Tags

Type your tag names separated by a space and hit enter

Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, interacts with gastric oxidative metabolism and enhances stress-induced gastric lesions.
J Physiol Pharmacol. 2012 Oct; 63(5):515-24.JP

Abstract

Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide (NO) synthase known to exert vasoconstriction of vascular bed. The elevation of ADMA has been considered as the cardiovascular risk factor associated with hyperlipidemia, hypercholesterolemia and metabolic syndrome. ADMA is produced by the action of dimethylarginine dimethylaminohydrolase (DDAH), which hydrolyzes ADMA to L-citrulline and dimethylamine. Previous studies have shown that endogenous NO plays an important role in the mechanism of gastric mucosal defense, but the role of ADMA in the pathogenesis of serious clinical entity, such as the acute gastric mucosal injury induced by stress has been little studied. In present study, we determined the effect of intragastric (i.g.) pretreatment with ADMA applied in graded doses ranging from 0.1 up to 20 mg/kg on gastric mucosal lesions induced by 3.5 h of water immersion and restraint stress (WRS). The number of gastric lesions was determined by planimetry and the gastric blood flow (GBF) was assessed by laser Doppler technique. The malondialdehyde and 4-hydroxynonenal (MDA+4-HNE) concentration, as an index of oxygen radical-lipid peroxidation was assessed in the gastric mucosa in rats exposed to WRS with or without ADMA administration. Proinflammatory cytokines IL-1β, TNF-α, superoxide dismutase (SOD) and glutathione peroxidase (GPx) mRNAs in the gastric mucosa and plasma levels of ADMA, IL-1β and TNF-α were analyzed by RT-PCR and ELISA, respectively. The exposure of rats to WRS for 3.5 h produced acute gastric lesions accompanied by a significant rise in the plasma ADMA levels and a significant fall in the GBF, an increase in MDA+4-HNE concentrations and the significant increase in the expression and release of IL-1β and TNF-α. The pretreatment with ADMA, applied i.g. 30 min before WRS dose-dependently, aggravated WRS damage and this effect was accompanied by a further significant fall in the GBF. The ADMA induced exacerbation of WRS lesions and the accompanying rise in the plasma ADMA levels and the fall in GBF were significantly attenuated by concurrent treatment with glyceryl trinitrate (GTN) (10 mg/kg i.g.) in the presence of ADMA. Administration of ADMA resulted in a significant decrease in the expression of SOD and GPx mRNAs and the up-regulation of mRNA for IL-1β and TNF-α followed by an increase in these plasma cytokine levels as compared to respective values observed in vehicle-pretreated animals. We conclude that 1) ADMA could be implicated in the mechanism of WRS-induced ulcerogenesis, 2) ADMA exacerbates WRS-induced gastric lesions due to enhancement in neutrophil dependent lipid peroxidation and overexpression and release of proinflammatory cytokines IL-1β and TNF-α and a potent depletion of antioxidative enzymes SOD and GPx expression and activity.

Authors+Show Affiliations

Department of Physiology Jagiellonian University Medical College, Cracow, Poland. skwiecien@cm-uj.krakow.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23211305

Citation

Kwiecien, S, et al. "Asymmetric Dimethylarginine, an Endogenous Inhibitor of Nitric Oxide Synthase, Interacts With Gastric Oxidative Metabolism and Enhances Stress-induced Gastric Lesions." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 63, no. 5, 2012, pp. 515-24.
Kwiecien S, Ptak-Belowska A, Krzysiek-Maczka G, et al. Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, interacts with gastric oxidative metabolism and enhances stress-induced gastric lesions. J Physiol Pharmacol. 2012;63(5):515-24.
Kwiecien, S., Ptak-Belowska, A., Krzysiek-Maczka, G., Targosz, A., Jasnos, K., Magierowski, M., Szczyrk, U., Brzozowski, B., Konturek, S. J., Konturek, P. C., & Brzozowski, T. (2012). Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, interacts with gastric oxidative metabolism and enhances stress-induced gastric lesions. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 63(5), 515-24.
Kwiecien S, et al. Asymmetric Dimethylarginine, an Endogenous Inhibitor of Nitric Oxide Synthase, Interacts With Gastric Oxidative Metabolism and Enhances Stress-induced Gastric Lesions. J Physiol Pharmacol. 2012;63(5):515-24. PubMed PMID: 23211305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, interacts with gastric oxidative metabolism and enhances stress-induced gastric lesions. AU - Kwiecien,S, AU - Ptak-Belowska,A, AU - Krzysiek-Maczka,G, AU - Targosz,A, AU - Jasnos,K, AU - Magierowski,M, AU - Szczyrk,U, AU - Brzozowski,B, AU - Konturek,S J, AU - Konturek,P C, AU - Brzozowski,T, PY - 2012/06/09/received PY - 2012/09/18/accepted PY - 2012/12/6/entrez PY - 2012/12/6/pubmed PY - 2013/5/23/medline SP - 515 EP - 24 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 63 IS - 5 N2 - Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide (NO) synthase known to exert vasoconstriction of vascular bed. The elevation of ADMA has been considered as the cardiovascular risk factor associated with hyperlipidemia, hypercholesterolemia and metabolic syndrome. ADMA is produced by the action of dimethylarginine dimethylaminohydrolase (DDAH), which hydrolyzes ADMA to L-citrulline and dimethylamine. Previous studies have shown that endogenous NO plays an important role in the mechanism of gastric mucosal defense, but the role of ADMA in the pathogenesis of serious clinical entity, such as the acute gastric mucosal injury induced by stress has been little studied. In present study, we determined the effect of intragastric (i.g.) pretreatment with ADMA applied in graded doses ranging from 0.1 up to 20 mg/kg on gastric mucosal lesions induced by 3.5 h of water immersion and restraint stress (WRS). The number of gastric lesions was determined by planimetry and the gastric blood flow (GBF) was assessed by laser Doppler technique. The malondialdehyde and 4-hydroxynonenal (MDA+4-HNE) concentration, as an index of oxygen radical-lipid peroxidation was assessed in the gastric mucosa in rats exposed to WRS with or without ADMA administration. Proinflammatory cytokines IL-1β, TNF-α, superoxide dismutase (SOD) and glutathione peroxidase (GPx) mRNAs in the gastric mucosa and plasma levels of ADMA, IL-1β and TNF-α were analyzed by RT-PCR and ELISA, respectively. The exposure of rats to WRS for 3.5 h produced acute gastric lesions accompanied by a significant rise in the plasma ADMA levels and a significant fall in the GBF, an increase in MDA+4-HNE concentrations and the significant increase in the expression and release of IL-1β and TNF-α. The pretreatment with ADMA, applied i.g. 30 min before WRS dose-dependently, aggravated WRS damage and this effect was accompanied by a further significant fall in the GBF. The ADMA induced exacerbation of WRS lesions and the accompanying rise in the plasma ADMA levels and the fall in GBF were significantly attenuated by concurrent treatment with glyceryl trinitrate (GTN) (10 mg/kg i.g.) in the presence of ADMA. Administration of ADMA resulted in a significant decrease in the expression of SOD and GPx mRNAs and the up-regulation of mRNA for IL-1β and TNF-α followed by an increase in these plasma cytokine levels as compared to respective values observed in vehicle-pretreated animals. We conclude that 1) ADMA could be implicated in the mechanism of WRS-induced ulcerogenesis, 2) ADMA exacerbates WRS-induced gastric lesions due to enhancement in neutrophil dependent lipid peroxidation and overexpression and release of proinflammatory cytokines IL-1β and TNF-α and a potent depletion of antioxidative enzymes SOD and GPx expression and activity. SN - 1899-1505 UR - https://www.unboundmedicine.com/medline/citation/23211305/Asymmetric_dimethylarginine_an_endogenous_inhibitor_of_nitric_oxide_synthase_interacts_with_gastric_oxidative_metabolism_and_enhances_stress_induced_gastric_lesions_ L2 - http://www.jpp.krakow.pl/journal/archive/10_12/pdf/515_10_12_article.pdf DB - PRIME DP - Unbound Medicine ER -