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Transmission ratio distortion of molecular markers in a doubled haploid population originated from a natural hybrid between Osmunda japonica and O. lancea.
J Plant Res. 2013 Jul; 126(4):469-82.JP

Abstract

In ferns, intra-gametophytic selfing occurs as a mode of reproduction where two gametes from the same gametophyte form a completely homozygous sporophyte. Intra-gametophytic selfing is considered to be prevented by lethal or deleterious recessive genes in several diploid species. In order to investigate the modes and tempo of selection acting different developmental stages, doubled haploids obtained from intra-gametophytic selfing within isolated gametophytes of a putative F1 hybrid between Osmunda japonica and O. lancea were analyzed with EST_derived molecular markers, and the distribution pattern of transmission ratio distortion (TRD) along linkage map was clarified. As the results, the markers with skewness were clustered in two linkage groups. For the two highly distorted regions, gametophytes and F2 population were also examined. The markers skewed towards O. japonica on a linkage group (LG_2) showed skewness also in gametophytes, and the TRD was generated in the process of spore formation or growth of gametophytes. Also, selection appeared to be operating in the gametophytic stage. The markers on other linkage group (LG_11) showed highest skewness towards O. lancea in doubled haploids, and it was suggested that the segregation of LG_11 were influenced by zygotic lethality or genotypic evaluation and that some deleterious recessive genes exist in LG_11 and reduce the viability of homozygotes with O. japonica alleles. It is very likely that a region of LG_11were responsible for the low frequencies of intra-gametophytic selfing in O. japonica.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yatabe-Kakugawa Y
Botanical Gardens, Graduate School of Science, The University of Tokyo, 3-7-1 Hakusan, Bunkyo-ku, Tokyo, 112-0001, Japan. ykakugawa@ns.bg.s.u-tokyo.ac.jp
Tsutsumi C
No affiliation info available
Hirayama Y
No affiliation info available
Tsuneki S
No affiliation info available
Murakami N
No affiliation info available
Kato M
No affiliation info available

MeSH

AllelesChimeraCluster AnalysisCytoplasmDNA, ChloroplastDNA, PlantFernsGene FrequencyGenetic LinkageGenetic LociGenetic MarkersGenotypeGerm Cells, PlantHaploidyInbreedingReproductionSelf-FertilizationSequence Analysis, DNA

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23224293

Citation

Yatabe-Kakugawa, Yoko, et al. "Transmission Ratio Distortion of Molecular Markers in a Doubled Haploid Population Originated From a Natural Hybrid Between Osmunda Japonica and O. Lancea." Journal of Plant Research, vol. 126, no. 4, 2013, pp. 469-82.
Yatabe-Kakugawa Y, Tsutsumi C, Hirayama Y, et al. Transmission ratio distortion of molecular markers in a doubled haploid population originated from a natural hybrid between Osmunda japonica and O. lancea. J Plant Res. 2013;126(4):469-82.
Yatabe-Kakugawa, Y., Tsutsumi, C., Hirayama, Y., Tsuneki, S., Murakami, N., & Kato, M. (2013). Transmission ratio distortion of molecular markers in a doubled haploid population originated from a natural hybrid between Osmunda japonica and O. lancea. Journal of Plant Research, 126(4), 469-82. https://doi.org/10.1007/s10265-012-0540-4
Yatabe-Kakugawa Y, et al. Transmission Ratio Distortion of Molecular Markers in a Doubled Haploid Population Originated From a Natural Hybrid Between Osmunda Japonica and O. Lancea. J Plant Res. 2013;126(4):469-82. PubMed PMID: 23224293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transmission ratio distortion of molecular markers in a doubled haploid population originated from a natural hybrid between Osmunda japonica and O. lancea. AU - Yatabe-Kakugawa,Yoko, AU - Tsutsumi,Chie, AU - Hirayama,Yumiko, AU - Tsuneki,Shizuka, AU - Murakami,Noriaki, AU - Kato,Masahiro, Y1 - 2012/12/06/ PY - 2012/07/30/received PY - 2012/11/11/accepted PY - 2012/12/11/entrez PY - 2012/12/12/pubmed PY - 2014/2/25/medline SP - 469 EP - 82 JF - Journal of plant research JO - J Plant Res VL - 126 IS - 4 N2 - In ferns, intra-gametophytic selfing occurs as a mode of reproduction where two gametes from the same gametophyte form a completely homozygous sporophyte. Intra-gametophytic selfing is considered to be prevented by lethal or deleterious recessive genes in several diploid species. In order to investigate the modes and tempo of selection acting different developmental stages, doubled haploids obtained from intra-gametophytic selfing within isolated gametophytes of a putative F1 hybrid between Osmunda japonica and O. lancea were analyzed with EST_derived molecular markers, and the distribution pattern of transmission ratio distortion (TRD) along linkage map was clarified. As the results, the markers with skewness were clustered in two linkage groups. For the two highly distorted regions, gametophytes and F2 population were also examined. The markers skewed towards O. japonica on a linkage group (LG_2) showed skewness also in gametophytes, and the TRD was generated in the process of spore formation or growth of gametophytes. Also, selection appeared to be operating in the gametophytic stage. The markers on other linkage group (LG_11) showed highest skewness towards O. lancea in doubled haploids, and it was suggested that the segregation of LG_11 were influenced by zygotic lethality or genotypic evaluation and that some deleterious recessive genes exist in LG_11 and reduce the viability of homozygotes with O. japonica alleles. It is very likely that a region of LG_11were responsible for the low frequencies of intra-gametophytic selfing in O. japonica. SN - 1618-0860 UR - https://www.unboundmedicine.com/medline/citation/23224293/Transmission_ratio_distortion_of_molecular_markers_in_a_doubled_haploid_population_originated_from_a_natural_hybrid_between_Osmunda_japonica_and_O__lancea_ L2 - https://dx.doi.org/10.1007/s10265-012-0540-4 DB - PRIME DP - Unbound Medicine ER -