant exhibited enhanced resistance to the T1 strain of the bacterial blight pathogen Xanthomonasoryzaepvoryzae (Xoo). The wounding response was attenuated in cea62 plants during the early stages of development, but partially recovered when JA levels were elevated during the later stages. In contrast, the wounding response was not altered during the different developmental stages of wild-type plants. These findings suggest that these two branches of the oxylipin pathway exhibit crosstalk with regards to biosynthesis and signaling and cooperate with each other to function in diverse stress responses. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23209649/Activation_of_the_jasmonic_acid_pathway_by_depletion_of_the_hydroperoxide_lyase_OsHPL3_reveals_crosstalk_between_the_HPL_and_AOS_branches_of_the_oxylipin_pathway_in_rice_ L2 - https://dx.plos.org/10.1371/journal.pone.0050089 DB - PRIME DP - Unbound Medicine ER -