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Activation of epidermal toll-like receptor 2 enhances tight junction function: implications for atopic dermatitis and skin barrier repair.
J Invest Dermatol 2013; 133(4):988-98JI

Abstract

Atopic dermatitis (AD) is characterized by epidermal tight junction (TJ) defects and a propensity for Staphylococcus aureus skin infections. S. aureus is sensed by many pattern recognition receptors, including Toll-like receptor 2 (TLR2). We hypothesized that an effective innate immune response will include skin barrier repair, and that this response is impaired in AD subjects. S. aureus-derived peptidoglycan (PGN) and synthetic TLR2 agonists enhanced TJ barrier and increased expression of TJ proteins, claudin-1 (CLDN1), claudin-23 (CLDN23), occludin, and Zonulae occludens 1 (ZO-1) in primary human keratinocytes. A TLR2 agonist enhanced skin barrier recovery in human epidermis wounded by tape stripping. Tlr2(-/-) mice had a delayed and incomplete barrier recovery following tape stripping. AD subjects had reduced epidermal TLR2 expression as compared with nonatopic subjects, which inversely correlated (r=-0.654, P=0.0004) with transepidermal water loss (TEWL). These observations indicate that TLR2 activation enhances skin barrier in murine and human skin and is an important part of a wound repair response. Reduced epidermal TLR2 expression observed in AD patients may have a role in their incompetent skin barrier.

Authors+Show Affiliations

Department of Dermatology, University of Rochester Medical Center, Rochester, NY, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

23223142

Citation

Kuo, I-Hsin, et al. "Activation of Epidermal Toll-like Receptor 2 Enhances Tight Junction Function: Implications for Atopic Dermatitis and Skin Barrier Repair." The Journal of Investigative Dermatology, vol. 133, no. 4, 2013, pp. 988-98.
Kuo IH, Carpenter-Mendini A, Yoshida T, et al. Activation of epidermal toll-like receptor 2 enhances tight junction function: implications for atopic dermatitis and skin barrier repair. J Invest Dermatol. 2013;133(4):988-98.
Kuo, I. H., Carpenter-Mendini, A., Yoshida, T., McGirt, L. Y., Ivanov, A. I., Barnes, K. C., ... Beck, L. A. (2013). Activation of epidermal toll-like receptor 2 enhances tight junction function: implications for atopic dermatitis and skin barrier repair. The Journal of Investigative Dermatology, 133(4), pp. 988-98. doi:10.1038/jid.2012.437.
Kuo IH, et al. Activation of Epidermal Toll-like Receptor 2 Enhances Tight Junction Function: Implications for Atopic Dermatitis and Skin Barrier Repair. J Invest Dermatol. 2013;133(4):988-98. PubMed PMID: 23223142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of epidermal toll-like receptor 2 enhances tight junction function: implications for atopic dermatitis and skin barrier repair. AU - Kuo,I-Hsin, AU - Carpenter-Mendini,Amanda, AU - Yoshida,Takeshi, AU - McGirt,Laura Y, AU - Ivanov,Andrei I, AU - Barnes,Kathleen C, AU - Gallo,Richard L, AU - Borkowski,Andrew W, AU - Yamasaki,Kenshi, AU - Leung,Donald Y, AU - Georas,Steve N, AU - De Benedetto,Anna, AU - Beck,Lisa A, Y1 - 2012/12/06/ PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2013/5/8/medline SP - 988 EP - 98 JF - The Journal of investigative dermatology JO - J. Invest. Dermatol. VL - 133 IS - 4 N2 - Atopic dermatitis (AD) is characterized by epidermal tight junction (TJ) defects and a propensity for Staphylococcus aureus skin infections. S. aureus is sensed by many pattern recognition receptors, including Toll-like receptor 2 (TLR2). We hypothesized that an effective innate immune response will include skin barrier repair, and that this response is impaired in AD subjects. S. aureus-derived peptidoglycan (PGN) and synthetic TLR2 agonists enhanced TJ barrier and increased expression of TJ proteins, claudin-1 (CLDN1), claudin-23 (CLDN23), occludin, and Zonulae occludens 1 (ZO-1) in primary human keratinocytes. A TLR2 agonist enhanced skin barrier recovery in human epidermis wounded by tape stripping. Tlr2(-/-) mice had a delayed and incomplete barrier recovery following tape stripping. AD subjects had reduced epidermal TLR2 expression as compared with nonatopic subjects, which inversely correlated (r=-0.654, P=0.0004) with transepidermal water loss (TEWL). These observations indicate that TLR2 activation enhances skin barrier in murine and human skin and is an important part of a wound repair response. Reduced epidermal TLR2 expression observed in AD patients may have a role in their incompetent skin barrier. SN - 1523-1747 UR - https://www.unboundmedicine.com/medline/citation/23223142/Activation_of_epidermal_toll_like_receptor_2_enhances_tight_junction_function:_implications_for_atopic_dermatitis_and_skin_barrier_repair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)36206-0 DB - PRIME DP - Unbound Medicine ER -