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Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE*3Leiden mice consuming a high-fat diet.
Br J Nutr. 2013 Jul 14; 110(1):77-85.BJ

Abstract

A high-fat diet disturbs the composition and function of the gut microbiota and generates local gut-associated and also systemic responses. Intestinal mast cells, for their part, secrete mediators which play a role in the orchestration of physiological and immunological functions of the intestine. Probiotic bacteria, again, help to maintain the homeostasis of the gut microbiota by protecting the gut epithelium and regulating the local immune system. In the present study, we explored the effects of two probiotic bacteria, Lactobacillus rhamnosus GG (GG) and Propionibacterium freudenreichii spp. shermanii JS (PJS), on high fat-fed ApoE*3Leiden mice by estimating the mast cell numbers and the immunoreactivity of TNF-α and IL-10 in the intestine, as well as plasma levels of several markers of inflammation and parameters of lipid metabolism. We found that mice that received GG and PJS exhibited significantly lower numbers of intestinal mast cells compared with control mice. PJS lowered intestinal immunoreactivity of TNF-α, while GG increased intestinal IL-10. PJS was also observed to lower the plasma levels of markers of inflammation including vascular cell adhesion molecule 1, and also the amount of gonadal adipose tissue. GG lowered alanine aminotransferase, a marker of hepatocellular activation. Collectively, these data demonstrate that probiotic GG and PJS tend to down-regulate both intestinal and systemic pro-inflammatory changes induced by a high-fat diet in this humanised mouse model.

Authors+Show Affiliations

Wihuri Research Institute, Kalliolinnantie 4, 00140 Helsinki, Finland. anna.oksaharju@helsinki.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23211714

Citation

Oksaharju, Anna, et al. "Effects of Probiotic Lactobacillus Rhamnosus GG and Propionibacterium Freudenreichii Ssp. Shermanii JS Supplementation On Intestinal and Systemic Markers of Inflammation in ApoE*3Leiden Mice Consuming a High-fat Diet." The British Journal of Nutrition, vol. 110, no. 1, 2013, pp. 77-85.
Oksaharju A, Kooistra T, Kleemann R, et al. Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE*3Leiden mice consuming a high-fat diet. Br J Nutr. 2013;110(1):77-85.
Oksaharju, A., Kooistra, T., Kleemann, R., van Duyvenvoorde, W., Miettinen, M., Lappalainen, J., Lindstedt, K. A., Kovanen, P. T., Korpela, R., & Kekkonen, R. A. (2013). Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE*3Leiden mice consuming a high-fat diet. The British Journal of Nutrition, 110(1), 77-85. https://doi.org/10.1017/S0007114512004801
Oksaharju A, et al. Effects of Probiotic Lactobacillus Rhamnosus GG and Propionibacterium Freudenreichii Ssp. Shermanii JS Supplementation On Intestinal and Systemic Markers of Inflammation in ApoE*3Leiden Mice Consuming a High-fat Diet. Br J Nutr. 2013 Jul 14;110(1):77-85. PubMed PMID: 23211714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE*3Leiden mice consuming a high-fat diet. AU - Oksaharju,Anna, AU - Kooistra,Teake, AU - Kleemann,Robert, AU - van Duyvenvoorde,Wim, AU - Miettinen,Minja, AU - Lappalainen,Jani, AU - Lindstedt,Ken A, AU - Kovanen,Petri T, AU - Korpela,Riitta, AU - Kekkonen,Riina A, Y1 - 2012/12/05/ PY - 2012/12/6/entrez PY - 2012/12/6/pubmed PY - 2013/8/6/medline SP - 77 EP - 85 JF - The British journal of nutrition JO - Br J Nutr VL - 110 IS - 1 N2 - A high-fat diet disturbs the composition and function of the gut microbiota and generates local gut-associated and also systemic responses. Intestinal mast cells, for their part, secrete mediators which play a role in the orchestration of physiological and immunological functions of the intestine. Probiotic bacteria, again, help to maintain the homeostasis of the gut microbiota by protecting the gut epithelium and regulating the local immune system. In the present study, we explored the effects of two probiotic bacteria, Lactobacillus rhamnosus GG (GG) and Propionibacterium freudenreichii spp. shermanii JS (PJS), on high fat-fed ApoE*3Leiden mice by estimating the mast cell numbers and the immunoreactivity of TNF-α and IL-10 in the intestine, as well as plasma levels of several markers of inflammation and parameters of lipid metabolism. We found that mice that received GG and PJS exhibited significantly lower numbers of intestinal mast cells compared with control mice. PJS lowered intestinal immunoreactivity of TNF-α, while GG increased intestinal IL-10. PJS was also observed to lower the plasma levels of markers of inflammation including vascular cell adhesion molecule 1, and also the amount of gonadal adipose tissue. GG lowered alanine aminotransferase, a marker of hepatocellular activation. Collectively, these data demonstrate that probiotic GG and PJS tend to down-regulate both intestinal and systemic pro-inflammatory changes induced by a high-fat diet in this humanised mouse model. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/23211714/Effects_of_probiotic_Lactobacillus_rhamnosus_GG_and_Propionibacterium_freudenreichii_ssp__shermanii_JS_supplementation_on_intestinal_and_systemic_markers_of_inflammation_in_ApoE_3Leiden_mice_consuming_a_high_fat_diet_ L2 - https://www.cambridge.org/core/product/identifier/S0007114512004801/type/journal_article DB - PRIME DP - Unbound Medicine ER -