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Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals.
J Appl Microbiol. 2013 Mar; 114(3):695-702.JA

Abstract

AIMS

To investigate plasmid-mediated fosfomycin resistance related to fosA3 in Escherichia coli isolates collected from different animals in Hong Kong, China, 2008-2010.

METHODS AND RESULTS

In total, 2106 faecal specimens from 210 cattle, 214 pigs, 460 chickens, 398 stray cats, 368 stray dogs and 456 wild rodents were cultured. The faecal colonization rates of fosfomycin-resistant E. coli were as follows: 11.2% in pigs, 8.6% in cattle, 7.3% in chickens, 2.4% in dogs, 0.8% in cats and 1.5% in rodents. The cultures yielded 1693 isolates of which 831 were extended-spectrum β-lactamases (ESBL) producers. Fosfomycin-resistant isolates were more likely than fosfomycin-susceptible isolates to be producers of ESBL and to have resistance to chloramphenicol, ciprofloxacin, cotrimoxazole, gentamicin and tetracycline. Of the 101 fosfomycin-resistant isolates, 97 (96.0%) isolates were fosA3 positive and 94 (93.1%) were bla(CTX) (-M) positive. PCR mapping showed that the fosA3-containing regions were flanked by IS26, both upstream and downstream in 81 (83.5%) isolates, and by an upstream bla(CTX-M-14) -containing transposon-like structure (ΔISEcp1-bla(CTX-M-14) -ΔIS903 or ISEcp1-IS10 -bla(CTX-M-14) -ΔIS903) and a downstream IS26 in 14 (14.4%) isolates. For the remaining two isolates, fosA3 was flanked by a downstream IS26 but the upstream part cannot be defined. In a random subset of 18 isolates, fosA3 was carried on transferable plasmids with sizes of 50-200 kb and the following replicons: F2:A-B- (n = 3), F16:A1:B- (n = 2), F24:A-B- (n = 1), N (n = 1), B/O (n = 1) and untypeable (n = 3).

SIGNIFICANCE AND IMPACT OF THE STUDY

This study demonstrates the emergence of fosA3-mediated fosfomycin resistance among multidrug-resistant E. coli isolates from various animals. IS26 transposon-like structures might be the main vehicles for dissemination of fosA3.

Authors+Show Affiliations

Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong, China. plho@hkucc.hku.hkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23216653

Citation

Ho, P L., et al. "Dissemination of Plasmid-mediated Fosfomycin Resistance fosA3 Among Multidrug-resistant Escherichia Coli From Livestock and Other Animals." Journal of Applied Microbiology, vol. 114, no. 3, 2013, pp. 695-702.
Ho PL, Chan J, Lo WU, et al. Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals. J Appl Microbiol. 2013;114(3):695-702.
Ho, P. L., Chan, J., Lo, W. U., Law, P. Y., Li, Z., Lai, E. L., & Chow, K. H. (2013). Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals. Journal of Applied Microbiology, 114(3), 695-702. https://doi.org/10.1111/jam.12099
Ho PL, et al. Dissemination of Plasmid-mediated Fosfomycin Resistance fosA3 Among Multidrug-resistant Escherichia Coli From Livestock and Other Animals. J Appl Microbiol. 2013;114(3):695-702. PubMed PMID: 23216653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals. AU - Ho,P L, AU - Chan,J, AU - Lo,W U, AU - Law,P Y, AU - Li,Z, AU - Lai,E L, AU - Chow,K H, Y1 - 2012/12/27/ PY - 2012/09/03/received PY - 2012/11/29/revised PY - 2012/12/04/accepted PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2014/2/1/medline SP - 695 EP - 702 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 114 IS - 3 N2 - AIMS: To investigate plasmid-mediated fosfomycin resistance related to fosA3 in Escherichia coli isolates collected from different animals in Hong Kong, China, 2008-2010. METHODS AND RESULTS: In total, 2106 faecal specimens from 210 cattle, 214 pigs, 460 chickens, 398 stray cats, 368 stray dogs and 456 wild rodents were cultured. The faecal colonization rates of fosfomycin-resistant E. coli were as follows: 11.2% in pigs, 8.6% in cattle, 7.3% in chickens, 2.4% in dogs, 0.8% in cats and 1.5% in rodents. The cultures yielded 1693 isolates of which 831 were extended-spectrum β-lactamases (ESBL) producers. Fosfomycin-resistant isolates were more likely than fosfomycin-susceptible isolates to be producers of ESBL and to have resistance to chloramphenicol, ciprofloxacin, cotrimoxazole, gentamicin and tetracycline. Of the 101 fosfomycin-resistant isolates, 97 (96.0%) isolates were fosA3 positive and 94 (93.1%) were bla(CTX) (-M) positive. PCR mapping showed that the fosA3-containing regions were flanked by IS26, both upstream and downstream in 81 (83.5%) isolates, and by an upstream bla(CTX-M-14) -containing transposon-like structure (ΔISEcp1-bla(CTX-M-14) -ΔIS903 or ISEcp1-IS10 -bla(CTX-M-14) -ΔIS903) and a downstream IS26 in 14 (14.4%) isolates. For the remaining two isolates, fosA3 was flanked by a downstream IS26 but the upstream part cannot be defined. In a random subset of 18 isolates, fosA3 was carried on transferable plasmids with sizes of 50-200 kb and the following replicons: F2:A-B- (n = 3), F16:A1:B- (n = 2), F24:A-B- (n = 1), N (n = 1), B/O (n = 1) and untypeable (n = 3). SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates the emergence of fosA3-mediated fosfomycin resistance among multidrug-resistant E. coli isolates from various animals. IS26 transposon-like structures might be the main vehicles for dissemination of fosA3. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/23216653/Dissemination_of_plasmid_mediated_fosfomycin_resistance_fosA3_among_multidrug_resistant_Escherichia_coli_from_livestock_and_other_animals_ L2 - https://doi.org/10.1111/jam.12099 DB - PRIME DP - Unbound Medicine ER -