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Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae.
J Integr Plant Biol. 2013 May; 55(5):473-83.JI

Abstract

A stable inherited rice spotted-leaf mutant HM47 derived from an EMS-induced IR64 mutant bank was identified. The mutant expressed hypersensitive response (HR)-like symptoms throughout its whole life from the first leaf to the flag leaf, without pathogen invasion. Initiation of the lesions was induced by light under natural summer field conditions. Expression of pathogenesis-related genes including PAL, PO-C1, POX22.3 and PBZ1 was enhanced significantly in association with cell death and accumulation of H2 O2 at and around the site of lesions in the mutant in contrast to that in the wild-type (WT). Disease reaction to Xanthomonas oryzae pv. oryzae from the Philippines and China showed that HM47 is a broad-spectrum disease-resistant mutant with enhanced resistance to multiple races of bacterial blight pathogens tested. An F2 progeny test showed that bacterial blight resistance to race HB-17 was co-segregated with the expression of lesions. Genetic analysis indicated that the spotted-leaf trait was controlled by a single recessive gene, tentatively named spl(HM47) , flanked by two insertion/deletion markers in a region of approximately 74 kb on the long arm of chromosome 4. Ten open reading frames are predicted, and all of them are expressed proteins. Isolation and validation of the putative genes are currently underway.

Authors+Show Affiliations

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23210861

Citation

Feng, Bao-Hua, et al. "Characterization and Genetic Analysis of a Novel Rice Spotted-leaf Mutant HM47 With Broad-spectrum Resistance to Xanthomonas Oryzae Pv. Oryzae." Journal of Integrative Plant Biology, vol. 55, no. 5, 2013, pp. 473-83.
Feng BH, Yang Y, Shi YF, et al. Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. J Integr Plant Biol. 2013;55(5):473-83.
Feng, B. H., Yang, Y., Shi, Y. F., Shen, H. C., Wang, H. M., Huang, Q. N., Xu, X., Lü, X. G., & Wu, J. L. (2013). Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. Journal of Integrative Plant Biology, 55(5), 473-83. https://doi.org/10.1111/jipb.12021
Feng BH, et al. Characterization and Genetic Analysis of a Novel Rice Spotted-leaf Mutant HM47 With Broad-spectrum Resistance to Xanthomonas Oryzae Pv. Oryzae. J Integr Plant Biol. 2013;55(5):473-83. PubMed PMID: 23210861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. AU - Feng,Bao-Hua, AU - Yang,Yang, AU - Shi,Yong-Feng, AU - Shen,Hai-Chao, AU - Wang,Hui-Mei, AU - Huang,Qi-Na, AU - Xu,Xia, AU - Lü,Xiang-Guang, AU - Wu,Jian-Li, Y1 - 2013/02/04/ PY - 2012/09/29/received PY - 2012/11/21/accepted PY - 2012/12/6/entrez PY - 2012/12/6/pubmed PY - 2013/10/23/medline SP - 473 EP - 83 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 55 IS - 5 N2 - A stable inherited rice spotted-leaf mutant HM47 derived from an EMS-induced IR64 mutant bank was identified. The mutant expressed hypersensitive response (HR)-like symptoms throughout its whole life from the first leaf to the flag leaf, without pathogen invasion. Initiation of the lesions was induced by light under natural summer field conditions. Expression of pathogenesis-related genes including PAL, PO-C1, POX22.3 and PBZ1 was enhanced significantly in association with cell death and accumulation of H2 O2 at and around the site of lesions in the mutant in contrast to that in the wild-type (WT). Disease reaction to Xanthomonas oryzae pv. oryzae from the Philippines and China showed that HM47 is a broad-spectrum disease-resistant mutant with enhanced resistance to multiple races of bacterial blight pathogens tested. An F2 progeny test showed that bacterial blight resistance to race HB-17 was co-segregated with the expression of lesions. Genetic analysis indicated that the spotted-leaf trait was controlled by a single recessive gene, tentatively named spl(HM47) , flanked by two insertion/deletion markers in a region of approximately 74 kb on the long arm of chromosome 4. Ten open reading frames are predicted, and all of them are expressed proteins. Isolation and validation of the putative genes are currently underway. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/23210861/Characterization_and_genetic_analysis_of_a_novel_rice_spotted_leaf_mutant_HM47_with_broad_spectrum_resistance_to_Xanthomonas_oryzae_pv__oryzae_ L2 - https://doi.org/10.1111/jipb.12021 DB - PRIME DP - Unbound Medicine ER -