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Analysis of the NMI01 marker for a population database of cannabis seeds.
J Forensic Sci. 2013 Jan; 58 Suppl 1:S176-82.JF

Abstract

We have analyzed the distribution of genotypes at a single hexanucleotide short tandem repeat (STR) locus in a Cannabis sativa seed database along with seed-packaging information. This STR locus is defined by the polymerase chain reaction amplification primers CS1F and CS1R and is referred to as NMI01 (for National Marijuana Initiative) in our study. The population database consists of seed seizures of two categories: seed samples from labeled and unlabeled packages regarding seed bank source. Of a population database of 93 processed seeds including 12 labeled Cannabis varieties, the observed genotypes generated from single seeds exhibited between one and three peaks (potentially six alleles if in homozygous state). The total number of observed genotypes was 54 making this marker highly specific and highly individualizing even among seeds of common lineage. Cluster analysis associated many but not all of the handwritten labeled seed varieties tested to date as well as the National Park seizure to our known reference database containing Mr. Nice Seedbank and Sensi Seeds commercially packaged reference samples.

Authors+Show Affiliations

Forensic Science Department, Henry C. Lee College of Criminal Justice & Forensic Sciences, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23216136

Citation

Shirley, Nicholas, et al. "Analysis of the NMI01 Marker for a Population Database of Cannabis Seeds." Journal of Forensic Sciences, vol. 58 Suppl 1, 2013, pp. S176-82.
Shirley N, Allgeier L, Lanier T, et al. Analysis of the NMI01 marker for a population database of cannabis seeds. J Forensic Sci. 2013;58 Suppl 1:S176-82.
Shirley, N., Allgeier, L., Lanier, T., & Coyle, H. M. (2013). Analysis of the NMI01 marker for a population database of cannabis seeds. Journal of Forensic Sciences, 58 Suppl 1, S176-82. https://doi.org/10.1111/1556-4029.12005
Shirley N, et al. Analysis of the NMI01 Marker for a Population Database of Cannabis Seeds. J Forensic Sci. 2013;58 Suppl 1:S176-82. PubMed PMID: 23216136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the NMI01 marker for a population database of cannabis seeds. AU - Shirley,Nicholas, AU - Allgeier,Lindsay, AU - Lanier,Tommy, AU - Coyle,Heather Miller, Y1 - 2012/12/06/ PY - 2011/08/15/received PY - 2011/12/13/revised PY - 2011/12/28/accepted PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2013/7/17/medline SP - S176 EP - 82 JF - Journal of forensic sciences JO - J Forensic Sci VL - 58 Suppl 1 N2 - We have analyzed the distribution of genotypes at a single hexanucleotide short tandem repeat (STR) locus in a Cannabis sativa seed database along with seed-packaging information. This STR locus is defined by the polymerase chain reaction amplification primers CS1F and CS1R and is referred to as NMI01 (for National Marijuana Initiative) in our study. The population database consists of seed seizures of two categories: seed samples from labeled and unlabeled packages regarding seed bank source. Of a population database of 93 processed seeds including 12 labeled Cannabis varieties, the observed genotypes generated from single seeds exhibited between one and three peaks (potentially six alleles if in homozygous state). The total number of observed genotypes was 54 making this marker highly specific and highly individualizing even among seeds of common lineage. Cluster analysis associated many but not all of the handwritten labeled seed varieties tested to date as well as the National Park seizure to our known reference database containing Mr. Nice Seedbank and Sensi Seeds commercially packaged reference samples. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/23216136/Analysis_of_the_NMI01_marker_for_a_population_database_of_cannabis_seeds_ L2 - https://doi.org/10.1111/1556-4029.12005 DB - PRIME DP - Unbound Medicine ER -