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Perivagal antagonist treatment in rats selectively blocks the reflex and afferent responses of vagal lung C fibers to intravenous agonists.
J Appl Physiol (1985). 2013 Feb; 114(3):361-70.JA

Abstract

The terminals of vagal lung C fibers (VLCFs) express various types of pharmacological receptors that are important to the elicitation of airway reflexes and the development of airway hypersensitivity. We investigated the blockade of the reflex and afferent responses of VLCFs to intravenous injections of agonists using perivagal treatment with antagonists (PAT) targeting the transient receptor potential vanilloid 1, P2X, and 5-HT(3) receptors in anesthetized rats. Blockading these responses via perivagal capsaicin treatment (PCT), which blocks the neural conduction of C fibers, was also studied. We used capsaicin, α,β-methylene-ATP, and phenylbiguanide as the agonists, and capsazepine, iso-pyridoxalphosphate-6-azophenyl-2',5'-disulfonate, and tropisetron as the antagonists of transient receptor potential vanilloid 1, P2X, and 5-HT(3) receptors, respectively. We found that each of the PATs abolished the VLCF-mediated reflex apnea evoked by the corresponding agonist, while having no effect on the response to other agonists. Perivagal vehicle treatment failed to produce any such blockade. These blockades had partially recovered at 3 h after removal of the PATs. In contrast, PCT abolished the reflex apneic response to all three agonists. Both PATs and PCT did not affect the myelinated afferent-mediated apneic response to lung inflation. Consistently, our electrophysiological studies revealed that each of the PATs prevented the VLCF responses to the corresponding agonist, but not to any other agonist. PCT inevitably prevented the VLCF responses to all three agonists. Thus these PATs selectively blocked the stimulatory action of corresponding agonists on the VLCF terminals via mechanisms that are distinct from those of PCT. PAT may become a novel intervention for studying the pharmacological modulation of VLCFs.

Authors+Show Affiliations

Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23221955

Citation

Lin, Yu-Jung, et al. "Perivagal Antagonist Treatment in Rats Selectively Blocks the Reflex and Afferent Responses of Vagal Lung C Fibers to Intravenous Agonists." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 114, no. 3, 2013, pp. 361-70.
Lin YJ, Lin YS, Lai CJ, et al. Perivagal antagonist treatment in rats selectively blocks the reflex and afferent responses of vagal lung C fibers to intravenous agonists. J Appl Physiol (1985). 2013;114(3):361-70.
Lin, Y. J., Lin, Y. S., Lai, C. J., Yuan, Z. F., Ruan, T., & Kou, Y. R. (2013). Perivagal antagonist treatment in rats selectively blocks the reflex and afferent responses of vagal lung C fibers to intravenous agonists. Journal of Applied Physiology (Bethesda, Md. : 1985), 114(3), 361-70. https://doi.org/10.1152/japplphysiol.00977.2012
Lin YJ, et al. Perivagal Antagonist Treatment in Rats Selectively Blocks the Reflex and Afferent Responses of Vagal Lung C Fibers to Intravenous Agonists. J Appl Physiol (1985). 2013;114(3):361-70. PubMed PMID: 23221955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Perivagal antagonist treatment in rats selectively blocks the reflex and afferent responses of vagal lung C fibers to intravenous agonists. AU - Lin,Yu-Jung, AU - Lin,You Shuei, AU - Lai,Ching Jung, AU - Yuan,Zung Fan, AU - Ruan,Ting, AU - Kou,Yu Ru, Y1 - 2012/12/06/ PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2013/11/16/medline SP - 361 EP - 70 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 114 IS - 3 N2 - The terminals of vagal lung C fibers (VLCFs) express various types of pharmacological receptors that are important to the elicitation of airway reflexes and the development of airway hypersensitivity. We investigated the blockade of the reflex and afferent responses of VLCFs to intravenous injections of agonists using perivagal treatment with antagonists (PAT) targeting the transient receptor potential vanilloid 1, P2X, and 5-HT(3) receptors in anesthetized rats. Blockading these responses via perivagal capsaicin treatment (PCT), which blocks the neural conduction of C fibers, was also studied. We used capsaicin, α,β-methylene-ATP, and phenylbiguanide as the agonists, and capsazepine, iso-pyridoxalphosphate-6-azophenyl-2',5'-disulfonate, and tropisetron as the antagonists of transient receptor potential vanilloid 1, P2X, and 5-HT(3) receptors, respectively. We found that each of the PATs abolished the VLCF-mediated reflex apnea evoked by the corresponding agonist, while having no effect on the response to other agonists. Perivagal vehicle treatment failed to produce any such blockade. These blockades had partially recovered at 3 h after removal of the PATs. In contrast, PCT abolished the reflex apneic response to all three agonists. Both PATs and PCT did not affect the myelinated afferent-mediated apneic response to lung inflation. Consistently, our electrophysiological studies revealed that each of the PATs prevented the VLCF responses to the corresponding agonist, but not to any other agonist. PCT inevitably prevented the VLCF responses to all three agonists. Thus these PATs selectively blocked the stimulatory action of corresponding agonists on the VLCF terminals via mechanisms that are distinct from those of PCT. PAT may become a novel intervention for studying the pharmacological modulation of VLCFs. SN - 1522-1601 UR - https://www.unboundmedicine.com/medline/citation/23221955/Perivagal_antagonist_treatment_in_rats_selectively_blocks_the_reflex_and_afferent_responses_of_vagal_lung_C_fibers_to_intravenous_agonists_ L2 - https://journals.physiology.org/doi/10.1152/japplphysiol.00977.2012?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -