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[Research on the changes of IL-1 receptor and TNF-alpha receptor in rats with cerebral ischemia reperfusion and the chronergy of acupuncture intervention].
Zhongguo Zhen Jiu. 2012 Nov; 32(11):1012-8.ZZ

Abstract

OBJECTIVE

To explore the intervention timing of acupuncture in treatment of cerebral infarction and the relationship of cerebral ischemia reperfusion injury with inflammatory cytokine receptor.

METHODS

One hundred and ten male healthy Wistar rats were randomly divided into a normal group (n=10), a sham operation group (n=10), a model group (n=10), an acupuncture at non-acupoint group (non-acupoint group, n=40), an acupuncture with regaining consciousness method group (regaining consciousness group, n=40). Four subgroups were set up 1 h ischemia reperfusion in 1 h group, 3 h group, 6 h group, 12 h group in the two acupuncture groups, 10 rats in each subgroup. Two acupuncture groups were treated with acupuncture at four time points (1 h, 3 h, 6 h and 12 h after ischemia reperfusion), and "Shuigou" (GV 26) and "Neiguan" (PC 6) were selected in regaining consciousness group, and the non-acupoints below the bilateral costal region were selected in non-acupoint group. At the corresponding time point, the tissues of the brain were removed and interleukin1 receptor (IL-1RI) and tumor necrosis factor receptor (TNFR-I) mRNA and protein changes were detected by using real-time quantitative polymerase chain reaction and immunoblot assay.

RESULTS

The expression of IL-1RI and TNFR-I mRNA and protein in the model group were significantly higher than that in normal group, sham operation group, regaining consciousness group and non-acupoint group (P<0.01, P<0.05). The expression of IL-1RI and TNFR-I mRNA and protein in regaining consciousness group was weakest at 3 h after reperfusion followed successively by 6 h, 1 h, 12 h, and there was no significantly change of IL-1RI and TNFR-I mRNA and protein expression in non-acupoint group among different timing points, but which was decreased as compared with those in the model group at the same time point (all P<0.05).

CONCLUSION

Acupuncture can reduce the expression of IL-1RI and TNFR-I mRNA and protein in rats with cerebral ischemia reperfusion, inhibit the excessive expression of proinflammatory cytokine receptor, block apoptosis signal transduction and extend time window for treatment of cerebral ischemia, so as to play the protective effect for brain. Within 3 h of ischemia is the best time for intervention of acupuncture treatment.

Authors+Show Affiliations

Center of Acupuncture and Encephalopathy, The Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China. wzk21cn@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23213990

Citation

Wang, Zhan-Kui, et al. "[Research On the Changes of IL-1 Receptor and TNF-alpha Receptor in Rats With Cerebral Ischemia Reperfusion and the Chronergy of Acupuncture Intervention]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 32, no. 11, 2012, pp. 1012-8.
Wang ZK, Ni GX, Liu K, et al. [Research on the changes of IL-1 receptor and TNF-alpha receptor in rats with cerebral ischemia reperfusion and the chronergy of acupuncture intervention]. Zhongguo Zhen Jiu. 2012;32(11):1012-8.
Wang, Z. K., Ni, G. X., Liu, K., Xiao, Z. X., Yang, B. W., Wang, J., & Wang, S. (2012). [Research on the changes of IL-1 receptor and TNF-alpha receptor in rats with cerebral ischemia reperfusion and the chronergy of acupuncture intervention]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 32(11), 1012-8.
Wang ZK, et al. [Research On the Changes of IL-1 Receptor and TNF-alpha Receptor in Rats With Cerebral Ischemia Reperfusion and the Chronergy of Acupuncture Intervention]. Zhongguo Zhen Jiu. 2012;32(11):1012-8. PubMed PMID: 23213990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Research on the changes of IL-1 receptor and TNF-alpha receptor in rats with cerebral ischemia reperfusion and the chronergy of acupuncture intervention]. AU - Wang,Zhan-Kui, AU - Ni,Guang-Xia, AU - Liu,Kun, AU - Xiao,Zhen-Xin, AU - Yang,Bao-Wang, AU - Wang,Jing, AU - Wang,Shu, PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2013/1/9/medline SP - 1012 EP - 8 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 32 IS - 11 N2 - OBJECTIVE: To explore the intervention timing of acupuncture in treatment of cerebral infarction and the relationship of cerebral ischemia reperfusion injury with inflammatory cytokine receptor. METHODS: One hundred and ten male healthy Wistar rats were randomly divided into a normal group (n=10), a sham operation group (n=10), a model group (n=10), an acupuncture at non-acupoint group (non-acupoint group, n=40), an acupuncture with regaining consciousness method group (regaining consciousness group, n=40). Four subgroups were set up 1 h ischemia reperfusion in 1 h group, 3 h group, 6 h group, 12 h group in the two acupuncture groups, 10 rats in each subgroup. Two acupuncture groups were treated with acupuncture at four time points (1 h, 3 h, 6 h and 12 h after ischemia reperfusion), and "Shuigou" (GV 26) and "Neiguan" (PC 6) were selected in regaining consciousness group, and the non-acupoints below the bilateral costal region were selected in non-acupoint group. At the corresponding time point, the tissues of the brain were removed and interleukin1 receptor (IL-1RI) and tumor necrosis factor receptor (TNFR-I) mRNA and protein changes were detected by using real-time quantitative polymerase chain reaction and immunoblot assay. RESULTS: The expression of IL-1RI and TNFR-I mRNA and protein in the model group were significantly higher than that in normal group, sham operation group, regaining consciousness group and non-acupoint group (P<0.01, P<0.05). The expression of IL-1RI and TNFR-I mRNA and protein in regaining consciousness group was weakest at 3 h after reperfusion followed successively by 6 h, 1 h, 12 h, and there was no significantly change of IL-1RI and TNFR-I mRNA and protein expression in non-acupoint group among different timing points, but which was decreased as compared with those in the model group at the same time point (all P<0.05). CONCLUSION: Acupuncture can reduce the expression of IL-1RI and TNFR-I mRNA and protein in rats with cerebral ischemia reperfusion, inhibit the excessive expression of proinflammatory cytokine receptor, block apoptosis signal transduction and extend time window for treatment of cerebral ischemia, so as to play the protective effect for brain. Within 3 h of ischemia is the best time for intervention of acupuncture treatment. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/23213990/[Research_on_the_changes_of_IL_1_receptor_and_TNF_alpha_receptor_in_rats_with_cerebral_ischemia_reperfusion_and_the_chronergy_of_acupuncture_intervention]_ L2 - https://medlineplus.gov/acupuncture.html DB - PRIME DP - Unbound Medicine ER -