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Acute effect of β-sitosterol on calcium uptake mediates anti-inflammatory effect in murine activated neutrophils.
J Pharm Pharmacol. 2013 Jan; 65(1):115-22.JP

Abstract

OBJECTIVES

To evaluate the effect of β-sitosterol on ⁴⁵Ca²⁺ uptake in activated murine neutrophils, and upon myeloperoxidase and adenosine deaminase activity, and interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) levels, in carrageenan-induced inflammation in the mouse air pouch model.

METHODS

Dried Esenbeckia leiocarpa bark was macerated and extracted resulting in a crude hydroalcoholic extract (CHE) that was partitioned to obtain an alkaloid fraction. The alkaloid was then partitioned in polar and nonpolar subfractions. β-Sitosterol was isolated from the nonpolar subfraction and identified by comparison with the literature. The effect of β-sitosterol on ⁴⁵Ca²⁺ uptake in activated murine neutrophils, and upon myeloperoxidase and adenosine deaminase activity, IL-1β and TNF-α levels in carrageenan-induced inflammation in mice were evaluated.

KEY FINDINGS

β-Sitosterol promoted a time- and dose-dependent increase of the calcium uptake in activated neutrophils that was promptly reversed by nifedipine, BAPTA-AM, LY294002, and colchicine. β-Sitosterol inhibited myeloperoxidase and adenosine deaminase activity, and IL-1β and TNF-α levels.

CONCLUSIONS

β-Sitosterol inhibited either myeloperoxidase and adenosine deaminase activity or IL-1β and TNF-α levels. This effect seemed to be mediated by the calcium uptake in activated neutrophils in a time- and concentration-dependent manner through L-type voltage dependent calcium channels, intracellular calcium, phosphoinositide kinase-3, and microtubule modulation.

Authors+Show Affiliations

Department of Clinical Analysis, Centre of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23215694

Citation

Liz, Rafael, et al. "Acute Effect of Β-sitosterol On Calcium Uptake Mediates Anti-inflammatory Effect in Murine Activated Neutrophils." The Journal of Pharmacy and Pharmacology, vol. 65, no. 1, 2013, pp. 115-22.
Liz R, Zanatta L, dos Reis GO, et al. Acute effect of β-sitosterol on calcium uptake mediates anti-inflammatory effect in murine activated neutrophils. J Pharm Pharmacol. 2013;65(1):115-22.
Liz, R., Zanatta, L., dos Reis, G. O., Horst, H., Pizzolatti, M. G., Silva, F. R., & Fröde, T. S. (2013). Acute effect of β-sitosterol on calcium uptake mediates anti-inflammatory effect in murine activated neutrophils. The Journal of Pharmacy and Pharmacology, 65(1), 115-22. https://doi.org/10.1111/j.2042-7158.2012.01568.x
Liz R, et al. Acute Effect of Β-sitosterol On Calcium Uptake Mediates Anti-inflammatory Effect in Murine Activated Neutrophils. J Pharm Pharmacol. 2013;65(1):115-22. PubMed PMID: 23215694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute effect of β-sitosterol on calcium uptake mediates anti-inflammatory effect in murine activated neutrophils. AU - Liz,Rafael, AU - Zanatta,Leila, AU - dos Reis,Gustavo Oliveira, AU - Horst,Heros, AU - Pizzolatti,Moacir Geraldo, AU - Silva,Fátima Regina Mena Barreto, AU - Fröde,Tânia Silvia, Y1 - 2012/10/11/ PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2013/5/15/medline SP - 115 EP - 22 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 65 IS - 1 N2 - OBJECTIVES: To evaluate the effect of β-sitosterol on ⁴⁵Ca²⁺ uptake in activated murine neutrophils, and upon myeloperoxidase and adenosine deaminase activity, and interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) levels, in carrageenan-induced inflammation in the mouse air pouch model. METHODS: Dried Esenbeckia leiocarpa bark was macerated and extracted resulting in a crude hydroalcoholic extract (CHE) that was partitioned to obtain an alkaloid fraction. The alkaloid was then partitioned in polar and nonpolar subfractions. β-Sitosterol was isolated from the nonpolar subfraction and identified by comparison with the literature. The effect of β-sitosterol on ⁴⁵Ca²⁺ uptake in activated murine neutrophils, and upon myeloperoxidase and adenosine deaminase activity, IL-1β and TNF-α levels in carrageenan-induced inflammation in mice were evaluated. KEY FINDINGS: β-Sitosterol promoted a time- and dose-dependent increase of the calcium uptake in activated neutrophils that was promptly reversed by nifedipine, BAPTA-AM, LY294002, and colchicine. β-Sitosterol inhibited myeloperoxidase and adenosine deaminase activity, and IL-1β and TNF-α levels. CONCLUSIONS: β-Sitosterol inhibited either myeloperoxidase and adenosine deaminase activity or IL-1β and TNF-α levels. This effect seemed to be mediated by the calcium uptake in activated neutrophils in a time- and concentration-dependent manner through L-type voltage dependent calcium channels, intracellular calcium, phosphoinositide kinase-3, and microtubule modulation. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/23215694/Acute_effect_of_β_sitosterol_on_calcium_uptake_mediates_anti_inflammatory_effect_in_murine_activated_neutrophils_ L2 - https://doi.org/10.1111/j.2042-7158.2012.01568.x DB - PRIME DP - Unbound Medicine ER -