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Evaluation of the effects of a high dose of erythropoietin-beta on early endotoxemia using a rat model.
Adv Clin Exp Med. 2012 May-Jun; 21(3):321-9.AC

Abstract

BACKGROUND

Endotoxins can cause serious organ damage and death by triggering the secretion of pro-inflammatory cytokines such as TNF-alpha, IL-6 and IL-1beta in bacterial infections.

OBJECTIVES

The goal of this study was to evaluate the effects of a high dose (3000 U/kg) of erythropoietin-beta (EPO) on inflammatory cytokine levels, renal function and histological changes during the early period of Lipopolysaccharide (LPS)-induced endotoxemia using a rat model.

MATERIAL AND METHODS

Male Sprague Dawley (350-400 g) rats were randomized into 3 groups: Control group (n = 7); LPS group (received 20 mcg/kg LPS through intraperitoneal (i.p.) injection (n = 7); LPS+EPO group (received 3000 U/kg, ip 30 minutes before LPS administration (n = 7). Four hours after the administration of LPS, kidney tissue and serum samples were collected. Kidney function parameters, TNF-alpha, IL-6, IL-1beta, C reactive protein (CRP) and complete blood counts (CBC) were measured. The severity of renal tubular injury and caspase-9 immunoreactive cells was expressed as a percentage.

RESULTS

Serum levels of urea, creatinine, TNF-alpha, IL-6 and IL-1beta were significantly increased in the LPS group (p < 0.0001 - p = 0.04) and were lower in LPS+EPO group (p < 0.0001, p = 0.01, p = 0.02, p = 01 and p < 0.0001, respectively). Pretreatment with EPO significantly increased platelet counts (p = 0.00) and decreased white blood cell counts (p = 0.02). The renal tubular injury percentage was significantly higher in the LPS group than in the control and LPS+EPO groups (p = 0.002, p = 0.003, and p = 0.005, respectively) and caspase-9 expression was lower in the LPS+EPO and control groups than in the LPS group.

CONCLUSIONS

EPO might have renoprotective effects against the inflammatory process and cell apoptosis during endotoxemia.

Authors+Show Affiliations

Department of Nephrology, Yeditepe University Hospital, Istanbul, Turkey. zeheren@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23214195

Citation

Eren, Zehra, et al. "Evaluation of the Effects of a High Dose of Erythropoietin-beta On Early Endotoxemia Using a Rat Model." Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University, vol. 21, no. 3, 2012, pp. 321-9.
Eren Z, Coban J, Ekinci ID, et al. Evaluation of the effects of a high dose of erythropoietin-beta on early endotoxemia using a rat model. Adv Clin Exp Med. 2012;21(3):321-9.
Eren, Z., Coban, J., Ekinci, I. D., Kaspar, C., & Kantarci, G. (2012). Evaluation of the effects of a high dose of erythropoietin-beta on early endotoxemia using a rat model. Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University, 21(3), 321-9.
Eren Z, et al. Evaluation of the Effects of a High Dose of Erythropoietin-beta On Early Endotoxemia Using a Rat Model. Adv Clin Exp Med. 2012 May-Jun;21(3):321-9. PubMed PMID: 23214195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the effects of a high dose of erythropoietin-beta on early endotoxemia using a rat model. AU - Eren,Zehra, AU - Coban,Jale, AU - Ekinci,Işin D, AU - Kaspar,Cigdem, AU - Kantarci,Gülçlin, PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2013/1/5/medline SP - 321 EP - 9 JF - Advances in clinical and experimental medicine : official organ Wroclaw Medical University JO - Adv Clin Exp Med VL - 21 IS - 3 N2 - BACKGROUND: Endotoxins can cause serious organ damage and death by triggering the secretion of pro-inflammatory cytokines such as TNF-alpha, IL-6 and IL-1beta in bacterial infections. OBJECTIVES: The goal of this study was to evaluate the effects of a high dose (3000 U/kg) of erythropoietin-beta (EPO) on inflammatory cytokine levels, renal function and histological changes during the early period of Lipopolysaccharide (LPS)-induced endotoxemia using a rat model. MATERIAL AND METHODS: Male Sprague Dawley (350-400 g) rats were randomized into 3 groups: Control group (n = 7); LPS group (received 20 mcg/kg LPS through intraperitoneal (i.p.) injection (n = 7); LPS+EPO group (received 3000 U/kg, ip 30 minutes before LPS administration (n = 7). Four hours after the administration of LPS, kidney tissue and serum samples were collected. Kidney function parameters, TNF-alpha, IL-6, IL-1beta, C reactive protein (CRP) and complete blood counts (CBC) were measured. The severity of renal tubular injury and caspase-9 immunoreactive cells was expressed as a percentage. RESULTS: Serum levels of urea, creatinine, TNF-alpha, IL-6 and IL-1beta were significantly increased in the LPS group (p < 0.0001 - p = 0.04) and were lower in LPS+EPO group (p < 0.0001, p = 0.01, p = 0.02, p = 01 and p < 0.0001, respectively). Pretreatment with EPO significantly increased platelet counts (p = 0.00) and decreased white blood cell counts (p = 0.02). The renal tubular injury percentage was significantly higher in the LPS group than in the control and LPS+EPO groups (p = 0.002, p = 0.003, and p = 0.005, respectively) and caspase-9 expression was lower in the LPS+EPO and control groups than in the LPS group. CONCLUSIONS: EPO might have renoprotective effects against the inflammatory process and cell apoptosis during endotoxemia. SN - 1899-5276 UR - https://www.unboundmedicine.com/medline/citation/23214195/Evaluation_of_the_effects_of_a_high_dose_of_erythropoietin_beta_on_early_endotoxemia_using_a_rat_model_ L2 - http://www.advances.am.wroc.pl/pdf/2012/21/3/321.pdf DB - PRIME DP - Unbound Medicine ER -