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Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles.
Br J Pharmacol. 2013 Apr; 168(8):1902-10.BJ

Abstract

BACKGROUND AND PURPOSE

Hydrogen sulphide (H2S) is an endogenous gasotransmitter. Although it has been shown to elicit responses in vascular and other smooth muscle preparations, a role for endogenously produced H2S in mediating airway tone has yet to be demonstrated. Therefore, the aim of this study was to determine whether H2S is produced within the airways and to determine the functional effect on airway tone.

EXPERIMENTAL APPROACH

Small peripheral airways (<5 mm in diameter) from porcine lungs were set up in isolated tissue baths, pre-contracted with the muscarinic agonist carbachol, and then exposed to either the H2S donor sodium hydrosulphide (NaHS), or the precursor L-cysteine. H2S production from L-cysteine or 3-mercaptopyruvate in tissue homogenates was measured by the methylene blue assay. Expression of the H2S-synthesizing enzymes cystathionine β-synthase (CBS), cystathionine γ lyase (CSE) and 3-mercaptopyruvate sulphurtransferase (3-MST) were measured by Western blotting.

KEY RESULTS

NaHS caused a large relaxation of the airways, which was inhibited partially by pre-contraction with KCl or exposure to tetraethylammonium, but not glibenclamide, paxilline or 4-aminopyridine. L-cysteine also caused a relaxation of the airways which was inhibited by the CBS inhibitor aminooxyacetic acid. Tissue homogenates from airways exposed to L-cysteine or 3-mercaptopyruvate in vitro showed a significant production of H2S. Western blotting demonstrated immunoreactivity to CBS, CSE and 3-MST enzymes in the airways.

CONCLUSIONS AND IMPLICATIONS

These data demonstrate that H2S can be produced endogenously within porcine airways causing relaxation. The mechanism of relaxation depends, in part, on K(+) channel activity.

Authors+Show Affiliations

Cardiovascular Research Group, School of Biomedical Sciences, University of Nottingham Medical School, Nottingham, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23215842

Citation

Rashid, S, et al. "Hydrogen Sulphide-induced Relaxation of Porcine Peripheral Bronchioles." British Journal of Pharmacology, vol. 168, no. 8, 2013, pp. 1902-10.
Rashid S, Heer JK, Garle MJ, et al. Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles. Br J Pharmacol. 2013;168(8):1902-10.
Rashid, S., Heer, J. K., Garle, M. J., Alexander, S. P., & Roberts, R. E. (2013). Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles. British Journal of Pharmacology, 168(8), 1902-10. https://doi.org/10.1111/bph.12084
Rashid S, et al. Hydrogen Sulphide-induced Relaxation of Porcine Peripheral Bronchioles. Br J Pharmacol. 2013;168(8):1902-10. PubMed PMID: 23215842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles. AU - Rashid,S, AU - Heer,J K, AU - Garle,M J, AU - Alexander,S P H, AU - Roberts,R E, PY - 2012/07/06/received PY - 2012/10/29/revised PY - 2012/11/26/accepted PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2015/7/15/medline SP - 1902 EP - 10 JF - British journal of pharmacology JO - Br J Pharmacol VL - 168 IS - 8 N2 - BACKGROUND AND PURPOSE: Hydrogen sulphide (H2S) is an endogenous gasotransmitter. Although it has been shown to elicit responses in vascular and other smooth muscle preparations, a role for endogenously produced H2S in mediating airway tone has yet to be demonstrated. Therefore, the aim of this study was to determine whether H2S is produced within the airways and to determine the functional effect on airway tone. EXPERIMENTAL APPROACH: Small peripheral airways (<5 mm in diameter) from porcine lungs were set up in isolated tissue baths, pre-contracted with the muscarinic agonist carbachol, and then exposed to either the H2S donor sodium hydrosulphide (NaHS), or the precursor L-cysteine. H2S production from L-cysteine or 3-mercaptopyruvate in tissue homogenates was measured by the methylene blue assay. Expression of the H2S-synthesizing enzymes cystathionine β-synthase (CBS), cystathionine γ lyase (CSE) and 3-mercaptopyruvate sulphurtransferase (3-MST) were measured by Western blotting. KEY RESULTS: NaHS caused a large relaxation of the airways, which was inhibited partially by pre-contraction with KCl or exposure to tetraethylammonium, but not glibenclamide, paxilline or 4-aminopyridine. L-cysteine also caused a relaxation of the airways which was inhibited by the CBS inhibitor aminooxyacetic acid. Tissue homogenates from airways exposed to L-cysteine or 3-mercaptopyruvate in vitro showed a significant production of H2S. Western blotting demonstrated immunoreactivity to CBS, CSE and 3-MST enzymes in the airways. CONCLUSIONS AND IMPLICATIONS: These data demonstrate that H2S can be produced endogenously within porcine airways causing relaxation. The mechanism of relaxation depends, in part, on K(+) channel activity. SN - 1476-5381 UR - https://www.unboundmedicine.com/medline/citation/23215842/Hydrogen_sulphide_induced_relaxation_of_porcine_peripheral_bronchioles_ L2 - https://doi.org/10.1111/bph.12084 DB - PRIME DP - Unbound Medicine ER -