[Study on the HPLC-DAD fingerprint chromatograms of Radix Rubiae from different areas in China].Zhong Yao Cai. 2012 May; 35(5):709-11.ZY
To establish the HPLC-DAD fingerprint of Radix Rubiae and provide a quality control method for it.
The method HPLC-DAD was performed on a YMC C,18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase, flow rate at 1.0 mL/min, detection wavelength at 280 mm, and the column temperature at 25 degrees C, and the analysis time was 60 min.
The average similarity of the fnigerprint chromatograms was over 0.94 in 6 batches samples of different areas in China.
This method can be applied for the determination of Radix Rubiae from different areas.